Small Science,
Journal Year:
2024,
Volume and Issue:
4(11)
Published: Aug. 10, 2024
Lateral
flow
immunoassays
(LFIAs)
are
easy-to-use
antigen
tests
that
provide
different
signal
readouts,
with
colorimetric
readouts
being
the
most
commonly
used.
However,
these
analytical
devices
have
relatively
low
sensitivity
and
produce
semiquantitative
results,
limiting
their
diagnostic
applications.
Herein,
we
address
challenges
by
implementing
a
digital
surface-enhanced
Raman
spectroscopy
(SERS)-based
LFIA
for
accurate
ultrasensitive
quantitative
detection
of
SARS-CoV-2
nucleocapsid
(N)
protein.
Compared
average
SERS
intensity
measurements,
approach
allowed
to
overcome
fluctuations
in
scattering
signals,
thereby
increasing
assay.
Our
method
exhibited
quantification
range
viral
protein
nasal
swabs
from
0.001
10
pg
mL-1,
limit
down
1.9
aM
(0.9
fg
mL-1),
improving
LFIAs
conventional-SERS-based
several
orders
magnitude.
Importantly,
this
shows
an
0.03
TCID50
which
is
greater
than
reported
other
immunoassays.
In
conclusion,
successfully
demonstrate
robust
SARS-CoV-2N
at
ultralow
concentrations.
The
improvement
may
pave
way
translate
technology
into
arena
microbes
disease
biomarkers.
Advanced Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 13, 2025
Abstract
Immunochromatographic
assays
(ICAs)
provide
simple
and
rapid
strategies
for
bacterial
diagnosis
but
still
suffer
from
the
problems
of
low
sensitivity
high
dependency
on
paired
antibodies.
Herein,
broad‐spectrum
capture
detection
capability
antibody‐free
electropositive
nanoprobe
are
clarified
bacteria
first
time
an
ultrasensitive
fluorescent
ICA
platform
is
constructed
simultaneous
multiple
pathogens.
A
magnetic
multilayer
quantum
dot
nanocomposite
with
amino‐embedded
SiO
2
shell
(MagMQD@Si
+
)
designed
to
enrich
solutions
effectively,
offer
luminescence,
reduce
background
signals
test
strips,
thus
greatly
improving
stability
technique
pathogen.
The
superior
performance
MagMQD@Si
‐based
through
multiplex
three
common
pathogens
demonstrated,
namely,
Pseudomonas
aeruginosa
,
Streptococcus
pneumoniae
typhi
showing
that
this
possesses
(8–40
cells
mL
−1
),
good
reproducibility
(relative
standard
deviation
<
5.4%),
specificity
target
bacteria.
clinical
utility
proposed
method
verified
30
real
sputum
samples
patients
respiratory
infections,
revealing
has
massive
potential
as
a
powerful
inspection
tool
rapid,
sensitive,
infections.
ACS Nano,
Journal Year:
2024,
Volume and Issue:
18(37), P. 25865 - 25879
Published: Sept. 2, 2024
Greatly
improving
the
sensitivity
and
detection
range
of
lateral
flow
immunoassays
(LFAs)
by
at
least
100
times
without
using
additional
instruments
remains
challenging.
Herein,
we
develop
a
three-dimensional
(3D)
film-like
nanozyme
(GO-Pt
Materials,
Journal Year:
2024,
Volume and Issue:
17(5), P. 1088 - 1088
Published: Feb. 27, 2024
From
1990
to
2024,
this
study
presents
a
groundbreaking
bibliometric
and
sentiment
analysis
of
nanocomposite
literature,
distinguishing
itself
from
existing
reviews
through
its
unique
computational
methodology.
Developed
by
our
research
group,
novel
approach
systematically
investigates
the
evolution
nanocomposites,
focusing
on
microstructural
characterization,
electrical
properties,
mechanical
behaviors.
