Addressing the Global Threat of Multidrug-Resistant Infections: The Role of Ceftazidime-Avibactam Revisited

Cureus, Journal Year: 2024, Volume and Issue: unknown

Published: May 13, 2024

Background and objective Bloodstream infections (BSIs) due to multidrug-resistant Gram-negative bacteria (MDR-GNB) pose a significant global health threat amid rising antimicrobial resistance (AMR). This study aimed investigate the efficacy of ceftazidime-avibactam (CZA) as therapeutic option for these infections, addressing urgent need novel treatments. Materials methods was conducted over one year in Department Microbiology, JSS Medical College Hospital, Mysuru, India, employed laboratory-based prospective design. From total 376 positive blood cultures, 147 (MDR) organisms were identified, 100 randomly selected final analysis. Susceptibility testing via disk diffusion minimum inhibitory concentration (MIC) determination performed evaluate CZA efficacy. Results

Language: Английский

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Saccharomyces yeast postbiotics mitigate mucosal damages from F18+ Escherichia coli challenges by positively balancing the mucosal microbiota in the jejunum of young pigs

Animal Microbiome, Journal Year: 2024, Volume and Issue: 6(1)

Published: Dec. 20, 2024

Abstract Background Enterotoxigenic Escherichia coli ( E. ) is one of the most prevalent causes diarrhea in young animals. Postbiotics derived from yeast have potential to positively influence mucosal microbiota jejunum, therefore it was hypothesized that Saccharomyces postbiotics could enhance and immune response mitigating effects infection with enterotoxigenic . The purpose this study investigate a postbiotic on jejunum newly weaned pigs challenged F18 + coli. Results Thirty-six individually housed nursery were allotted into three treatments utilizing randomized complete block design; negative control (NC: basal diet, no challenge), positive (PC: SYP (basal diet at 175 g/ton , challenge). On d 7, PC orally inoculated whereas NC received saline. 28, euthanized for sampling analyze microbiota, oxidative stress, status, intestinal morphology. reduced P < 0.05) growth performance compared NC. improved fecal score 7–18 when PC. protein carbonyl, gene expression Toll-like receptor 4, increased mammalian target rapamycin, Conclusions Challenge negatively impacted jejunal mucosa-associated morphology, affecting performance. reduce associated infection.

Language: Английский

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Metagenomic next-generation sequencing for the identification of infections caused by Gram-negative pathogens and the prediction of antimicrobial resistance

Laboratory Medicine, Journal Year: 2023, Volume and Issue: unknown

Published: May 30, 2023

Abstract Objective The aim of this study was to evaluate the efficacy metagenomic next-generation sequencing (mNGS) for identification Gram-negative bacteria (GNB) infections and prediction antimicrobial resistance. Methods A retrospective analysis conducted on 182 patients with diagnosis GNB who underwent mNGS conventional microbiological tests (CMTs). Results detection rate 96.15%, higher than CMTs (45.05%) a significant difference (χ 2 = 114.46, P &lt; .01). pathogen spectrum identified by significantly wider CMTs. Interestingly, substantially that (70.33% vs 23.08%, .01) in but not without antibiotic exposure. There positive correlation between mapped reads pro-inflammatory cytokines (interleukin-6 interleukin-8). However, failed predict resistance 5 12 compared phenotype susceptibility testing results. Conclusions Metagenomic has rate, spectrum, is less affected prior exposure identifying pathogens. may reflect state GNB-infected patients. Inferring actual phenotypes from data remains great challenge.

