Embracing Poisson Encapsulation Statistics for Improved Droplet Digital Immunoassay DOI
Yujuan Chai, Xiaoxiang Hu,

Qi Fang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 18, 2024

Digital immunoassays enable the detection of protein biomarkers with very low concentrations, but analysis stringently requires single-bead encapsulation. Low bead density has been adopted to minimize multiple-bead encapsulations, trade-off is droplet effectiveness (∼10%) in droplet-based assays. Here we report method inclusive digital ELISA (iddELISA) that embraces all types encapsulations by factoring their varied "on-off" probabilities statistical inference. We derived model, optimized encapsulation and immunoreaction, developed an image pipeline for accurate recognition, showing approximately 40% droplets could be used analysis. Using SARS-CoV-2 nucleocapsid as a demonstration, iddELISA achieved limit 0.71 fg/mL, which was much lower than conventional well ELISA. By effectively incorporating multiple simplified immunoassay while improving counting efficiency sensitivity, representing unique concept immunoassays.

Language: Английский

Microfluidic immunoassays for point-of-care testing of SARS-CoV-2 antigens and antibodies DOI

Cuili Li,

Wan Zhou,

Angel Gutierrez Ruiz

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 177, P. 117809 - 117809

Published: June 6, 2024

Language: Английский

Citations

9
Recent advances in point-of-care testing (POCT) system based on upconversion luminescent materials DOI
Shunuo Bian, Tao Jin, Wenqiang Lu

et al.

Materials Science and Engineering B, Journal Year: 2025, Volume and Issue: 314, P. 118049 - 118049

Published: Jan. 27, 2025

Language: Английский

Citations

0
Utilizing Nanomaterials in Microfluidic Devices for Disease Detection and Treatment DOI Creative Commons
Zhenyu Tian, Yatian Fu, Zhaolong Dang

et al.

Nanomaterials, Journal Year: 2025, Volume and Issue: 15(6), P. 434 - 434

Published: March 12, 2025

Microfluidic technology has gained widespread application in the field of biomedical research due to its exceptional sensitivity and high specificity. Particularly when combined with nanomaterials, synergy between two significantly advanced fields such as precision medicine, drug delivery, disease detection, treatment. This article aims provide an overview latest achievements microfluidic nanomaterials detection It delves into applications detecting blood parameters, cardiovascular markers, neurological tumor markers. Special emphasis is placed on their roles treatment, including models vessels, blood–brain barrier, lung chips, tumors. The development emerging medical technologies, particularly skin interactive devices imaging, also introduced. Additionally, challenges future prospects current clinical are discussed. In summary, play indispensable role With continuous advancement technology, will become even more profound extensive.

Language: Английский

Citations

0
Reimagining point-of-care assays: Automated digital microfluidics for multiplex in vitro diagnostics DOI
Fan Wu, Donghao Li, Jingyi Ding

et al.

Talanta, Journal Year: 2025, Volume and Issue: 294, P. 128270 - 128270

Published: May 3, 2025

Language: Английский

Citations

0
Aggregation-induced emission-based competitive immunoassays for “signal-on” detection of proteins with multifunctional metal–organic frameworks as signal tags DOI

Fengli Gao,

Meiling Liu, Weiqiang Wang

et al.

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2024, Volume and Issue: 325, P. 125088 - 125088

Published: Sept. 3, 2024

Language: Английский

Citations

1
Au@Pt@Pd nanozymes based lateral flow immunoassay for quantitative detection of SARS-CoV-2 nucleocapsid protein in nasal swab samples DOI
Chengcheng Li, Jinhui Lu, Chao Xiang

et al.

Microchimica Acta, Journal Year: 2024, Volume and Issue: 191(12)

Published: Nov. 7, 2024

Language: Английский

Citations

1
Generation, Manipulation, Detection and Biomedical Applications of Magnetic Droplets in Microfluidic Chips DOI
Chenyang Xu, Huanhuan Shi,

Zhongjian Tan

et al.

The Analyst, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Microfluidic systems incorporating magnetic droplets have emerged as a focal point of significant interest within the biomedical domain. The allure these lies in their capacity to offer precise control, enable contactless operation, and accommodate minimal sample concentration requirements. Such remarkable features serve mitigate errors arising from human operation other factors during cell or molecular detection. By providing innovative solutions for diagnostics immunoassay applications, droplet microfluidics enhance accuracy efficiency procedures. This review undertakes comprehensive examination research progress microfluidic centered around droplets. It adheres sequential presentation approach, commencing fundamental principles, specifically generation on chip, proceeding transmission mixing microchannel

Language: Английский

Citations

0
Novel microfluidic chips integrated with smart devices for in-situ detection of foodborne pathogenic bacteria DOI

Ruibin Hu,

Wen Wang, Qingfeng Zheng

et al.

Current Opinion in Food Science, Journal Year: 2024, Volume and Issue: unknown, P. 101269 - 101269

Published: Dec. 1, 2024

Language: Английский

Citations

0
Discovery of Fluorescent Naturally‐Occurring Inhibitor of SARS‐CoV‐2 Main Protease by AIE Fluorescent Probe DOI
Hao Lin, Chong‐Jing Zhang

ChemMedChem, Journal Year: 2024, Volume and Issue: 19(21)

Published: July 8, 2024

Abstract Target‐based high‐throughput screening (HTS) is an efficient way to identify potent drugs. However, the accuracy of HTS could be affected by Pan‐Assay Interference Compounds (PAINS). One reason for generation PAINS that inherent photophysical property screened compounds interfere with typically used assay signals including absorption and fluorescence. Our previous studies indicate fluorescent probe based on fluorophore characteristics aggregation‐induced emission (AIE) provide high HTS, especially natural products. Herein, we report AIE‐based main protease (M pro ) severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). We designed synthesized AIE ZLHG5, which has a site can specifically cleaved M produce light‐up Thanks large Stokes shift (~200 nm), effectively inhibitors. After library products obtained two coumarin‐originated inhibitory activity towards protease. This study provides both useful inhibitors

Language: Английский

Citations

0
Embracing Poisson Encapsulation Statistics for Improved Droplet Digital Immunoassay DOI
Yujuan Chai, Xiaoxiang Hu,

Qi Fang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 18, 2024

Digital immunoassays enable the detection of protein biomarkers with very low concentrations, but analysis stringently requires single-bead encapsulation. Low bead density has been adopted to minimize multiple-bead encapsulations, trade-off is droplet effectiveness (∼10%) in droplet-based assays. Here we report method inclusive digital ELISA (iddELISA) that embraces all types encapsulations by factoring their varied "on-off" probabilities statistical inference. We derived model, optimized encapsulation and immunoreaction, developed an image pipeline for accurate recognition, showing approximately 40% droplets could be used analysis. Using SARS-CoV-2 nucleocapsid as a demonstration, iddELISA achieved limit 0.71 fg/mL, which was much lower than conventional well ELISA. By effectively incorporating multiple simplified immunoassay while improving counting efficiency sensitivity, representing unique concept immunoassays.

Language: Английский

Citations

0