ACS Applied Nano Materials, Journal Year: 2024, Volume and Issue: 7(11), P. 13636 - 13648
Published: May 27, 2024
The screening method for α-glucosidase (α-Glu) inhibitors plays a crucial role in the development of antidiabetic drugs. This study introduces cerium-based metal–organic framework (Ce-MOF) nanocrystals highly sensitive colorimetric detection α-Glu inhibitors. synthesized Ce-MOF exhibited excellent oxidase-like activity, efficiently catalyzing oxidation 3,3′,5,5′-tetramethylbenzidine (TMB) to generate blue product (oxTMB) with strong absorption peak at 652 nm, enabling quantitative analysis using spectroscopic techniques. Notably, hydroquinone (HQ) can reduce oxTMB colorless TMB. Specifically, hydrolyze α-arbutin HQ, which subsequently reduces oxidase activity Ce-MOF. However, addition system limited production thereby maintaining stability Leveraging this mechanism, was developed. overcomes limitations conventional p-nitrophenyl-α-d-glucopyranoside (p-NPG) assay and effectively avoids potential interference from hydrogen peroxide introduced by peroxidase-like nanomaterials, significantly enhancing sensitivity. reliability accuracy were validated, demonstrating determination acarbose an IC50 value 7.04 μM limit 3.2 nM. Moreover, has been successfully applied screen natural flavonoid compounds.
Language: Английский