Development of extensive growth and growth boundary models for mesophilic and psychrotolerant Bacillus cereus in dairy products (Part 1)

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 21, 2025

Guidelines for combinations of product characteristics to prevent unacceptable growth Bacillus cereus in foods are lacking, and models therefore valuable predicting these responses. B. isolates dairy origin were used generate a comprehensive dataset develop two cardinal parameter boundary mesophilic psychrotolerant , respectively. Each model incorporated the inhibitory effect 11 environmental factors, i.e., temperature, pH, NaCl/a w organic acids (acetic, benzoic, citric, lactic, sorbic), phosphate salts (orthophosphate, diphosphate, triphosphate), interactions between factors. Cardinal values strain cocktails estimated using 231 203 maximum specific rates ( μ max values), respectively, generated standard liquid laboratory medium (BHI broth). Furthermore, an additional 113 100 dairy-specific (an ultra-filtration permeate from whey) evaluate responses obtained BHI broth. extensive selected conservatively data broth or UF permeate, such that widest range was each factor. The studied cocktail six vegetative exhibited greater acid tolerance than with lower pH min 4.75 versus 4.98), higher minimum concentrations MIC ) undissociated lactic u,LAC 2.99 2.34 mM) total citric T,CAC 169.1 82.5 mM). also had LAC remaining determined new can predict combined storage temperature wide characteristics, including melting salts. These support evaluation management subgroups various products. However, validation predictive is required determine their performance applicability.

Language: Английский

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Raw milk at refrigeration temperature displays an independent microbiota dynamic regardless Listeria monocytogenes contamination

Food Research International, Journal Year: 2025, Volume and Issue: 202, P. 115637 - 115637

Published: Jan. 1, 2025

Dairy products made of raw milk are associated with hypervirulent L. monocytogenes clonal complexes (CCs) CC1, CC4, and CC6, cause half the reported listeriosis outbreaks in Europe. However, it is currently unknown whether overrepresentation clones dairy conditioned by an alteration native microbiota growth and/or composition. In this study, lag phase, maximal rate, final concentration mesophilic aerobic bacteria from were measured at refrigerated temperature (4 °C) presence absence contamination. The composition dynamics evaluated (CC1, CC6), hypovirulent (CC9 CC121) 4 °C using 16S rRNA high-throughput sequencing. Our results showed that microbial communities naturally present independent contamination hyper- or CCs refrigeration temperature. Pseudomonas was most abundant genus on days 11 21, while Carnobacterium second regardless contaminant CCs. Altogether these suggest CC6 not consequence a differential microbiota.

Language: Английский

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AI’s Intelligence for Improving Food Safety: Only as Strong as the Data that Feeds It

Current Food Science and Technology Reports, Journal Year: 2025, Volume and Issue: 3(1)

Published: May 19, 2025

Language: Английский

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