Journal of Chromatography A, Journal Year: 2024, Volume and Issue: 1736, P. 465406 - 465406
Published: Sept. 30, 2024
Language: Английский
Journal of Food Science, Journal Year: 2025, Volume and Issue: 90(1)
Published: Jan. 1, 2025
Abstract This study aimed to investigate the potential hypoglycemic mechanism of red ginseng acidic polysaccharides (RGAP) from perspective fatty acid (FA) regulation. A high‐glucose/high‐fat diet in conjunction with streptozotocin administration was employed establish type 2 diabetes mellitus (T2DM) rat models, and their fecal FAs were detected using liquid chromatography‐mass spectrometry (LC‐MS) method. RGAP treatment alleviated polyphagia, polydipsia, weight loss, hyperglycemia observed T2DM rats. FA profile disturbed by modeling, 11 marker selected statistical analysis, whose intensities reversely changed administration. Among these FAs, short‐chain negatively correlated fasting blood glucose (FBG) level, while positive correlations between long‐chain FBG level. Combined metabolite‐enzyme‐gene network we inferred that mechanistic on may be associated regulation metabolism inflammation‐related signaling pathways. confirmed regulatory effect FA, which can provide a scientific basis new ideas for developing as functional food supplementary T2DM.
Language: Английский
Citations
0
Mass Spectrometry Reviews, Journal Year: 2025, Volume and Issue: unknown
Published: March 12, 2025
ABSTRACT Liquid chromatography‐mass spectrometry (LC‐MS) has become an indispensable tool for elucidating molecular structures and quantifying diverse compounds within complex mixtures. Despite its versatility, it faces various challenges such as ion suppression, low sensitivity, analyte instability, matrix effects, which are being overcome by different kinds of offline online derivatization techniques to improve specificity reduce potential interferences. In this context, considerable advancements have been made in reviewing critically evaluating a wide range developed methods techniques; however, little attention given post‐column (PCD) LC‐MS. Therefore, comprehensive review highlights state‐of‐the‐art LC‐MS with specific focus on types chemical physical PCD, in‐source derivatization. It also examines the latest instrumentation developments, influencing factors, explores applications food, proteomics, biology, pharmaceuticals, environmental analysis from past four decades. Besides, role PCD along outlining advantages disadvantages. Furthermore, special emphasis is prospects insights developing more versatile LC‐PCD‐MS methodologies, address ongoing aim open new research avenues analysts.
Language: Английский
Citations
0
Journal of Chromatography Open, Journal Year: 2025, Volume and Issue: unknown, P. 100215 - 100215
Published: March 1, 2025
Language: Английский
Citations
0
Analytica Chimica Acta, Journal Year: 2025, Volume and Issue: unknown, P. 344001 - 344001
Published: April 1, 2025
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 12410 - 12410
Published: Nov. 19, 2024
In neuroscience research, chiral metabolomics is an emerging field, in which D-amino acids play important role as potential biomarkers for neurological diseases. The targeted analysis of the brain metabolome, employing liquid chromatography (LC) coupled to mass spectrometry (MS), a pivotal approach identification This review provides overview diseases and state-of-the-art strategies enantioselective amino (AAs) biological samples investigate their putative Fluctuations (D-AAs) levels can be related pathology diseases, example, through modulation N-methyl-D-aspartate receptors neurotransmission. Because trace presence these biomolecules mammals complex nature matrices, highly sensitive selective analytical methods are essential. Derivatization with reagents highlighted critical tools enhancing detection capabilities. latest advances derivatization reactions, LC-MS/MS analysis, have improved quantification AAs allow separation several metabolites single run. enhanced performances provide accurate correlation between specific D-AA profiles disease states, allowing better understanding drug effects on brain.
Language: Английский
Citations
3
Biomedicines, Journal Year: 2024, Volume and Issue: 12(5), P. 1003 - 1003
Published: May 2, 2024
Many biologically active metabolites of the essential amino acid L-tryptophan (Trp) are associated with different neurodegenerative diseases and neurological disorders. Precise reliable methods for their determination needed. Variability in physicochemical properties makes analytical process challenging. In this case, chemical modification analyte derivatization could come into play. Here, we introduce a novel fast reversed-phase ultra-high-performance liquid chromatography (RP-UHPLC) coupled tandem mass spectrometry (MS/MS) method Trp its ten human plasma samples after 2-bromo-4′-nitroacetophenone (BNAP). The procedure was optimized terms incubation time, temperature, concentration, volume reagent. Method development comprises choice suitable stationary phase, mobile phase composition, gradient elution optimization. developed validated according to ICH guidelines. Results all validation parameters were within acceptance criteria guideline, i.e., intra- inter-day precision (expressed as relative standard deviation; RSD) range 0.5–8.2% 2.3–7.4%, accuracy 93.3–109.7% 94.7–110.1%, limits detection (LODs) 0.15–9.43 ng/mL, coefficients (R2) higher than 0.9906, carryovers were, cases, less 8.8%. practicability evaluated using blue applicability grade index (BAGI) score 65. Finally, used analysis Alzheimer’s disease healthy control prove applicability. Statistical revealed significant changes picolinic (PA), anthranilic (AA), 5 hydroxyindole-3-acetic (5-OH IAA), quinolinic (QA) concentration levels. This serve basis future studies that will be conducted large cohort patients.
Language: Английский
Citations
1
Journal of Chromatography A, Journal Year: 2024, Volume and Issue: 1736, P. 465406 - 465406
Published: Sept. 30, 2024
Language: Английский
Citations
0