Storage and transport of labile iron is mediated by lysosomes in axons and dendrites of hippocampal neurons DOI Open Access

Aiyarin Kittilukkana,

Asunción Carmona, Andréa Somogyi

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 11, 2024

Abstract Iron dyshomeostasis in neurons, involving iron accumulation and abnormal redox balance, is implicated neurodegeneration. In particular, labile iron, a highly reactive pool of intracellular plays prominent role iron-induced neurological damage. However, the mechanisms governing detoxification transport within neurons are not fully understood. This study investigates storage ferrous Fe(II) cultured primary rat hippocampal neurons. distribution was studied using live cell confocal microscopy with selective fluorescent dye, synchrotron X-ray fluorescence (SXRF) for total distribution. Fluorescent labelling axon initial segment lysosomes allowed to be correlated these subcellular compartments. The results show that stored somas, axons dendrites lysosomal transported retrogradely anterogradely along dendrites. addition, we have developed methodological workflow quantify relative neurites. method based on correlative imaging combined quantitative elemental mapping by SXRF. Quantitative analysis revealed after exposure, accounts small but significant percentage content These result suggest exposure Fe(II), mainly present non-reactive form while smaller fraction can axons.

Language: Английский

Storage and transport of labile iron is mediated by lysosomes in axons and dendrites of hippocampal neurons DOI Open Access

Aiyarin Kittilukkana,

Asunción Carmona, Andréa Somogyi

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 11, 2024

Abstract Iron dyshomeostasis in neurons, involving iron accumulation and abnormal redox balance, is implicated neurodegeneration. In particular, labile iron, a highly reactive pool of intracellular plays prominent role iron-induced neurological damage. However, the mechanisms governing detoxification transport within neurons are not fully understood. This study investigates storage ferrous Fe(II) cultured primary rat hippocampal neurons. distribution was studied using live cell confocal microscopy with selective fluorescent dye, synchrotron X-ray fluorescence (SXRF) for total distribution. Fluorescent labelling axon initial segment lysosomes allowed to be correlated these subcellular compartments. The results show that stored somas, axons dendrites lysosomal transported retrogradely anterogradely along dendrites. addition, we have developed methodological workflow quantify relative neurites. method based on correlative imaging combined quantitative elemental mapping by SXRF. Quantitative analysis revealed after exposure, accounts small but significant percentage content These result suggest exposure Fe(II), mainly present non-reactive form while smaller fraction can axons.

Language: Английский

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