Nanocarrier-mediated RNAi of CYP9E2 and CYB5R enhance susceptibility of invasive tomato pest, Tuta absoluta to cyantraniliprole DOI Creative Commons
Farman Ullah, Guru‐Pirasanna‐Pandi Govindharaj, Hina Gul

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: April 28, 2025

Cyantraniliprole, a second-generation anthranilic diamide, is widely used to manage lepidopteran pests, including the invasive tomato pinworm Tuta absoluta (Meyrick). However, cyantraniliprole's resistance mechanisms and associated fitness costs in T. remain underexplored. Here, we investigated of cyantraniliprole-resistant strain (CyanRS) via nanocarrier-mediated RNA interference (RNAi). Results showed that egg incubation period developmental durations larval pupal stages were significantly prolonged CyanRS population compared susceptible (SS). Further, adult emergence, longevities male female, fecundity, hatching rate reduced individuals. The mRNA expression levels cytochrome b5 reductase (CYB5R) P450 (CYP9E2) analyzed using RT-qPCR explore their potential involvement cyantraniliprole absoluta. Phylogenetic motif analysis CYB5R CYP9E2 indicated evolutionary functional conservation with other insect species, especially Lepidopterans. Notably, nanocarrier-encapsulated dsRNA targeting genes levels. activity was substantially decreased after knockdown CYP92 genes. This increased susceptibility resistant cyantraniliprole, leading higher mortality than controls. These findings show might play key role evolution current study provides in-depth insights understand underlying this herbivore.

Language: Английский

Nanocarrier-mediated RNAi of CYP9E2 and CYB5R enhance susceptibility of invasive tomato pest, Tuta absoluta to cyantraniliprole DOI Creative Commons
Farman Ullah, Guru‐Pirasanna‐Pandi Govindharaj, Hina Gul

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: April 28, 2025

Cyantraniliprole, a second-generation anthranilic diamide, is widely used to manage lepidopteran pests, including the invasive tomato pinworm Tuta absoluta (Meyrick). However, cyantraniliprole's resistance mechanisms and associated fitness costs in T. remain underexplored. Here, we investigated of cyantraniliprole-resistant strain (CyanRS) via nanocarrier-mediated RNA interference (RNAi). Results showed that egg incubation period developmental durations larval pupal stages were significantly prolonged CyanRS population compared susceptible (SS). Further, adult emergence, longevities male female, fecundity, hatching rate reduced individuals. The mRNA expression levels cytochrome b5 reductase (CYB5R) P450 (CYP9E2) analyzed using RT-qPCR explore their potential involvement cyantraniliprole absoluta. Phylogenetic motif analysis CYB5R CYP9E2 indicated evolutionary functional conservation with other insect species, especially Lepidopterans. Notably, nanocarrier-encapsulated dsRNA targeting genes levels. activity was substantially decreased after knockdown CYP92 genes. This increased susceptibility resistant cyantraniliprole, leading higher mortality than controls. These findings show might play key role evolution current study provides in-depth insights understand underlying this herbivore.

Language: Английский

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