Cited by Highly sensitive electrochemical biosensor for MMP-2 detection using multi-stage amplification with PNA T7 RNA polymerase and split CRISPR/Cas12a

N-Doped Porous Carbon Synergistic Freezing-Induced DNA with Catalyzed Hairpin Assembly Enables Electrochemical One-Pot Detection of Pathogen in Food Samples DOI

Y.M. Fan,

Yu Liao,

Zhiying Gao

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 7, 2025

A DNA electrochemical interface biosensor based on screen-printed carbon electrodes (SPCEs) holds promise for point-of-care testing (POCT) detection of pathogens in food safety. Nevertheless, SPCE commonly has a rough surface and suffers from relatively low electron transfer rate, disorder capture probes (CPs), the steric hindrance effect target nucleic acid binding. These issues lead to sensitivity. Herein, simple rapid N-doped porous (NPC)-modified freezing-directed combined with catalyzed hairpin assembly (CHA) was constructed one-pot samples without time-consuming growth cultures. The by modified NPC enhanced properties, CP designed CHA stably fixed electrode high hybridization efficiency. Moreover, signals amplified enable selective sensitive washing steps. This method is wide linear range 101 107 CFU/mL limit 5 Escherichia coli shows specificity against other coexisting pathogens. whole complex performed only within 60 min sample-to-answer, which great potential POCT

Language: Английский

Citations

Hybridization chain reaction and CRISPR/Cas12a-integrated biosensor for precise Ago2 detection DOI

Qiang Tang, Jiayi Zhang, Jianhu Pang

et al.

Bioelectrochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 108975 - 108975

Published: March 1, 2025

Language: Английский

Citations

Progress in biosensing applications of isothermal nucleic acid amplification techniques DOI

C. Q. Feng,

Yuan Liao, Jinxuan Jia

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113919 - 113919

Published: May 1, 2025

Language: Английский

Citations

Magnetic composite microspheres with a controlled mesoporous shell for highly efficient DNA extraction and fragment screening DOI

Xiuli Wang, Qilin Guo, Jia Guo

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(20), P. 4899 - 4908

Published: Jan. 1, 2024

Rapid extraction and screening of high-purity DNA fragments is an indispensable technology in advanced molecular biology. In this article, mesoporous magnetic composite microspheres (MSP@mTiO

Language: Английский

Citations

State-of-the-Art Signal Amplification Strategies for Nucleic Acid and Non-Nucleic Acid Biosensors DOI