Immunoregulatory Effects of Codonopsis pilosula Polysaccharide Modified Selenium Nanoparticles on H22 Tumor-Bearing Mice DOI Creative Commons
Yan Long, Hongfei Ji,

Jiajing Yang

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(24), P. 4073 - 4073

Published: Dec. 17, 2024

Codonopsis pilosula polysaccharide (CPP) and rare element selenium (Se) have been proved to exert various biological activities, our previous study demonstrated that nanoparticles modified with CPP (CPP-SeNPs) possessed significantly enhanced tumor cytotoxicity in vitro. This aimed investigated the inhibitory effects of CPP-SeNPs complex on H22 solid tumors via immune enhancement. In this study, tumor-bearing mice model was constructed, potential mechanisms antitumor were further explored by evaluating cytokines expression levels, cells activities apoptotic indicators each group. The results effectively exerted dose-dependent protective organs vivo, leading increase peripheral white blood cell counts inhibition growth rate 47.18% high-dose group (1.5 mg/kg). Furthermore, treatment elevated levels TNF-α, IFN-γ, IL-2 sera, NK cytotoxicity, augmented macrophage phagocytosis capacity, as well increased both amounts proliferation activity lymphocyte subsets. improved system’s ability clear up-regulating Bax while down-regulating Bcl-2 within tumors, indicating activation mitochondrial apoptosis pathway. Therefore, administration can inhibit enhancing response mice, which might be relevant regulation gut microbiota short-chain fatty acids metabolisms. These findings could provide theoretical support data foundation for development functional food drug adjuvants.

Language: Английский

Study on the Effect of Radish Sprouts on Short-Chain Fatty Acids and Gut Microbial Diversity in Healthy Individuals DOI Creative Commons
Ru Li, Xuehong Chen, Cong Shi

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(2), P. 170 - 170

Published: Jan. 8, 2025

This study aimed to assess the impact of radish sprouts on gut microbiota healthy individuals. Radish sprout additives, subjected short-term storage and steam treatment, were used intervene in an vitro culture human microbiota. The influence was evaluated by monitoring short-chain fatty acid (SCFA) content proportion fermentation broth, microbial diversity assessed using 16S rDNA amplicon sequencing. results indicated that produced a substantial amount SCFA within 48 h fermentation, with right-skewed distribution across all groups. addition both digestates enhanced Firmicutes diversity, while Bacteroidetes Proteobacteria remained stable between control fresh 30 s treatment group showed increase decrease diversity. abundance Bacilli, Bacillaceae, Bacillus significantly higher steam-treated groups compared control. Both enriched showing superior effects. These findings suggest consumption may positively microbiota, potentially enhancing these benefits.

Language: Английский

Citations

1
The Malignant Transformation of Viral Hepatitis to Hepatocellular Carcinoma: Mechanisms and Interventions DOI Creative Commons
Huimin Yuan, Ruochen Xu, Senlin Li

et al.

MedComm, Journal Year: 2025, Volume and Issue: 6(3)

Published: March 1, 2025

Hepatocellular carcinoma (HCC) is a leading cause of cancer-related mortality globally, predominantly associated with chronic hepatitis B virus (HBV) and C (HCV) infections. These infections drive persistent liver inflammation, culminating in cellular dysregulation, fibrosis, cancer. Despite advancements targeted therapies, drug resistance the lack reliable biomarkers for patient stratification still terribly hinder treatment viral HCC. To this end, review delves into intricate mechanisms underlying malignant transformation to HCC, including integration, genomic instability, epigenetic modifications, oxidative stress, gut microbiota dysbiosis, immune escape, abnormal signaling pathways, highlighting their complex interactions synergies. Cutting-edge preclinical clinical HCC management, lifestyle immunotherapies, gene-based approaches, innovative treatments, are further investigated, particular priority given therapeutic potential future applications overcoming current limitations. By synthesizing recent scientific insights, aims deepen understanding pathogenesis context hepatitis, paving way novel targets personalized strategies, ultimately improving outcomes.

