Recent implementations of data-independent acquisition for cancer biomarker discovery in biological fluids DOI Creative Commons
Annie Ha, Michael Woolman, Matthew Waas

et al.

Expert Review of Proteomics, Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

Cancer is the second leading cause of death worldwide and accurate biomarkers for early detection disease monitoring are needed to improve outcomes. Biological fluids, such as blood urine, ideal samples biomarker measurements they can be routinely collected with relatively minimally invasive methods. However, proteomics analysis fluids has been a challenge due high dynamic range its protein content. Advances in data-independent acquisition (DIA) mass spectrometry-based address some technical challenges biofluids, thus enabling ability spectrometry propel large-scale discovery. We reviewed principles DIA recent applications cancer discovery using biofluids. summarized studies biological context research over past decade, provided comprehensive overview benefits DIA-MS. Various showed potential DIA-MS identifying putative high-throughput manner. lack proper study design standardization methods across platforms still need addressed fully utilize accelerate verification processes.

Language: Английский

Recent implementations of data-independent acquisition for cancer biomarker discovery in biological fluids DOI Creative Commons
Annie Ha, Michael Woolman, Matthew Waas

et al.

Expert Review of Proteomics, Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

Cancer is the second leading cause of death worldwide and accurate biomarkers for early detection disease monitoring are needed to improve outcomes. Biological fluids, such as blood urine, ideal samples biomarker measurements they can be routinely collected with relatively minimally invasive methods. However, proteomics analysis fluids has been a challenge due high dynamic range its protein content. Advances in data-independent acquisition (DIA) mass spectrometry-based address some technical challenges biofluids, thus enabling ability spectrometry propel large-scale discovery. We reviewed principles DIA recent applications cancer discovery using biofluids. summarized studies biological context research over past decade, provided comprehensive overview benefits DIA-MS. Various showed potential DIA-MS identifying putative high-throughput manner. lack proper study design standardization methods across platforms still need addressed fully utilize accelerate verification processes.

Language: Английский

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