Analysis of Stratifin Expression and Proteome Variation in a Rat Model of Acute Lung Injury DOI Creative Commons
Ayaka Yoshida, Yoshiaki Hashimoto,

Hirotoshi Akane

et al.

Journal of Proteome Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 28, 2025

Diffuse alveolar damage (DAD) is a pathological hallmark of severe interstitial lung diseases, such as acute respiratory distress syndrome (ARDS), and linked to poor prognosis. Previously, we identified 14–3–3σ/stratifin (SFN) serum biomarker candidate for diagnosing DAD. To clarify the time-dependent relationship between SFN expression DAD, here investigated molecular changes in serum, bronchoalveolar lavage fluid (BALF), tissue an oleic acid (OA)-induced ARDS rat model. Acute edema was observed after OA administration, followed by epithelial cell proliferation increased BALF levels. Proteomic analysis extracts revealed that proteins related "inflammatory response" "HIF-1 signaling," including plasminogen activator inhibitor-1, were markedly 3 h injury, gradual decrease. Conversely, associated with "cell cycle" "p53 pathway," SFN, showed persistent increase starting at peaking 48 h. Western blotting immunohistochemistry confirmed expressed part proliferated type-II cells, accompanied p53 activation, important event differentiation into type-I cells. may be closely remodeling during repair process injury.

Language: Английский

The Contentious Origins of SARS-CoV-2: A Comprehensive Review of Current Knowledge DOI Creative Commons
José L. Domingo

Published: Feb. 11, 2025

The emergence of SARS-CoV-2 in late 2019 triggered a global pandemic (COVID-19) that has claimed millions lives and continues to impact public health systems worldwide. This review examines current scientific evidence regarding the virus's origins, focusing on two primary hypotheses: zoonotic spillover laboratory-related emergence. A comprehensive analysis peer-reviewed literature published between December 2023 January 2025 was conducted, identifying relevant studies through searches PubMed Scopus databases. revealed strong phylogenetic supporting natural origin, with several indicating close genetic relationships bat coronaviruses. Environmental sampling data from Huanan Seafood Market detected RNA high number environmental samples animal stalls. While preponderance supports spillover, absence definitively identified intermediate host maintains some uncertainty. concludes evidence-based recommendations for enhanced viral surveillance, implementation One Health approaches, strengthened international collaboration frameworks prevent future coronavirus pandemics.

Language: Английский

Citations

0
The Contentious Origins of SARS-CoV-2: A Comprehensive Review of Current Knowledge DOI Creative Commons
José L. Domingo

Published: Feb. 17, 2025

The emergence of SARS-CoV-2 in late 2019 triggered a global pandemic (COVID-19) that has claimed millions lives and continues to impact public health systems worldwide. This review examines current scientific evidence regarding the virus's origins, focusing on two primary hypotheses: zoonotic spillover laboratory-related emergence. A comprehensive analysis peer-reviewed literature primarily published between December 2023 January 2025 was conducted, identifying relevant studies through searches PubMed Scopus databases. Key earlier publications are also included provide essential background context. revealed strong phylogenetic supporting natural origin, with several indicating close genetic relationships bat coronaviruses. Environmental sampling data from Huanan Seafood Market detected RNA high number environmental samples animal stalls. While preponderance supports spillover, absence definitively identified intermediate host maintains some uncertainty. concludes evidence-based recommendations for enhanced viral surveillance, implementation One Health approaches, strengthened international collaboration frameworks prevent future coronavirus pandemics.

Language: Английский

Citations

0
The Contentious Origins of SARS-CoV-2: A Comprehensive Review of Current Knowledge DOI Creative Commons
José L. Domingo

Published: Feb. 19, 2025

The emergence of SARS-CoV-2 in late 2019 triggered a global pandemic (COVID-19) that has claimed millions lives and continues to impact public health systems worldwide. This review examines current scientific evidence regarding the virus's origins, focusing on two primary hypotheses: zoonotic spillover laboratory-related emergence. A comprehensive analysis peer-reviewed literature primarily published between December 2023 January 2025 was conducted, identifying relevant studies through searches PubMed Scopus databases. Key earlier publications are also included provide essential background context. revealed strong phylogenetic supporting natural origin, with several indicating close genetic relationships bat coronaviruses. Environmental sampling data from Huanan Seafood Market detected RNA high number environmental samples animal stalls. While preponderance supports spillover, absence definitively identified intermediate host maintains some uncertainty. concludes evidence-based recommendations for enhanced viral surveillance, implementation One Health approaches, strengthened international collaboration frameworks prevent future coronavirus pandemics.

Language: Английский

Citations

0
Analysis of Stratifin Expression and Proteome Variation in a Rat Model of Acute Lung Injury DOI Creative Commons
Ayaka Yoshida, Yoshiaki Hashimoto,

Hirotoshi Akane

et al.

Journal of Proteome Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 28, 2025

Diffuse alveolar damage (DAD) is a pathological hallmark of severe interstitial lung diseases, such as acute respiratory distress syndrome (ARDS), and linked to poor prognosis. Previously, we identified 14–3–3σ/stratifin (SFN) serum biomarker candidate for diagnosing DAD. To clarify the time-dependent relationship between SFN expression DAD, here investigated molecular changes in serum, bronchoalveolar lavage fluid (BALF), tissue an oleic acid (OA)-induced ARDS rat model. Acute edema was observed after OA administration, followed by epithelial cell proliferation increased BALF levels. Proteomic analysis extracts revealed that proteins related "inflammatory response" "HIF-1 signaling," including plasminogen activator inhibitor-1, were markedly 3 h injury, gradual decrease. Conversely, associated with "cell cycle" "p53 pathway," SFN, showed persistent increase starting at peaking 48 h. Western blotting immunohistochemistry confirmed expressed part proliferated type-II cells, accompanied p53 activation, important event differentiation into type-I cells. may be closely remodeling during repair process injury.

Language: Английский

Citations

0