Injectable microspheres filled with copper-containing bioactive glass improve articular cartilage healing by regulating inflammation and recruiting stem cells DOI Creative Commons
Hua Gao,

Eryu Ning,

Xiaoyu Zhang

et al.

Regenerative Biomaterials, Journal Year: 2024, Volume and Issue: 12

Published: Dec. 17, 2024

Abstract Osteoarthritis (OA) is a frequent chronic illness in orthopedics that poses major hazard to patient health. In situ cell therapy emerging as therapeutic option, but its efficacy influenced by both the inflammatory milieu and amount of stem cells, limiting use. this study, we designed novel injectable porous microsphere (PM) based on microfluidic technology can support mesenchymal cells (MSCs) combining polylactic–glycolic acid copolymer, kartogenin, polydopamine, stromal cell-derived factor-1, copper-doped bioactive glass (CuBG). The ex vivo tests demonstrated PMs@CuBG microspheres were biocompatible facilitated transformation synovial macrophages from pro-inflammatory M1 anti-inflammatory M2 phenotypes releasing CuBG reduce joint inflammation. At same time, are able recruit MSCs into cavity encourage their differentiation chondrocytes, thereby treating articular cartilage injury. rat experimental results show intra-articular injection rats with OA improves OARSI scores, aggrecan content ratio col-2α-positive indicating reparative effect damaged within joint. As result, predicted provide successful approach for OA.

Language: Английский

An in situ forming cartilage matrix mimetic hydrogel scavenges ROS and ameliorates osteoarthritis after superficial cartilage injury DOI Creative Commons
Zhicheng Tong,

Yuanzhu Ma,

Qiushi Liang

et al.

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 187, P. 82 - 97

Published: Aug. 23, 2024

Superficial cartilage defects represent the most prevalent type of injury encountered in clinical settings, posing significant treatment challenges. Here, we fabricated a extracellular matrix mimic hydrogel (GHC, consisting Gelatin, Hyaluronic acid, and Chondroitin sulfate) to avoid exacerbation deterioration, which is often driven by accumulation reactive oxygen species (ROS) pro-inflammatory microenvironment. The GHC exhibited multifunctional properties, including situ formation, tissue adhesiveness, anti-ROS capabilities, promotion chondrogenesis. enhancement adhesion was achieved chemically modifying hyaluronic acid chondroitin sulfate with o-nitrobenzene, enabling covalent connection surface upon light irradiation. In vitro characterization revealed that facilitated chondrocyte adhesion, migration, differentiation into cartilage. Additionally, hydrogels demonstrated ability scavenge ROS inhibit production inflammatory factors chondrocytes. animal model superficial injury, effectively promoted ECM regeneration interface integration between host material. These findings suggest hold considerable promise as strategy for defect repair. STATEMENT OF SIGNIFICANCE: clinic. Previous engineering materials are only suitable full-thickness or osteochondral defects. developed composed gelatin, sulfate, natural components. drug-free cell-free not avoids immune rejection drug toxicity, but also shows good mechanical properties biocompatibility. More importantly, could adhere tightly promote while protecting against oxidation. This ingredients potential material repairing

Language: Английский

Citations

6
Polydopamine-based nano-protectant for prolonged boar semen preservation by eliminating ROS and regulating protein phosphorylation via D2DR-mediated cAMP/PKA signaling pathway DOI Creative Commons
Lirui Wang,

Manyi Xiong,

Jian Zhang

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 27, 2025

Language: Английский

Citations

0
Multifunctional piezoelectric hydrogels under ultrasound stimulation boost chondrogenesis by recruiting autologous stem cells and activating the Ca2+/CaM/CaN signaling pathway DOI
Yubao Liu, Xu Liu, Xiaofei Li

et al.

Bioactive Materials, Journal Year: 2025, Volume and Issue: 50, P. 344 - 363

Published: April 18, 2025

Language: Английский

Citations

0
Recent Advances in Multifunctional Naturally Derived Bioadhesives for Tissue Engineering and Wound Management DOI
Aliakbar Jafari, Ahmed Al‐Ostaz, Sasan Nouranian

et al.

Polymers for Advanced Technologies, Journal Year: 2024, Volume and Issue: 35(12)

Published: Dec. 1, 2024

ABSTRACT Recent advancements in naturally derived bioadhesives have transformed their application across diverse medical fields, including tissue engineering, wound management, and surgery. This review focuses on the innovative development multifunctional nature of these bioadhesives, particularly emphasizing role enhancing adhesion performance wet environments optimizing mechanical properties for use dynamic tissues. Key areas covered include chemical physical mechanisms adhesion, incorporation multi‐adhesion strategies that combine covalent non‐covalent bonding, bioinspired designs mimicking natural adhesives such as those barnacles mussels. Additionally, discusses emerging applications regeneration musculoskeletal, cardiac, neural, ocular tissues, highlighting potential bioadhesive‐based therapies complex biological settings. Despite substantial progress, challenges scaling lab‐based innovations clinical overcoming environmental constraints remain critical. Ongoing research bioadhesive technologies aims to bridge gaps, promising significant improvements tailored therapeutic needs.

