The transcription factor Capicua maintains the oocyte polarity in the panoistic ovary of the German cockroach.

Developmental Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

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A piRNA regulating oogenesis and embryo development in cockroaches

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 12, 2024

Abstract The PIWI-interacting RNAs (piRNAs) are small non-coding ranging from 26 to 31 nucleotides. Initially, piRNA functions were associated with repressing transposable elements as part of a mechanism that maintains genomic stability across evolution. However, in the last years, new regulatory for piRNAs have been identified. Now, we investigated role insect oogenesis and embryo development. We studied expression function piRNA-83679, one most abundantly expressed Blattella germanica adult ovaries. piRNA-83679 is germinal somatic cells ovaries during gonadotropic cycle, maternally provided egg. Using an antisense oligonucleotide (ASO), reduced levels B. females, enabling study its functions. depletion affected different ecdysone chorion genes ovary, organization F-actin fibers basal pole follicle ovarian follicle. As result, eggs not correctly encapsulated ootheca. Moreover, delayed oviposition development, especially early stages.

Language: Английский

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Non‐gonadal expression of piRNAs is widespread across Arthropoda

FEBS Letters, Journal Year: 2024, Volume and Issue: 599(1), P. 3 - 18

Published: Oct. 2, 2024

PIWI‐interacting RNAs (piRNAs) were discovered in the early 2000s and became known for their role protecting germline genome against mobile genetic elements. Successively, piRNAs also detected somatic cells of gonads multiple animal species. In recent years, have been reported non‐gonadal tissues various arthropods, contrary to initial assumptions being exclusive gonads. Here, we performed an extensive literature review, which revealed that reports on piRNA expression are not limited a few specific Instead, when studies considered collectively, it appears be widespread phenomenon across arthropods. Furthermore, systematically analyzed 168 publicly available small RNA‐seq datasets from diverse 17 species, further supported bibliographic expressed species Arthropoda.

Language: Английский

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Early Embryonic Development of the German Cockroach Blattella germanica

Published: July 10, 2024

Abstract Background Early embryogenesis is characterized by dramatic cell proliferation and movement. In most insects, early includes a phase called the uniform blastoderm, during which cells evenly cover entirety of egg. However, embryo German cockroach, Blattella germanica , like those many insects within super order Polyneoptera, does not have blastoderm; instead, its first condense rapidly at site future germband. We investigated development in this species to understand how gene expression or conserved these insect embryos with distinct behaviors. Results present detailed time series nuclear division distribution from fertilization through germband formation report patterns for patterning genes hunchback caudal twist polarization mesoderm formation. show course spatial two involved segmentation cascade, hedgehog even-skipped, demonstrate dynamics process. Conclusions Despite differences between blastoderms Polyneopteran more well-studied developmental models, are mostly similar. Genes associated axis determination other activated relatively late probably maternally deposited. The phases – simultaneous sequential might indicate broadly mode morphological differentiation. we here should be value further investigation into causes blastoderm type.

Language: Английский

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Capicua maintains anterior-posterior axis inBlattella germanicaovaries

Published: Oct. 22, 2024

Abstract The establishment of the symmetry axis is crucial for development all organisms. In insects, this process begins early in oogenesis with correct distribution mRNAs and proteins oocyte. One that contribute to organizing Capicua (Cic). Cic has been studied Drosophila melanogaster embryo development, it maternally expressed required establish dorso-ventral axis, acting as a repressor transcription factor. Although sequence conserved across species, its function other types insect ovaries still unknown. We wondered whether insects maintained through evolution despite ovary type or if modified parallel evolution. To address this, we phylogenetically basal insect, cockroach Blattella germanica , species panoistic ovaries. B. shares 80% identity D. Cic. Our findings indicate essential oocyte involved establishing anterior- posterior axis.

Language: Английский

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Hox-determined appendage regeneration restores cockroach courtship rituals

Science China Life Sciences, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 5, 2024

Language: Английский

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