Journal of genetics and genomics/Journal of Genetics and Genomics, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 1, 2024
Language: Английский
Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16
Published: Feb. 20, 2025
The RNA-based spray-induced gene silencing (SIGS) technology represents an ecologically sustainable approach to crop protection and pathogen management. Following the recent approval of Ledprona as first sprayable double-stranded RNA (dsRNA) biopesticide by EPA at end 2023, SIGS has emerged a focal point in both academic industrial sectors. This review analyzes advances emerging trends SIGS. application for protection, including control insects, fungal pathogens, viruses, is briefly summarized. Distinguishing this from others, we delve into practical aspects technology, such selection screening target genes, large-scale production methods, delivery systems, highlighting major advancements these areas also addressing remaining questions issues, particularly concerning safety concerns controlling harmful weeds. Finally, emphasizes its integration with nanotechnology other methodologies. Collectively, rapid progress studies poised accelerate maturation technology.
Language: Английский
Citations
0
Agriculture, Journal Year: 2025, Volume and Issue: 15(6), P. 642 - 642
Published: March 18, 2025
Concerns about the environmental and health risks of synthetic insecticides are driving search for alternative pest control methods. Leaf-cutting ants (LCAs), one most significant pests in neotropics, cause substantial economic damage to agriculture present challenges due their complex biology ecology. While chemical remains primary strategy, its intensive use has negative impacts, promotes resistance, endangers non-target species, including plants, animals, humans. This review describes LCAs, examines traditional methods suggests strategies such as entomopathogenic fungi (EPFs) combined with sublethal doses insecticides, plant essential oils (EOs), RNAi techniques. Here, we emphasize need address LCA management sustainably by investigating ecology at both “colony” “individual” levels. Colony-level factors include morphology, life cycle, behavior, division labor, nest structure, while individual-level mechanisms involve sensory, biochemical, behavioral adaptations garden sterilization decontamination. also highlights potential insecticide EPFs induce changes worker mortality, it details mode action EOs promising strategies. The integration biological approaches could offer sustainable alternatives insecticides.
Language: Английский
Citations
0
Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 210, P. 106392 - 106392
Published: March 24, 2025
Language: Английский
Citations
0
Microbiological Research, Journal Year: 2025, Volume and Issue: unknown, P. 128178 - 128178
Published: April 1, 2025
Language: Английский
Citations
0
aBIOTECH, Journal Year: 2025, Volume and Issue: unknown
Published: April 14, 2025
Language: Английский
Citations
0
Frontiers in Environmental Science, Journal Year: 2024, Volume and Issue: 12
Published: Oct. 23, 2024
Each new class of insecticides that emerged during the development plant protection gradually found most suitable group insect pests for application. At same time, each individual insecticide, a balance was sought between its effectiveness, on one hand, and safety non-target organisms ecosystem as whole, other hand. Neonicotinoids, diamides pyrethroids, effective control agents, dominate insecticide market, but do not have outstanding performance in selectivity biodegradation. The biodegradation is important indicators, representing what will be said about hidden costs resulting harvest paid by environment human health. Oligonucleotide (contact unmodified antisense DNA (CUAD) biotechnology, or ‘genetic zipper’ method) RNA biocontrols (double-stranded technology) natural polymers next-generation classes possess unique characteristics fast high action. While current chemical require days, months even years bacteria fungi, oligonucleotide are substantially biodegraded within hours presence nucleases. Nucleic acid-based potential to complement existing market set an eco-precedent crop products where effectiveness determined organisms, factors being equal, choice particular agent biodegradability. It should noted single once appeared has completely disappeared; rather, it occupied niche, declining under pressure insecticides. common trend towards use with higher biodegradability, which gives hope safer future planet.
Language: Английский
Citations
1
Viruses, Journal Year: 2024, Volume and Issue: 17(1), P. 49 - 49
Published: Dec. 31, 2024
The increasing challenges posed by plant viral diseases demand innovative and sustainable management strategies to minimize agricultural losses. Exogenous double-stranded RNA (dsRNA)-mediated interference (RNAi) represents a transformative approach combat pathogens without the need for genetic transformation. This review explores mechanisms underlying dsRNA-induced RNAi, highlighting its ability silence specific genes through small interfering RNAs (siRNAs). Key advancements in dsRNA production, including cost-effective microbial synthesis vitro methods, are examined alongside delivery techniques such as spray-induced gene silencing (SIGS) nanocarrier-based systems. Strategies enhancing stability, use of nanomaterials like layered double hydroxide nanosheets carbon dots, discussed address environmental degradation challenges. Practical applications this technology against various viruses potential ensure food security emphasized. also delves into regulatory considerations, risk assessments, associated with off-target effects pathogen resistance. By evaluating both opportunities limitations, underscores role exogenous solution achieving disease resistance plants.
Language: Английский
Citations
1
Advanced Biotechnology, Journal Year: 2024, Volume and Issue: 2(4)
Published: Nov. 1, 2024
Language: Английский
Citations
0
Advanced Science, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 18, 2024
Unlike animals, plants are unable to move and lack specialized immune cells circulating antibodies. As a result, they always threatened by large number of microbial pathogens harmful pests that can significantly reduce crop yield worldwide. Therefore, the development new strategies control them is essential mitigate increasing risk crops lost plant diseases. Recent developments in genetic engineering, including efficient gene manipulation transformation methods, editing synthetic biology, coupled with understanding pathogenicity immunity, both at molecular genomic levels, have enhanced capabilities develop disease resistance plants. This review comprehensively explains fundamental mechanisms underlying tug-of-war between hosts, provides detailed overview different for developing Additionally, it summary potential genes be employed breeding key combat wide range pests, fungi, oomycetes, bacteria, viruses, nematodes, insects. Furthermore, this addresses limitations associated these their possible solutions. Finally, discusses future perspectives producing durable broad-spectrum resistance.
Language: Английский
Citations
0
Journal of genetics and genomics/Journal of Genetics and Genomics, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 1, 2024
Language: Английский
Citations
0