Language: Английский

Citations

1
A Pilot Study on Qualitative Metabolomics to Characterize Lewis Lung Carcinoma in Mice DOI Creative Commons
Agnieszka Stawarska, Magdalena Bamburowicz‐Klimkowska, Dariusz Maciej Pisklak

et al.

Life, Journal Year: 2025, Volume and Issue: 15(2), P. 202 - 202

Published: Jan. 29, 2025

Metabolomics is a powerful tool that can be used to identify different stages in cancer development. In this study, the metabolomic profile of Lewis lung carcinoma (LLC) was characterized C57BL/6 mice bearing LLC tumors. Magnetic resonance spectroscopy (nuclear magnetic resonance—NMR) applied using 400 MHz 1H NMR spectrometer. Two types metabolites (polar and non-polar) were identified on based analysis methanol/water chloroform extracts collected from samples mice. The investigated metabolomics show neoplastic processes growing may affect carbohydrate; alanine glutamate; leucine isoleucine; lysine; creatine; choline metabolism, whereas hypoxia states due elevated lactate tissues. could considered valuable biomarker translational research.

Language: Английский

Citations

0
Anticancer Potential of Prebiotics: Targeting Estrogen Receptors and PI3K/AKT/mTOR in Breast Cancer DOI Creative Commons
Hussein Sabit,

Sama Abouelnour,

Bassel M. Hassen

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(4), P. 990 - 990

Published: April 18, 2025

Estrogen receptors (ERs) play a critical role in breast cancer (BC) development and progression, with ERα being oncogenic ERβ exhibiting tumor-suppressive properties. The interaction between ER signaling other molecular pathways, such as PI3K/AKT/mTOR, influences tumor growth endocrine resistance. Emerging research highlights the of prebiotics modulating gut microbiota, which may influence estrogen metabolism, immune function, therapeutic responses BC. This review explores impact on receptor modulation, microbiota composition, regulation, metabolic pathways cancer. potential adjunctive therapies to enhance treatment efficacy mitigate chemotherapy-related side effects is discussed. A comprehensive analysis recent preclinical clinical studies was conducted, examining reprogramming short-chain fatty acids (SCFAs) derived from prebiotic fermentation epigenetic regulation resistance also evaluated. Prebiotics were found modulate microbiota-estrogen axis, reduce inflammation, responses. SCFAs demonstrated selective downregulation reprogramming, suppressing growth. Synbiotic interventions effects, improving quality life patients. offer promising avenue for prevention therapy by pathways. Future trials are needed validate their treatments management.

Language: Английский

Citations

0
Long-Term Exposure to Microplastics Promotes Early-Stage Hepatocarcinogenesis Induced by Diethylnitrosamine in Rats by Modulation of Their Gut Microbiota DOI Creative Commons

Huina Guo,

Jianan Wang,

Shaowen Huang

et al.

Toxics, Journal Year: 2025, Volume and Issue: 13(5), P. 353 - 353

Published: April 29, 2025

Hepatocarcinogenesis is linked to environmental factors, with microplastics (MPs) emerging as a global concern that may contribute liver injury. However, the impact of MPs on early stages hepatocarcinogenesis has been largely ignored. Here we investigated long-term MP exposure formation preneoplastic lesions during induced by diethylnitrosamine (DEN) in rats. Rats were injected DEN induce lesions, and then they orally administered 1 µm 0.5 mg/kg body weight per day for 20 weeks. The results revealed did not glutathione S-transferase placental form (GST-P)-positive foci these animals, thereby indicating non-carcinogenicity. resulted 1-fold increase both number size GST-P-positive rats initiated compared those treated alone. Accordingly, led 0.61-fold index proliferating cell nuclear antigen (PCNA)-positive cells DEN-initiated when treatment In addition, composition gut microbiota was significantly altered, accompanied various levels short-chain fatty acids. Our suggest can promote pre-neoplastic lesion DEN-induced increased proliferation well alterations acid levels. This highlights potential health risks associated MPs.

