Microbial diversity of high-elevated fumarole fields - low-biomass communities on the boundary between ice and fire
Abstract
Fumarole
fields
on
active
volcanoes
are
habitats
that
host
unique
microbial
ecosystems.
However,
DNA
extraction
from
them
for
further
analysis
is
rather
challenging.
In
this
study,
we
compared
two
different
ways
of
sample
homogenization
to
profile
the
communities
fumarolic
Elbrus
and
Ushkovsky
frozen
fumarole
deposits
Fujiyama.
Vertical
homogeniser
gave
significantly
higher
concentrations
samples,
more
archaeal
amplicon
sequence
variants
(ASVs)
samples
horizontal
one.
This
suggests
vertical
might
be
preferable
sandy
rocky
soils.
Independent
homogenizer
type,
dominant
phyla
were
Acidobacteriota
Pseudomonadota ,
Crenarchaeota
Ushkovsky.
The
bacterial
community
Fuji
was
less
diverse,
with
Actinomycetota ,
Pseudomonadota
Bacillota
being
phyla.
Thus,
studied
fumaroles
showed
distinct
profiles,
revealing
adaptations
their
respective
extreme
environments.
Within
fungal
community,
Ascomycota ,
Basidiomycota
Chytridiomycota
most
all
three
volcanoes,
but
abundance
varied.
study
offers
first
comprehensive
fields,
demonstrates
choice
methodology
can
influence
understanding
diversity
in
Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 26, 2024
Language: Английский