Lactiplantibacillus plantarum and L. paraplantarum encode agr-like loci that interfere with quorum sensing and virulence gene expression in Staphylococcus aureus DOI Creative Commons
Weizhe Wang, Ifigeneia Kyrkou, Martin S. Bojer

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 18, 2024

Abstract The pathogenicity of Staphylococcus aureus is largely regulated by the agr quorum sensing (QS) system encoded agrBDCA, which coordinates virulence factor production through secretion and auto-inducing peptides (AIPs). agr-like systems are present also in coagulase-negative staphylococci, several these encode AIPs that inhibit S. QS. In lactic acid bacteria, a similar locus was previously identified Lactiplantibacillus plantarum WCSF1 termed lamBDCA. Here, we characterized lamBDCA L. LMG 13556 paraplantarum CIRM-BIA 1870, explored effects on Notably, found co-cultivation with significantly inhibits QS hemolysin production, while less so for plantarum. inhibition lost upon disruption its locus, suggesting AIP mediates cross-species interference aureus agr activation. Transcriptomic analysis revealed controls expression genes belonging to various functional categories, including stress response metabolism. latter includes encoding riboflavin (B2 vitamin) biosynthesis, enabled growth paraplantarum lamB mutant presence roseoflavin, toxic analogue. Collectively, our results show 1870 interferes gene suppression, they implicate probiotic properties paraplantarum.

Language: Английский

Characterization of agr-like Loci in Lactiplantibacillus plantarum and L. paraplantarum and Their Role in Quorum Sensing and Virulence Inhibition of Staphylococcus aureus DOI Creative Commons
Weizhe Wang, Ifigeneia Kyrkou, Martin S. Bojer

et al.

Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Abstract The pathogenicity of Staphylococcus aureus is largely regulated by the agr quorum sensing (QS) system encoded agrBDCA , which coordinates virulence factor production through secretion and auto-inducing peptides (AIPs). -like systems are also present in coagulase-negative staphylococci, several these encode AIPs that inhibit S. QS. In lactic acid bacteria, a similar locus was previously identified Lactiplantibacillus plantarum WCSF1 termed lamBDCA . Here, we characterized L. LMG 13556 paraplantarum CIRM-BIA 1870, explored effects on Notably, found co-cultivation with significantly inhibits QS hemolysin production, while less so for inhibition lost upon disruption its locus, suggesting AIP mediates cross-species interference activation. Transcriptomic analysis revealed controls expression genes belonging to various functional categories, including stress response metabolism. latter includes encoding riboflavin (B2 vitamin) biosynthesis, enabled growth lamB mutant presence roseoflavin, toxic analogue. Collectively, our results show 1870 interferes gene suppression, they implicate probiotic properties

Language: Английский

Citations

0
Combination therapy delays antimicrobial resistance after adaptive laboratory evolution of Staphylococcus aureus DOI Creative Commons

Maiken Engelbrecht Petersen,

Amanda Batoul Khamas, Lars Østergaard

et al.

Antimicrobial Agents and Chemotherapy, Journal Year: 2025, Volume and Issue: unknown

Published: March 14, 2025

ABSTRACT Antibiotic resistance, driven by misuse and overuse of antibiotics, is one the greatest threats against human health. The antimicrobial pressure during prolonged antibiotic treatment chronic bacterial infections selects for resistance. While combinations may reduce resistance emergence, antibiotic-tolerant persister cells can serve as a reservoir development. Therefore, targeting these with anti-persister drugs might provide novel strategy prevention. In this study, we conducted 42 days adaptive laboratory evolution using Staphylococcus aureus exposed to rifampicin, ciprofloxacin, daptomycin, vancomycin, alone or in combination drug mitomycin C. We monitored susceptibility daily assessed phenotypic changes growth biofilm formation evolved strains. Whole-genome sequencing revealed mutations linked shifts. Rifampicin developed within few days, while ciprofloxacin daptomycin emerged approximately 3 weeks. Treatments vancomycin C resulted minimal susceptibility. therapy delayed it did not fully prevent it. Notably, rifampicin maintained throughout long-term experiment. Sub-inhibitory treatments selected both previously characterized mutations, including unprecedented alterations nucleotide excision repair system azoreductase following exposure. development observed therapy, particularly C’s ability suppress suggests potential therapeutic applications. Future studies should evaluate clinical efficacy preventing across different pathogens infection models.

