Recent Advances in CRISPR/Cas System-Based Biosensors for the Detection of Foodborne Pathogenic Microorganisms DOI Creative Commons

Sanlei Xie,

Yuehong Yue,

Fan Yang

et al.

Micromachines, Journal Year: 2024, Volume and Issue: 15(11), P. 1329 - 1329

Published: Oct. 30, 2024

Foodborne pathogens pose significant risks to food safety. Conventional biochemical detection techniques are facing a series of challenges. In recent years, with the gradual development CRISPR (clustered regularly interspaced short palindromic repeats) technology, CRISPR/Cas system-based biosensors, newly emerging have received much attention from researchers because their supreme flexibility, sensitivity, and specificity. While numerous CRISPR-based biosensors broad application in field environmental monitoring, safety, point-of-care diagnosis, they remain high demand summarize advances for foodborne pathogen detection. this paper, we briefly classify discuss working principles systems trans-cleavage activity applications pathogenic microorganisms. We highlight current status, unique feature each system biosensing platforms, integration CRISPR-Cas other techniques, concluding discussion advantages, disadvantages, future directions.

Language: Английский

An overview of biosensor advancements for detecting botulinum neurotoxins: addressing food safety and biowarfare risks DOI

Ashok Saini,

Neelam Yadav, Bijender Singh

et al.

Analytical Biochemistry, Journal Year: 2025, Volume and Issue: 701, P. 115801 - 115801

Published: Feb. 5, 2025

Language: Английский

Citations

1
Recent Advances in Biosensor Technologies for Meat Production Chain DOI Creative Commons
Ivan Nastasijević, Ivana Kundačina, Stefan Jarić

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(5), P. 744 - 744

Published: Feb. 22, 2025

Biosensors are innovative and cost-effective analytical devices that integrate biological recognition elements (bioreceptors) with transducers to detect specific substances (biomolecules), providing a high sensitivity specificity for the rapid accurate point-of-care (POC) quantitative detection of selected biomolecules. In meat production chain, their application has gained attention due increasing demand enhanced food safety, quality assurance, fraud detection, regulatory compliance. can foodborne pathogens (Salmonella, Campylobacter, Shiga-toxin-producing E. coli/STEC, L. monocytogenes, etc.), spoilage bacteria indicators, contaminants (pesticides, dioxins, mycotoxins), antibiotics, antimicrobial resistance genes, hormones (growth promoters stress hormones), metabolites (acute-phase proteins as inflammation markers) at different modules along from livestock farming packaging in farm-to-fork (F2F) continuum. By real-time data biosensors enable early interventions, reducing health risks (foodborne outbreaks) associated contaminated meat/meat products or sub-standard products. Recent advancements micro- nanotechnology, microfluidics, wireless communication have further sensitivity, specificity, portability, automation biosensors, making them suitable on-site field applications. The integration blockchain Internet Things (IoT) systems allows acquired management, while artificial intelligence (AI) machine learning (ML) enables processing, analytics, input risk assessment by competent authorities. This promotes transparency traceability within fostering consumer trust industry accountability. Despite biosensors' promising potential, challenges such scalability, reliability complexity matrices, approval still main challenges. review provides broad overview most relevant aspects current state-of-the-art development, challenges, opportunities prospective applications regular use safety monitoring, clarifying perspectives.

Language: Английский

Citations

0
Nitrogen-Doped Graphene Quantum Dots (N-GQDs): A Promising Material for the Development of Electrochemical Immunosensors DOI Creative Commons
Gustavo Martins,

Ana Laura S. Galvan,

Márcia Gabriela Pianaro Valenga

et al.

ACS Applied Nano Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Language: Английский

Citations

0
Innovations in paper-based analytical devices and portable absorption photometers for onsite analysis DOI Open Access
Sasikarn Seetasang, Mika I. Umeda, Jianchao Ren

et al.

Analytical Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

Language: Английский

Citations

0
Toward safer and sustainable food preservation: a comprehensive review of bacteriocins in the food industry DOI Open Access
José Carlos Parada-Fabián, Ana Karen Álvarez-Contreras, Iván Natividad-Bonifacio

et al.

Bioscience Reports, Journal Year: 2025, Volume and Issue: 45(04), P. 277 - 302

Published: April 1, 2025

Bacteriocins are considered promising natural biopreservatives in the food industry because of their broad spectrum antimicrobial activity against Gram-positive bacteria and foodborne pathogens. This review provides information on several bacteriocins (nisin, pediocin, Micocin®, lacticin 3147, enterocin AS-48), mechanisms action, applications, discussion regulatory requirements for approval as additives by Food Drug Administration (FDA) European Union to improve safety. Nisin (the most studied bacteriocin), recognized generally regarded safe FDA, is used a preservative. Pediocin, derived from Pediococcus acidilactici, shows efficacy Listeria species vegetable meat products. mixture produced Carnobacterium maltaromaticum CB1, effective Clostridium botulinum monocytogenes. Lacticin composed two peptides: Ltnα Ltnβ, synergistic antibacterial with potential applications control pathogens dairy Enterococcin AS-48, Enterococcus faecalis subsp. liquefaciens S-48, exhibits broad-spectrum has been biopreservation number For approval, following criteria must be met: determination identity, chemical composition, safety assessments, recommended concentrations use. Despite difficulties posed large-scale production purification, hold enormous improving shelf life; however, further research required harness future preservation strategies.

Language: Английский

Citations

0
Recent Advances in CRISPR/Cas System-Based Biosensors for the Detection of Foodborne Pathogenic Microorganisms DOI Creative Commons

Sanlei Xie,

Yuehong Yue,

Fan Yang

et al.

Micromachines, Journal Year: 2024, Volume and Issue: 15(11), P. 1329 - 1329

Published: Oct. 30, 2024

Foodborne pathogens pose significant risks to food safety. Conventional biochemical detection techniques are facing a series of challenges. In recent years, with the gradual development CRISPR (clustered regularly interspaced short palindromic repeats) technology, CRISPR/Cas system-based biosensors, newly emerging have received much attention from researchers because their supreme flexibility, sensitivity, and specificity. While numerous CRISPR-based biosensors broad application in field environmental monitoring, safety, point-of-care diagnosis, they remain high demand summarize advances for foodborne pathogen detection. this paper, we briefly classify discuss working principles systems trans-cleavage activity applications pathogenic microorganisms. We highlight current status, unique feature each system biosensing platforms, integration CRISPR-Cas other techniques, concluding discussion advantages, disadvantages, future directions.

Language: Английский

Citations

3