Efficient biological funneling of lignin into 2-pyrone-4,6-dicarboxylic acid via electrocatalytic depolymerization and genetically engineered Pseudomonas putida KT2440

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 141657 - 141657

Published: March 1, 2025

Language: Английский

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Integrated Metabolomic and Transcriptomic Analysis Reveals the Pharmacological Effects and Differential Mechanisms of Isoflavone Biosynthesis in Four Species of Glycyrrhiza

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2539 - 2539

Published: March 12, 2025

Licorice (Glycyrrhiza L.) is a globally popular medicinal and edible plant, with nearly 30 species distributed across all continents. The usable part primarily the root. To understand metabolic differences among different Glycyrrhiza species, we selected four performed comprehensive analyses of their roots. Metabolomic profiling was conducted using UPLC-MS/MS GC-MS, while transcriptomic analysis carried out RNA-sequencing. A total 2716 metabolites were identified, including flavonoids (527 types) terpenoids (251 types), various other components. Subsequently, network pharmacology employed to explore value potential pharmacological ingredients these metabolites. Joint metabolomic data revealed significant in differentially accumulated (DAMs) expressed genes (DEGs) pairwise comparisons species. These enriched isoflavone pathway. Further investigation into regulatory mechanisms biosynthesis identified key involved biosynthesis. Finally, made reasonable predictions suitable habitats for aiming provide new insights development utilization licorice resources. results this study can serve as basis in-depth research on regulation licorice.

Language: Английский

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Habitat island biogeography of mountaintop plant and soil microbiomes: similar patterns driven by different mechanisms

Global Ecology and Conservation, Journal Year: 2025, Volume and Issue: unknown, P. e03574 - e03574

Published: April 1, 2025

Language: Английский

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The Integration of Machine Learning into Proteomics Advances Food Authentication and Adulteration Control

Trends in Food Science & Technology, Journal Year: 2025, Volume and Issue: unknown, P. 105029 - 105029

Published: April 1, 2025

Language: Английский

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Virgifigura deserti gen. nov., sp. nov., isolated from the Gurbantunggut Desert soil

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Journal Year: 2025, Volume and Issue: 75(4)

Published: April 28, 2025

A Gram-stain-negative, aerobic, rod-shaped bacterial strain, SYSU D60014 T , was isolated from a sandy soil sample collected the Gurbantunggut Desert in Xinjiang, PR China. Colonies of were pink-coloured, crystalline, irregular edge with rough surface. Phylogenetic analyses based on both 16S rRNA gene and whole-genome sequences assigned strain belonged to family Rhodospirillaceae formed distinct lineage showed 92.5–91.0% similarity closely related strains. Digital DNA–DNA hybridization, average nucleotide identity amino acid values between members its species 17.2–19.6%, 70.8–74.4% 48.9–64.5%, respectively. The complete genome 5,100,926 bp DNA G+C content 65.1%. Cells oxidase-positive catalase-negative. could grow at 28–40 °C (optimum, 37 °C), pH 5.0–8.0 7.0), presence up 3% NaCl 1–1.5%, w/v) R2A. predominant menaquinone Q-10. main polar lipids phosphatidylethanolamine, phosphatidylmethyl ethanolamine, diphosphatidylglycerol, unidentified aminophospholipid, aminolipid, phospholipid, four two phosphoglycolipids. Major cellular fatty acids C 19 : 0 cyclo ω 8 c summed feature (C 18 1 ω7 and/or ω6 c) 16 . Based results obtained through genotypic phenotypic analyses, we propose that represents novel genus within for which name Virgifigura deserti gen. nov., sp. nov. (type =NBRC 112951 =CPCC 101030 ).

Language: Английский

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Trichoderma afroharzianum T22 Induces Rhizobia and Flavonoid‐Driven Symbiosis to Promote Tolerance to Alkaline Stress in Garden Pea

Plant Cell & Environment, Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

ABSTRACT Soil alkalinity is a limiting factor for crops, yet the role of beneficial fungi in mitigating this abiotic stress garden pea understudied. In study, Trichoderma afroharzianum T22 colonised roots cultivars exposed to soil host‐specific manner. alkaline‐exposed Sugar Snap, improved growth parameters, consistent with increased tissue mineral content, particularly Fe and Mn, as well enhanced rhizosphere siderophore levels. The split‐root assay demonstrated that effects on alkaline mitigation are result whole‐plant association rather than localised root‐specific effects. RNA‐seq analysis showed 575 818 differentially expressed genes upregulated downregulated inoculated under conditions. were mostly involved flavonoid biosynthetic pathway (monooxygenase activity, ammonia‐lyase 4‐coumarate‐CoA ligase), along related transport redox homoeostasis. Further, precursor restored plant health even absence T22, confirming microbial symbiosis stress. Interestingly, abundance rhizobia, Rhizobium leguminosarum indicum , induction NifA NifD NifH nodules, suggesting connection between rhizobia alkalinity. elevated siderophore, root flavonoid, expression PsCoA (4‐coumarate‐CoA ligase) relative TaAOX1 R. diminished when was substituted exogenous Fe. This suggests eliminates need microbiome‐driven mobilisation, while T22‐mediated depends flavonoid‐driven abundance. It further supported by positive interaction media. Thus, effect likely stems from their interactions, not solely status, Fe, plants. study provides first mechanistic insights into interactions host advancing microbiome strategies alleviate peas other legumes.

Language: Английский

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Trichoderma afroharzianuminduces rhizobia and flavonoid through systemic signaling to mitigate iron deficiency in garden pea

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 16, 2024

ABSTRACT Iron (Fe) deficiency is a limiting factor for legumes, yet the role of beneficial fungi in mitigating Fe garden pea understudied. In this study, Trichoderma afroharzianum T22 colonized roots cultivars exposed to alkali-mediated Fe-deficiency host- specific manner. Fe-deficient Sugar Snap, improved growth parameters consistent with increased tissue and rhizosphere siderophore. The split-root assay demonstrated that effects on mitigation are result whole-plant association rather than localized root-specific effects. RNA-seq analysis showed 575 818 differentially expressed genes upregulated downregulated inoculated under deficiency. were mostly involved flavonoid biosynthetic pathway (monooxygenase activity, ammonia-lyase 4-coumarate-CoA ligase), along related mineral transport redox homeostasis. Further, precursor restored plant health even absence T22, confirming microbial symbiosis Interestingly, abundance rhizobia, particularly Rhizobium leguminosarum indicum , induction NifA NifD NifH nodules, suggesting connection between rhizobia Fe-starvation. elevated siderophore, root flavonoid, expression PsCoA (4-coumarate-CoA ligase) as well relative TaAOX1 R. diminished when was substituted exogenous Fe. This suggests eliminates need microbiome-driven mobilization, while T22-mediated depends flavonoid- driven abundance. It further supported by positive interaction media. Thus, effect likely stems from their interactions, not just status plants. study provides first mechanistic insights into interactions host advancing microbiome strategies alleviate peas other legumes.

Language: Английский

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A century of innovation in life sciences at Sun Yat-sen University

Advanced Biotechnology, Journal Year: 2024, Volume and Issue: 2(4)

Published: Nov. 1, 2024

Language: Английский

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Nocardioides xinjiangensis sp. nov., a novel species isolated from desert soil

Antonie van Leeuwenhoek, Journal Year: 2024, Volume and Issue: 118(1)

Published: Nov. 25, 2024

Language: Английский

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