Investigative Ophthalmology & Visual Science,
Journal Year:
2024,
Volume and Issue:
65(14), P. 32 - 32
Published: Dec. 18, 2024
Intraocular
pressure
(IOP)
elevation
is
the
primary
risk
factor
and
currently
main
treatable
for
progression
of
glaucomatous
optic
neuropathy.
In
addition
to
direct
clinical
living
animal
in
vivo
studies,
ex
perfusion
anterior
segments
whole
eyes
a
key
technique
studying
conventional
outflow
function
as
it
responsible
IOP
regulation.
We
present
well-tested
experimental
details,
protocols,
considerations,
advantages,
limitations
several
model
systems
These
include:
(1)
perfused
globes,
(2)
stationary
segment
organ
culture,
(3)
human
(4)
(5)
corneal
rims,
(6)
wedges.
methods,
with
due
consideration
paid
their
strengths
limitations,
comprise
set
very
strong
tools
extending
our
understanding
Biomedical Optics Express,
Journal Year:
2024,
Volume and Issue:
15(2), P. 1059 - 1059
Published: Jan. 18, 2024
A
real-time
line-field
optical
coherence
tomography
(LF-OCT)
system
is
demonstrated
with
image
acquisition
rates
of
up
to
5000
B-frames
or
2.5
million
A-lines
per
second
for
500
B-frame.
The
uses
a
high-speed
low-cost
camera
achieve
continuous
data
transfer
required
imaging,
allowing
the
evaluation
future
applications
in
clinical
intraoperative
environments.
light
source
an
840
nm
super-luminescent
diode.
Leveraging
parallel
computing
GPU
and
high
speed
CoaXPress
interface,
we
were
able
acquire,
process,
display
OCT
low
latency.
studied
anamorphic
beam
shaping
detector
arm,
optimizing
field
view
sensitivity
imaging
biological
tissue
at
cellular
resolution.
lateral
axial
resolution
measured
air
1.7
µm
6.3
µm,
respectively.
Experimental
results
demonstrate
inspection
trabecular
meshwork
Schlemm’s
canal
on
ex
vivo
corneoscleral
wedges
endothelial
cells
human
subjects
.
Bioengineering,
Journal Year:
2023,
Volume and Issue:
10(9), P. 1038 - 1038
Published: Sept. 4, 2023
Background:
More
than
~70%
of
the
aqueous
humor
exits
eye
through
conventional
outflow
pathway
that
is
comprised
trabecular
meshwork
(TM),
juxtacanalicular
tissue
(JCT),
inner
wall
endothelium
Schlemm’s
canal
(SC).
The
flow
resistance
in
JCT
and
SC
basement
membrane
thought
to
play
an
important
role
regulation
intraocular
pressure
(IOP)
eye,
but
current
imaging
techniques
do
not
provide
enough
information
about
mechanics
these
tissues
or
this
area.
Methods:
A
normal
human
was
perfusion-fixed
a
radial
wedge
TM
from
high-flow
region
dissected.
were
then
sliced
imaged
using
serial
block-face
scanning
electron
microscopy.
Slices
images
selected
segmented
create
3D
finite
element
model
cells
with
membrane.
used
replace
intertrabecular
spaces,
pores,
giant
vacuoles,
fluid–structure
interaction
employed
couple
motion
humor.
Results:
Higher
tensile
stresses
(0.8-kPa)
strains
(25%)
observed
beneath
vacuoles
open
pores.
volumetric
average
shear
stress
higher
JCT/SC.
As
approached
SC,
velocity
decreased,
resulting
formation
small
eddies
immediately
after
left
wall.
Conclusions:
Improved
modeling
can
enhance
our
understanding
funneling.
Serial
microscopy
achieve
this,
micro-segmental
patterns
ex
vivo
perfused
eyes
suggest
hypothetical
mechanism.