Language: Английский

Citations

3
E7 peptide for in situ tissue engineering applications in bone repair DOI
Wentao Zhang, Kaizhong Wang, Moran Suo

et al.

Journal of the American Ceramic Society, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Abstract In regenerative medicine, leveraging bioactive molecules to enhance endogenous repair mechanisms represents a critical advancement. The E7 peptide, novel short has emerged as key candidate for bone defect due its unique ability interact with stem cells directly. Unlike traditional tissue‐engineered constructs that rely on exogenous cell delivery or scaffold‐based strategies, enables in situ regeneration by actively recruiting and anchoring the site. Studies demonstrate E7‐functionalized materials significantly proliferation osteogenic differentiation while concurrently stimulating local angiogenesis through VEGF upregulation. These dual effects—stem homing vascularization—address two major bottlenecks repair: insufficient supply poor nutrient diffusion avascular regions. Despite these advantages, optimizing E7's spatiotemporal presentation elucidating signaling remain critical. Further investigations into receptor specificity, dose dependency, long‐term safety will accelerate clinical translation. It is of great guiding significance clarify what role. peptide plays various which pathways are activated future research repair.

Language: Английский

Citations

0
Effect and potential mechanism of modified citrus pectin in 3D printing-based cartilage tissue engineering DOI
Danning Su, Jiayue He, Wenlong Yuan

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 311, P. 144022 - 144022

Published: May 8, 2025

Language: Английский

Citations

0
Strontium-doped bioactive glass-functionalized polyetheretherketone enhances osseointegration by facilitating cell adhesion DOI Creative Commons
Zihao Xu,

Licai Miao,

Xiangyu Meng

et al.

Colloids and Surfaces B Biointerfaces, Journal Year: 2024, Volume and Issue: 241, P. 114042 - 114042

Published: June 15, 2024

In the field of orthopedics, surgeons have long been facing challenge loosening external fixation screws due to inherent material characteristics. Despite Polyetheretherketone (PEEK) being employed as an orthopedic implant for many years, its bio-inert nature often hinders bone healing limited bioactivity, which restricts clinical applications. Herein, a new type (Sr-SPK) was developed by introducing strontium (Sr)-doped mesoporous bioactive glass (Sr-MBG) onto surface PEEK implants through simple and feasible method. vitro experiments revealed that Sr-SPK effectively promotes osteogenic differentiation while concurrently suppressing formation osteoclasts. The same results were validated in vivo with significantly improving integration. Upon investigation, it found adhesion among marrow mesenchymal stem cells (BMSCs) thereby promoting osteogenesis activating regulation actin cytoskeletal focal pathways, identified via transcriptome analysis. essence, these findings suggest newly constructed Sr-doped biofunctionalized this research can promote osteoblast suppress osteoclast activity enhancing cell processes. These underline immense potential such wide-ranging applications orthopedics.

Language: Английский

Citations

2
Injectable microspheres filled with copper-containing bioactive glass improve articular cartilage healing by regulating inflammation and recruiting stem cells DOI Creative Commons
Hua Gao,

Eryu Ning,

Xiaoyu Zhang

et al.

Regenerative Biomaterials, Journal Year: 2024, Volume and Issue: 12

Published: Dec. 17, 2024

Abstract Osteoarthritis (OA) is a frequent chronic illness in orthopedics that poses major hazard to patient health. In situ cell therapy emerging as therapeutic option, but its efficacy influenced by both the inflammatory milieu and amount of stem cells, limiting use. this study, we designed novel injectable porous microsphere (PM) based on microfluidic technology can support mesenchymal cells (MSCs) combining polylactic–glycolic acid copolymer, kartogenin, polydopamine, stromal cell-derived factor-1, copper-doped bioactive glass (CuBG). The ex vivo tests demonstrated PMs@CuBG microspheres were biocompatible facilitated transformation synovial macrophages from pro-inflammatory M1 anti-inflammatory M2 phenotypes releasing CuBG reduce joint inflammation. At same time, are able recruit MSCs into cavity encourage their differentiation chondrocytes, thereby treating articular cartilage injury. rat experimental results show intra-articular injection rats with OA improves OARSI scores, aggrecan content ratio col-2α-positive indicating reparative effect damaged within joint. As result, predicted provide successful approach for OA.

Language: Английский

Citations

0