Language: Английский

Citations

0
Impact of emodin alone or in combination with ampicillin on methicillin-resistant Staphylococcus aureus biofilms in vitro DOI

Maoying Zhao,

Fuhong Chen,

Wei Yang

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

Abstract Methicillin-resistant Staphylococcus aureus (MRSA) is recognized as a significant global health concern. The development of resistance to broad spectrum antibiotics, particularly following biofilm formation, renders conventional therapeutic options for MRSA ineffective. Three clinical isolates were examined in vitro assess their biofilm-forming capacity and the disruptive effects on pre-established (via crystal violet staining scanning electron microscopy), quantify extracellular DNA (eDNA) release after exposed emodin alone or combination with ampicillin. In addition, real-time PCR was employed investigate impact expression biofilm-related genes biofilms. inhibitory effect formation disruption observed dose dependent manner. antagonistic activity ampicillin against biofilms confirmed through adhesion assays. Real-time analysis revealed that emodin, either ampicillin, effectively downregulated transcriptional levels fnbpB, clfA atlA, but not icaA. drug treatment resulted reduction eDNA protein contain EPS (extracellular polymeric substances), which corresponded markedly decreased transcript level atlA respectively. These observations suggest holds potential approach infections.

Language: Английский

Citations

0
Staphylococcus aureus: A Review of the Pathogenesis and Virulence Mechanisms DOI Creative Commons

Rahima Touaitia,

Assia Mairi, Nasir A. Ibrahim

et al.

Antibiotics, Journal Year: 2025, Volume and Issue: 14(5), P. 470 - 470

Published: May 6, 2025

Staphylococcus aureus is a formidable human pathogen responsible for infections ranging from superficial skin lesions to life-threatening systemic diseases. This review synthesizes current knowledge on its pathogenesis, emphasizing colonization dynamics, virulence mechanisms, biofilm formation, and antibiotic resistance. By analyzing studies PubMed, Scopus, Web of Science, we highlight the pathogen’s adaptability, driven by surface adhesins (e.g., ClfB, SasG), secreted toxins PVL, TSST-1), metabolic flexibility in iron acquisition amino acid utilization. Nasal, skin, oropharyngeal are reservoirs invasive infections, with persistence horizontal gene transfer exacerbating antimicrobial resistance, particularly methicillin-resistant S. (MRSA). The underscores clinical challenges multidrug-resistant strains, including vancomycin resistance decolonization strategies’ failure target single anatomical sites. Key discussions address host–microbiome interactions, immune evasion tactics, limitations therapies. Future directions advocate novel anti-virulence therapies, multi-epitope vaccines, AI-driven diagnostics combat evolving Strengthening global surveillance interdisciplinary collaboration critical mitigating public health burden aureus.

Language: Английский

Citations

0
Mitomycin C Retains Efficacy after Adaptive Laboratory Evolution of Staphylococcus aureus DOI Creative Commons

Maiken Engelbrecht Petersen,

Amanda Batoul Khamas, Lars Østergaard

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 17, 2024

Abstract Antibiotic resistance is one of the greatest threats against human health and misuse overuse antibiotics a key factor driving development. During prolonged antibiotic treatment chronic infections, antimicrobial pressure facilitates selection mutations. It has been suggested that using in combinations may reduce emergence resistance. Furthermore, tolerant persister cells be reservoir for development, so targeting with anti-persister drugs could also In this study, we conducted 42-day adaptive laboratory evolution experiment Staphylococcus aureus exposed to common drug mitomycin C, either alone or combination. We monitored susceptibility daily assessed phenotypic changes growth biofilm formation evolved strains. Whole-genome sequencing revealed mutations linked shifts. Resistance developed rapidly rifampicin, while ciprofloxacin daptomycin showed slower emergence. Treatments vancomycin C resulted minimal susceptibility. Combination therapies generally delayed resistance, though was not fully prevented. Notably, combined rifampicin effectively suppressed Sub-inhibitory concentrations were associated both known novel mutations, including nucleotide excision repair system azoreductase, following treatment—mutations previously reported. While combination therapy C’s efficacy ability prevent highlights its potential combating Further investigation needed evaluate broader application prevention.

Language: Английский

Citations

0
Lactiplantibacillus plantarum and L. paraplantarum encode agr-like loci that interfere with quorum sensing and virulence gene expression in Staphylococcus aureus DOI Creative Commons
Weizhe Wang, Ifigeneia Kyrkou, Martin S. Bojer

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 18, 2024

Abstract The pathogenicity of Staphylococcus aureus is largely regulated by the agr quorum sensing (QS) system encoded agrBDCA, which coordinates virulence factor production through secretion and auto-inducing peptides (AIPs). agr-like systems are present also in coagulase-negative staphylococci, several these encode AIPs that inhibit S. QS. In lactic acid bacteria, a similar locus was previously identified Lactiplantibacillus plantarum WCSF1 termed lamBDCA. Here, we characterized lamBDCA L. LMG 13556 paraplantarum CIRM-BIA 1870, explored effects on Notably, found co-cultivation with significantly inhibits QS hemolysin production, while less so for plantarum. inhibition lost upon disruption its locus, suggesting AIP mediates cross-species interference aureus agr activation. Transcriptomic analysis revealed controls expression genes belonging to various functional categories, including stress response metabolism. latter includes encoding riboflavin (B2 vitamin) biosynthesis, enabled growth paraplantarum lamB mutant presence roseoflavin, toxic analogue. Collectively, our results show 1870 interferes gene suppression, they implicate probiotic properties paraplantarum.

Language: Английский

Citations

0