Language: Английский

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Curing of Citrobacter spp. Strains Antibiotic Resistance Harboring Plasmids Using Novel Physical, Chemical and Natural Substances

Egyptian Journal of Veterinary Science, Journal Year: 2024, Volume and Issue: 55(7), P. 2107 - 2118

Published: April 9, 2024

The current study was conducted on four isolates of Citrobacter freundii identified previously molecularly and registered in the Global GenBank under names ALGH1(accession No. OQ703592), ALGH2(accession OQ703593, ALGH3 (accession OQ703594), ALGH4(accession OQ703595), Antibiotics profile analysis achieved against 12 antibiotics, all showed 100% resistance to (tetracycline, Doxycycline oxytetracycline), whereas were sensitive Amikacin, Trimethoprim, Nalidixic acid Norfloxaine, Levofloxacine Ciprofloxacin. Curing experiments these plasmids by physical( high temperature), chemical (urea) natural substances using both onion garlic powder three concentration (1%,5%,10%), urea effective curing at concentrations 200 400 µg/ml. 45°C successed plasmids. To our knowledge this is considered first one which recorded plasmid curing. As a result, 5% 10% successful genes doxycycline Tetracycline, failed concentrations. For ALGH1,ALGH2&ALGH4 strains , while sensitivity appeared bacterial isolate spresented (Oxytetracycline) as agent (10%) resulted antibiotic (Tetracycline, Oxytetracycline, Doxycycline). This aimed cure from varying

Language: Английский

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Investigation of colistin resistance and method comparison in Klebsiella pneumoniae strains

Diagnostic Microbiology and Infectious Disease, Journal Year: 2024, Volume and Issue: 111(1), P. 116584 - 116584

Published: Oct. 30, 2024

Language: Английский

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Enterobacter cloacae as a Rare Cause of Cerebral Abscesses in a Neonatal Late-Onset Sepsis—a Case Report and Literature Review with Focus on Risk Factors, Diagnostics, and Treatment

Journal of Pediatric Infectious Diseases, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 19, 2024

Abstract Late-onset sepsis (LOS; occurring after the first 72 h of life) increases morbidity and mortality premature neonates, especially those with very low birth weight (≤1,500 g). In LOS, Enterobacter cloacae has been rarely described as a cause secondary brain abscesses. Our case report summarizes important aspects cerebral abscesses due to an E. in preterm neonate. patient received surgical abscess drainage rational antibiotic treatment, employing prolonged meropenem infusions plus fosfomycin subsequent treatment oral trimethoprim/sulfamethoxazole. Furthermore, we present review current literature from 2009 2022, comparing cases similar our case.

Language: Английский

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Pola Bakteri dan Sensitivitas Antibiotik pada Pasien Ulkus Kaki Diabetik di RSUP Dr. M. Djamil Padang

Jurnal Ilmu Kesehatan Indonesia, Journal Year: 2023, Volume and Issue: 4(3), P. 181 - 189

Published: Sept. 19, 2023

Latar Belakang: Ulkus kaki diabetik merupakan salah satu komplikasi diabetes melitus yang paling sering terjadi dan memiliki tingkat perawatan di rumah sakit tinggi. Salah penyebab ulkus adalah infeksi bakteri sehingga untuk penanganan diberikan antibiotik sesuai penyebabnya, tetapi awal dapat secara empiris. Objektif: Penelitian ini bertujuan mengetahui pola sensitivitasnya terhadap pada pasien dirawat Bangsal Penyakit Dalam RSUP Dr. M. Djamil Padang. Metode: Jenis penelitian deskriptif retrospektif dengan teknik pengambilan sampel total sampling. Sampel menggunakan data sekunder dari rekam medis Padang periode 2018–2021 35 sampel. dianalisis analisis univariat mendeskripsikan distribusi frekuensi. Hasil: Hasil didapatkan bahwa terbanyak perempuan usia ≤60 tahun. Bakteri berdasarkan kelompok Gram positif negatif negatif, yaitu Proteus sp (28,6%), Klebsiella (17,1%), Escherichia coli (8,6%), Acinetobacter baumannii (8,6%). aerob anareob aerob, Staphylococcus aureus Antibiotik sensitif meropenem, amikacin, trimethoprim/sulfamethoxazole. Kesimpulan: Kesimpulan menginfeksi negatif. sensitivitas tertinggi meropenem.

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