Language: Английский

Citations

0
The effects of a modified Mediterranean diet on gut microbiota and clinical outcomes in patients with metastatic colorectal cancer undergoing first-line chemotherapy with either anti-EGFR or anti-VEGF agent: protocol for a randomized pilot study (Preprint) DOI Creative Commons

Salvatore Artale,

Francesca Filiali,

Elena Beretta

et al.

Published: Feb. 21, 2025

BACKGROUND the gut microbiota is attracting increasing interest as an initiator of colorectal cancer. It has been observed that microbial imbalance in and cancer tissue facilitated by a Western type diet, rich meat, sugars, refined grains, while Mediterranean low saturated fat fibers, promotes eubiosis, results reduced risk developing OBJECTIVE to analyze compare patients with metastatic undergoing first-line chemotherapy +/- biological agent (anti-EGFR or anti-VEGF), receiving either free standard modified diet. METHODS this pilot non-drug, interventional prospective, randomized, controlled, single-center, open-label trial. Patients (n=40) will be randomized 1:1 diet Blood fecal samples collected at baseline control visits, for metagenomic metabolomic analysis. The primary endpoint Firmicutes/Bacteroidetes ratio after completion third cycle (time T1). Secondary endpoints are: a) percentage experiencing gastrointestinal side effects T1; b) grade 3/4 c) changes ratio, overall microbiome composition, metabolome T1, sixth (T2) versus baseline. RESULTS trial received ethics approval on July 24th 2024. By January 2025, 7 participants have recruited. study conclude visit T2 last enrolled patient. Results are expected published October 2028. CONCLUSIONS potential provide critical insights into role modifying microbiota, diminishing chemotherapy-related effects, possibly enhancing therapeutic efficacy Additionally, data may pave way future research immunotherapy, potentially influencing both clinical practice public health strategies. CLINICALTRIAL Clinicaltrial.gov NCT06794931 (https://clinicaltrials.gov/search?term=NCT06794931)

Language: Английский

Citations

0
Current status and future perspectives of immunotherapy for abdominal liposarcoma: From basic research to clinical practice DOI Open Access
Aobo Zhuang,

Zhe Xi,

Ying Cheng

et al.

Shijie huaren xiaohua zazhi, Journal Year: 2025, Volume and Issue: 33(2), P. 81 - 88

Published: Feb. 28, 2025

Language: Английский

Citations

0
Immunoregulatory Effects of Codonopsis pilosula Polysaccharide Modified Selenium Nanoparticles on H22 Tumor-Bearing Mice DOI Creative Commons
Yan Long, Hongfei Ji,

Jiajing Yang

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(24), P. 4073 - 4073

Published: Dec. 17, 2024

Codonopsis pilosula polysaccharide (CPP) and rare element selenium (Se) have been proved to exert various biological activities, our previous study demonstrated that nanoparticles modified with CPP (CPP-SeNPs) possessed significantly enhanced tumor cytotoxicity in vitro. This aimed investigated the inhibitory effects of CPP-SeNPs complex on H22 solid tumors via immune enhancement. In this study, tumor-bearing mice model was constructed, potential mechanisms antitumor were further explored by evaluating cytokines expression levels, cells activities apoptotic indicators each group. The results effectively exerted dose-dependent protective organs vivo, leading increase peripheral white blood cell counts inhibition growth rate 47.18% high-dose group (1.5 mg/kg). Furthermore, treatment elevated levels TNF-α, IFN-γ, IL-2 sera, NK cytotoxicity, augmented macrophage phagocytosis capacity, as well increased both amounts proliferation activity lymphocyte subsets. improved system’s ability clear up-regulating Bax while down-regulating Bcl-2 within tumors, indicating activation mitochondrial apoptosis pathway. Therefore, administration can inhibit enhancing response mice, which might be relevant regulation gut microbiota short-chain fatty acids metabolisms. These findings could provide theoretical support data foundation for development functional food drug adjuvants.

Language: Английский

Citations

1