Novel spectroscopy method to reveal optimal culture conditions in Escherichia coli fermenter DOI
Jia Chen,

Huakun Wu,

Jingru Chen

et al.

Letters in Applied Microbiology, Journal Year: 2024, Volume and Issue: 77(12)

Published: Nov. 30, 2024

Abstract Fermentation engineering is critical for mass-producing chemicals, food additives, and medicines, where optimal culture conditions maximize microbial growth metabolite production. Although monitoring bacterial during fermentation critical, there a lack of non-invasive sensitive method to directly monitor the metabolism. In this paper, novel optical proposed based on tunable diode laser absorption spectroscopy. First, detecting system consisting laser, detection, homemade board, an incubator established verified be able production CO2 in Escherichia coli through 25-h detection period. Second, quantitative rate analysis specified by calculating threshold time (TT) intervals between consecutive dilution gradients, with least sum residuals chosen as threshold. Finally, alongside varied pH temperature settings simulated fermenter, we elucidated influence these factors E. metabolism curves calculated rates via TT, identifying 38°C 7.0 pH. This study presents approach reveal holding promises online real-time future.

Language: Английский

Microbial Community Structure in the Taklimakan Desert: The Importance of Nutrient Levels in Medium and Culture Methods DOI Creative Commons

Feng Wen,

Siyuan Wu, Xiaoxia Luo

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(10), P. 797 - 797

Published: Oct. 6, 2024

Although the Taklimakan Desert lacks necessary nutrients and conditions to support an extensive ecosystem, it is a treasure trove of extremophile resources with special structures functions. We analyzed bacterial communities using oligotrophic medium velvet cloth replicate combined extended culture duration. isolated numerous uncultured microorganisms rare belonging genera not often or recently described, such as

Language: Английский

Citations

2
Novel spectroscopy method to reveal optimal culture conditions in Escherichia coli fermenter DOI

Huakun Wu,

Jingru Chen,

Jia Zheng

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 3, 2024

Abstract Fermentation engineering has played a pivotal role in modern industry for mass-producing chemicals, food additives, and medicines, with optimal culture conditions crucial maximizing microbial growth metabolite production. Thus, bacteria monitoring was fermentation processes, current methods falling into two categories: off-line sampling in-situ on-line monitoring. While suffered from discrete points potential pollution, faced limitations including an inability to distinguish living dead cells impurities lack of direct representation metabolism. Carbon dioxide (CO2) levels, which closely correlated chemical synthesis, could be measured high sensitivity Tunable Diode Laser Absorption Spectroscopy (TDLAS) technology, enabling non-invasive, high-sensitivity detection CO2 concentrations fermentation, offering powerful tool optimizing enhancing production efficiency. This study presented novel approach reveal the Escherichia coli (E. coli) fermentation. Through implementation custom metabolism system based on TDLAS technology simulated fermenter, alongside varied pH temperature settings, we elucidated influence these factors E. curves calculated rates via threshold times, identifying 38°C as 7.5 pH. Integration this spectroscopy method fermenters held promise enhanced situ online real-time future fermentations.

Language: Английский

Citations

0
Comparison of Incubation Conditions for Microbial Contaminant Isolation in Microbiological Environmental Monitoring DOI Creative Commons
О. В. Гунар, Н. Г. Сахно,

O. S. Tyncherova

et al.

Regulâtornye issledovaniâ i èkspertiza lekarstvennyh sredstv., Journal Year: 2024, Volume and Issue: 14(4), P. 483 - 492

Published: Aug. 28, 2024

INTRODUCTION . Microbiological environmental monitoring programmes for clean rooms pharmaceutical production vary depending on the regulatory document. This is particularly evident in experimental conditions, including culture media used sampling, as well temperature and time of incubation. To harmonise quality control procedures, it necessary to develop a unified strategy processing microbiological samples. AIM. study aimed investigate optimal conditions sample incubation during rooms.MATERIALS AND METHODS. The compared several indicator microorganisms, Bacillus subtilis ATCC 6633, Staphylococcus aureus 6538, Pseudomonas aeruginosa 9027, Candida albicans 10231, Aspergillus brasiliensis 16404, fumigatus F-62, terreus F-1269, Penicillium chrysogenum F-3 (the latter three strains were obtained from Russian National Collection Industrial Microorganisms), isolates, epidermidis, Kocuria rosea, Micrococcus luteus, spp., Sphingomonas paucimobilis. trypticase soy agar (TSA), Sabouraud's dextrose chloramphenicol (SDCA), Reasoner’s 2A (R2A). regimes follows: 2 days at 30–35 ºC then 3 20–25 ºC; 48–72 hours (for aerobic bacteria); 5–7 yeasts moulds). RESULTS comparison showed no statistically significant differences between results with TSA R2A under different temperatureconditions. germination rates isolates grown significantly lower (by 19–37%) two-tiered scheme that started temperature. identified groups microorganisms requiring special (bacteria suppressed physiological functions CONCLUSIONS. confirmed need standardise testing provide its proper regulation by drafting general monograph matter. authors demonstrated applicability both monitoring. Currently, use one non-selective medium requires validation case-by-case basis. Although sequence levels did not affect significantly, regime starting higher (30–35 ºС) was determined preferable bacterial isolates.

Language: Английский

Citations

0
Co-doped ZnO nanofibers fabricated via electrospinning for rapid and ppb-level detection of listeria biomarker 3-hydroxy-2-butanone DOI
Yan Li,

G. Y. Song,

Xiaoxue Lian

et al.

Ceramics International, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Language: Английский

Citations

0
Novel spectroscopy method to reveal optimal culture conditions in Escherichia coli fermenter DOI
Jia Chen,

Huakun Wu,

Jingru Chen

et al.

Letters in Applied Microbiology, Journal Year: 2024, Volume and Issue: 77(12)

Published: Nov. 30, 2024

Abstract Fermentation engineering is critical for mass-producing chemicals, food additives, and medicines, where optimal culture conditions maximize microbial growth metabolite production. Although monitoring bacterial during fermentation critical, there a lack of non-invasive sensitive method to directly monitor the metabolism. In this paper, novel optical proposed based on tunable diode laser absorption spectroscopy. First, detecting system consisting laser, detection, homemade board, an incubator established verified be able production CO2 in Escherichia coli through 25-h detection period. Second, quantitative rate analysis specified by calculating threshold time (TT) intervals between consecutive dilution gradients, with least sum residuals chosen as threshold. Finally, alongside varied pH temperature settings simulated fermenter, we elucidated influence these factors E. metabolism curves calculated rates via TT, identifying 38°C 7.0 pH. This study presents approach reveal holding promises online real-time future.

Language: Английский

Citations

0