By
deploying
advanced
Boolean
search
strategies
within
Scopus
database,
we
achieve
meticulous
extraction
in-depth
exploration
thematic
content,
methodological
advancement
in
field.
Our
uniquely
identifies
critical
trends
insights
concerning
microstructure,
attributes,
performance.
The
paper
goes
beyond
traditional
textual
analytics
evaluation,
offering
new
interpretations
data
highlighting
significant
collaborative
efforts
influential
studies
domain.
findings
uncover
language,
shifts,
global
contributions,
providing
distinct
comprehensive
view
dynamic
research.
A
component
is
“State-of-the-Art
Gaps
Extracted
Results
Discussions”
section,
which
delves
into
latest
advancements
This
section
details
various
types
their
properties
introduces
applications,
especially
films.
tracing
historical
progress
identifying
emerging
trends,
emphasizes
significance
collaboration
molding
Moreover,
“Literature
Review
Guided
Artificial
Intelligence”
showcases
an
innovative
AI-guided
research,
first
Focusing
articles
2023,
selected
based
citation
frequency,
method
offers
perspective
interplay
between
nanocomposites
properties.
It
highlights
composition,
structure,
functionality
systems,
integrating
recent
for
overview
current
knowledge.
analysis,
with
average
score
0.638771,
reflects
positive
trend
academic
discourse
increasing
recognition
potential
nanocomposites.
another
novelty,
maps
intellectual
domain,
emphasizing
pivotal
themes
influence
crosslinking
time
attributes.
While
acknowledging
limitations,
exemplifies
indispensable
role
tools
synthesizing
understanding
extensive
body
literature.
work
not
only
elucidates
prevailing
but
also
contributes
insights,
enhancing
Biosensors and Bioelectronics X,
Journal Year:
2024,
Volume and Issue:
17, P. 100457 - 100457
Published: March 6, 2024
Infections
cause
concern
for
mankind,
and
sometimes
their
diagnosis
may
take
much
time.
Owing
to
its
high
sensitivity,
rapidity,
specificity,
the
immunochromatographic
assay
(ICA)
is
used
widely
diagnose
infections.
The
use
of
ICA
this
purpose
makes
it
possible
detect
dangerous
diseases
early,
prevent
progress,
strongly
reduce
treatment
cost
mortality,
increase
life
expectancy.
One
promising
format
involves
gold
nanoparticles.
Here
we
present
principles
history
infections
discuss
prospects
using
nanoparticles
in
ICA.
We
data
on
methods
make
conjugate
effect
particle
size
shape
sensitivity.
bacterial
viral
infections,
review
instrumental
quantifying
results.
Finally,
nanoparticle–based
ACS Applied Nano Materials,
Journal Year:
2024,
Volume and Issue:
7(16), P. 19368 - 19376
Published: Aug. 1, 2024
With
the
increasing
misuse
of
antibiotic
drugs,
convenient
and
ultrasensitive
detection
chloramphenicol
(CAP)
is
essential
for
ensuring
food
safety
human
health.
In
this
study,
a
comprehensive
platform
was
developed
integrating
discrimination
capabilities
by
combining
surface-enhanced
Raman
scattering
(SERS)
with
lateral
flow
immunoassay
(LFIA)
technology,
along
support
vector
machine
(SVM)
model
data
analysis,
to
detect
CAP
residues
in
aquatic
products.
The
CAP-specific
antibody-modified
gold
nanostars
(Au
NSs)
were
synthesized
as
SERS–LFIA
nanoprobes,
achieving
an
ultralow
limit
(LOD)
10
pg/mL
theoretical
LOD
1.6
simulated
samples.
This
sensitivity
surpasses
that
currently
available
commercial
strips
demonstrates
high
specificity.
Additionally,
automated
exhibited
(95.83%)
specificity
(96.43%)
well
accuracy
96%
on
test
set,
marking
8%
improvement
over
traditional
intensity
methods.
simple,
fast,
technique
eliminates
need
sample
pretreatment,
offering
promising
approach
trace
