Science Bulletin, Journal Year: 2021, Volume and Issue: 67(2), P. 117 - 118
Published: Aug. 11, 2021
Language: Английский
Current Biology, Journal Year: 2024, Volume and Issue: 34(3), P. 505 - 518.e6
Published: Jan. 11, 2024
Germ cells are essential to sexual reproduction. Across the animal kingdom, extracellular signaling isoprenoids, such as retinoic acids (RAs) in vertebrates and juvenile hormones (JHs) invertebrates, facilitate multiple processes Here we investigated role of these potent molecules embryonic germ cell development, using JHs Drosophila melanogaster a model system. In contrast their established endocrine roles during larval adult germline found that JH acts locally development. Using an vivo biosensor, observed active first within near primordial (PGCs) they migrate developing gonad. Through vitro assays, determined both necessary sufficient for PGC migration. Analysis into mechanisms this newly uncovered paracrine function revealed migration was compromised when were decreased or increased, suggesting specific titers spatiotemporal dynamics required robust colonization Compromised can impair fertility cause tumors many species, including humans. mammals, retinoids have development We like Drosophila, RA impact mouse vitro. Together, our study reveals previously unanticipated isoprenoids local effectors pre-gonadal suggests broadly shared mechanism
Language: Английский
Citations
19
Molecular and Cellular Endocrinology, Journal Year: 2024, Volume and Issue: 587, P. 112211 - 112211
Published: March 15, 2024
Language: Английский
Citations
14
TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 173, P. 117600 - 117600
Published: Feb. 23, 2024
Language: Английский
Citations
13
Proceedings of the National Academy of Sciences, Journal Year: 2021, Volume and Issue: 118(45)
Published: Oct. 25, 2021
Methyl farnesoate (MF) plays hormonal regulatory roles in crustaceans. An epoxidated form of MF, known as juvenile hormone (JH), controls metamorphosis and stimulates reproduction insects. To address the evolutionary significance MF epoxidation, we generated mosquitoes completely lacking either two enzymes that catalyze last steps MF/JH biosynthesis respectively: JH acid methyltransferase (JHAMT) P450 epoxidase CYP15 (EPOX). jhamt-/- larvae both died at onset metamorphosis. Strikingly, epox-/- mutants, which synthesized but no JH, completed entire life cycle. While adults were fertile, reproductive performance sexes was dramatically reduced. Our results suggest although can substitute for absence mosquitoes, it is with a significant fitness cost. We propose fulfill most its epoxidation to key innovation providing insects advantage.
Language: Английский
Citations
43
BMC Biology, Journal Year: 2021, Volume and Issue: 19(1)
Published: Oct. 8, 2021
Abstract Background The zinc-finger transcription factor Krüppel-homolog 1 (Kr-h1) exerts a dual regulatory role during insect development by preventing precocious larval/nymphal metamorphosis and in stimulating aspects of adult reproduction such as vitellogenesis. However, how Kr-h1 functions both transcriptional repressor juvenile an activator remains elusive. Here, we use the Locusta migratoria to dissect molecular mechanism which at these distinct developmental stages. Results We report that kinase PKCα triggers phosphorylation amino acid residue Ser 154 , step essential for its functions. During stage, phosphorylated recruits corepressor, C-terminal binding protein (CtBP). complex CtBP represses Ecdysone induced 93F ( E93 ) consequently prevents juvenile-to-adult transition. In insects, coactivator, CREB-binding (CBP), promotes vitellogenesis inducing expression Ribosomal L36 . Furthermore, with concomitant inhibition is evolutionarily conserved across orders. Conclusion Our results suggest indispensable recruitment cofactors, anti-metamorphic vitellogenic actions insects. data shed new light on understanding regulation function JH-regulated reproduction.
Language: Английский
Citations
27
FEBS Journal, Journal Year: 2023, Volume and Issue: 290(11), P. 2881 - 2894
Published: Jan. 7, 2023
Juvenile hormone (JH) signalling provides vital regulatory functions during insect development via transcriptional regulation of genes critical for the progression metamorphosis and oogenesis. Despite importance JH signalling, underlying molecular mechanisms remain largely unknown. Our current understanding pathway depends on static end‐point information suffers from lack time‐resolved data. Here, we have addressed dynamic aspect by monitoring in real time interactions receptor proteins. Use two tags that reconstitute a functional luciferase when proximity enabled us to follow rapid assembly heterodimer basic helix–loop–helix/Per‐Arnt‐SIM (bHLH‐PAS) proteins, methoprene‐tolerant (Met) taiman (Tai), upon specific binding Met. On similar timescale (minutes), dissociation Met‐Met complexes occurred, again strictly dependent Met interaction with agonist ligands. To resolve questions regarding role chaperone Hsp90/83 JHR complex formation, used same technique demonstrate Met‐Hsp83 persisted absence but readily dissociated Preincubation Hsp90 inhibitor geldanamycin showed protected degradation was produce active dimer Tai. Thus, appear be governed principles those regulating aryl hydrocarbon receptor, closest vertebrate homologue arthropod receptor.
Language: Английский
Citations
11
Published: March 3, 2025
Juvenile hormone (JH) is important to maintain insect larval status; however, its cell membrane receptor has not been identified. Using the lepidopteran Helicoverpa armigera (cotton bollworm), a serious agricultural pest, as model, we determined that tyrosine kinases (RTKs) cadherin 96ca (CAD96CA) and fibroblast growth factor homologue (FGFR1) function JH receptors by their roles in JH-regulated gene expression, status maintaining, rapid intracellular calcium increase, phosphorylation of MET1 cofactor Taiman, high affinity III. Gene knockout Cad96ca Fgfr1 CRISPR/Cas9 embryo knockdown various cells, overexpression CAD96CA FGFR1 mammalian HEK-293T cells all supported transmitting signal receptors.
Language: Английский
Citations
0
eLife, Journal Year: 2025, Volume and Issue: 13
Published: March 14, 2025
Juvenile hormone (JH) is important to maintain insect larval status; however, its cell membrane receptor has not been identified. Using the lepidopteran Helicoverpa armigera (cotton bollworm), a serious agricultural pest, as model, we determined that tyrosine kinases (RTKs) cadherin 96ca (CAD96CA) and fibroblast growth factor homologue (FGFR1) function JH receptors by their roles in JH-regulated gene expression, status maintaining, rapid intracellular calcium increase, phosphorylation of MET1 cofactor Taiman, high affinity III. Gene knockout Cad96ca Fgfr1 CRISPR/Cas9 embryo knockdown various cells, overexpression CAD96CA FGFR1 mammalian HEK-293T cells all supported transmitting signal receptors.
Language: Английский
Citations
0
Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Language: Английский
Citations
0
Insect Biochemistry and Molecular Biology, Journal Year: 2022, Volume and Issue: 142, P. 103727 - 103727
Published: Jan. 29, 2022
Language: Английский
Citations
16