Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(49), P. 19605 - 19614
Published: Nov. 22, 2024
The
microsomal
epoxide
hydrolase
(mEH)
is
important
in
the
detoxification
of
carcinogens
liver
and
other
tissues
but
also
a
blood
biomarker
hepatitis
cancer.
Improved
analytical
methods
are
needed
for
study
its
role
metabolism
xenobiotics
endogenous
roles
as
diseases.
development
double
nanobody
sandwich
ELISA
offers
significant
improvements
over
traditional
polyclonal
or
monoclonal
antibody-based
assays,
enhancing
both
homogeneity
stability
assay
production.
This
focuses
on
selecting
optimizing
pairs
detecting
human
mEH.
Four
high-affinity
nanobodies
were
identified
tested
thermal
stability.
Combinations
these
evaluated,
revealing
that
MQ4-MQ30
pair
achieved
best
performance
with
limit
detection
(LOD)
1
ng/mL.
Additionally,
polyHRP
was
employed
signal
amplification,
capabilities
despite
challenges
related
to
small
size
single
epitope
recognition
nanobodies.
Comparative
studies
using
microplates
NHS@MF
membranes
performed.
superior
highlighted
their
potential
promising
alternative
point-of-care
testing.
exhibited
high
specificity
mEH
minimal
cross-reactivity
enzymes
effectively
addressed
matrix
effects
plasma
tissue
samples.
These
findings
underscore
ELISAs
reliable
sensitive
detection.
Biosensors,
Journal Year:
2025,
Volume and Issue:
15(3), P. 183 - 183
Published: March 13, 2025
Food
safety
remains
a
significant
global
challenge
that
affects
human
health.
Various
hazards,
including
microbiological
and
chemical
threats,
can
compromise
food
throughout
the
supply
chain.
To
address
issues
ensure
public
health,
it
is
necessary
to
adopt
rapid,
accurate,
highly
specific
detection
methods.
Immunoassays
are
considered
be
an
effective
method
for
of
sensitive
biochemical
indicators
provide
efficient
platform
identification
hazards.
In
immunoassays,
antibodies
function
as
primary
recognition
elements.
Nanobodies
have
potential
valuable
biomolecules
in
diagnostic
applications.
Their
distinctive
physicochemical
structural
characteristics
make
them
excellent
candidates
development
reliable
assays,
promising
alternatives
monoclonal
polyclonal
antibodies.
Herein,
we
summarize
comprehensive
overview
status
prospects
nanobody-based
immunoassays
ensuring
safety.
First,
begin
with
historical
perspective
on
nanobodies
their
unique
characteristics.
Subsequently,
explore
definitions
boundaries
immunosensors,
before
discussing
applications
testing
emerged
over
past
five
years,
follow
different
highlighting
advantages
traditional
Finally,
directions
challenges
discussed.
Due
remarkable
sensitivity,
specificity
versatility,
hold
great
promise
revolutionizing
health
well-being.
Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: June 27, 2024
Abstract
The
detection
of
multiplex
live
pathogens
in
spoilage
aquatic
products
is
critically
important.
In
this
study,
a
dual-mode
assay
for
screening
and
detecting
Escherichia
coli
(E.
coli)
Hafnia
paralvei
(H.
paralvei)
(as
two
typical
products)
based
on
magnetic
poly
(phages)
encoded
probes
(MPEP)
was
developed.
were
prepared
by
grafting
large
number
phages
targeted
different
target
bacteria
long-chain
DNA
structure,
respectively.
They
could
specifically
capture
enrich
E.
H.
separation.
Then,
signal
tags
with
lengths
would
conjugate
the
corresponding
MPEP-bacteria
complex
form
kinds
sandwich
structures,
After
that,
captured
lysed
to
release
both
adenosine
triphosphate
(ATP)
tags.
measurement
includes
steps.
Firstly,
portable
ATP
bioluminescence
meter
employed
rapidly
screen
positive
samples
that
contain
either
bacteria.
Secondly,
only
injected
into
microfluidic
chip
which
detect
various
accurate
quantification
demonstrated
high
sensitivity
(3
CFU/mL
E.coli
5
paralvei),
specificity
(strain
identification),
amplification
(20
folds)
short
time(≤
35min).
It
can
be
applied
other
changing
relative
phage
MPEP.
Furthermore,
proposed
provides
wide
prospect
rapid
determination
foodborne
pathogens.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(49), P. 19605 - 19614
Published: Nov. 22, 2024
The
microsomal
epoxide
hydrolase
(mEH)
is
important
in
the
detoxification
of
carcinogens
liver
and
other
tissues
but
also
a
blood
biomarker
hepatitis
cancer.
Improved
analytical
methods
are
needed
for
study
its
role
metabolism
xenobiotics
endogenous
roles
as
diseases.
development
double
nanobody
sandwich
ELISA
offers
significant
improvements
over
traditional
polyclonal
or
monoclonal
antibody-based
assays,
enhancing
both
homogeneity
stability
assay
production.
This
focuses
on
selecting
optimizing
pairs
detecting
human
mEH.
Four
high-affinity
nanobodies
were
identified
tested
thermal
stability.
Combinations
these
evaluated,
revealing
that
MQ4-MQ30
pair
achieved
best
performance
with
limit
detection
(LOD)
1
ng/mL.
Additionally,
polyHRP
was
employed
signal
amplification,
capabilities
despite
challenges
related
to
small
size
single
epitope
recognition
nanobodies.
Comparative
studies
using
microplates
NHS@MF
membranes
performed.
superior
highlighted
their
potential
promising
alternative
point-of-care
testing.
exhibited
high
specificity
mEH
minimal
cross-reactivity
enzymes
effectively
addressed
matrix
effects
plasma
tissue
samples.
These
findings
underscore
ELISAs
reliable
sensitive
detection.
