Effect of fermentation on the constituents in the branches and leaves of Taxus media and non-small cell lung cancer DOI Creative Commons

Xing Guo,

Rui‐Sheng Wang, Zhenling Zhang

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 23, 2024

Introduction Non-small cell lung cancer (NSCLC) is a prominent disease worldwide. Currently, commonly used methods, such as surgery and radiotherapy, have significant side effects. Traditional Chinese medicine (TCM) has become research hotspot because of its safe effective characteristics. The branches leaves Taxus media are abundant in antitumor active compounds, there been no conducted yet regarding anti–lung molecular mechanism. Objective aim this study to investigate the activity two samples before after fermentation T. , mechanism inhibitory effect on NSCLC. Methods chemical composition pre-fermentation (TM) post-fermentation qu (TMQ) were investigated using UHPLC-Q-Qrbitrap HRMS high-performance liquid chromatography (HPLC). anti-lung activities TM TMQ compared an A549-induced tumor mouse model. An enzyme-linked immunosorbent assay (ELISA), hematoxylin eosin (H&E) staining, immunohistochemistry, immunofluorescence determine action. Results results indicated that contained 83 consisting primarily flavonoids, organic acids, taxanes. Both taxanes flavonoids higher than TM. effectively inhibited growth non-small (NSCLC), inhibition rate was greater (57.24%) (49.62%). administration downregulated necrosis factor-α (TNF-α), interleukin-6 (IL-6), glutathione (GSH) level upregulated interferon-γ (IFN-γ), reactive oxygen species (ROS), malondialdehyde (MDA) levels serum mice. treatment also increased protein expression Bax, Caspase-3, Beclin-1 tissues. In contrast, bcl-2, PI3K, Ki67, ULK1, mTOR suppressed by TMQ. Protein analyses reemphasized superior over These cumulative findings demonstrated action closely related activation transcriptional misregulation pathway cholinergic synaptic, AMPK, PI3K/Akt/mTOR signaling pathways. Conclusion This ingredient contents effects . addition, herbal for NSCLC treatment.

Language: Английский

A Peptide‐Derived Aggregation‐Induced Emission Nanobioprobe: Unlocking Selective Detection of Fusobacterium nucleatum and Noninvasive Screening of Colorectal Cancer DOI Creative Commons
Hongyu Liu,

T.-C. Wu,

Yunjian Yu

et al.

Aggregate, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

ABSTRACT Colorectal cancer (CRC) screening and early diagnosis is an effective strategy for reducing CRC mortality. However, the current detection methods involve exorbitant costs complex procedures, which are inconvenient large‐scale screening. Given its high prevalence in malignant tissues feces of patients, Fusobacterium nucleatum ( F. ) has emerged as a crucial biomarker CRC. Herein, we propose ‐specific recognition diagnosis. A novel nanobioprobe (AIE‐Pep) with aggregation‐induced emission (AIE) characteristics was synthesized by conjugating red/near‐infrared (NIR) emissive AIE luminogen (AIEgen) FadA‐targeting peptide (ASANWTIQYND). The robust binding affinity between FadA on allows AIE‐Pep NPs to adhere selectively , emits strong red/NIR fluorescence. In model orthotopic CRC, can precisely localize around Moreover, demonstrated limit (LOD) 82.97 CFU/mL could significantly differentiate mice from those normal mice. Overall, this study presents pivotal approach specifically identifying holds immense potential

Language: Английский

Citations

1
Neutrophil extracellular traps in tumor metabolism and microenvironment DOI Creative Commons

Z. Liu,

Yuanyao Dou, Conghua Lu

et al.

Biomarker Research, Journal Year: 2025, Volume and Issue: 13(1)

Published: Jan. 23, 2025

Neutrophil extracellular traps (NETs) are intricate, web-like formations composed of DNA, histones, and antimicrobial proteins, released by neutrophils. These structures participate in a wide array physiological pathological activities, including immune rheumatic diseases damage to target organs. Recently, the connection between NETs cancer has garnered significant attention. Within tumor microenvironment metabolism, exhibit multifaceted roles, such as promoting proliferation migration cells, influencing redox balance, triggering angiogenesis, driving metabolic reprogramming. This review offers comprehensive analysis link emphasizing areas that remain underexplored. include interaction with mitochondria, their effect on states within tumors, involvement reprogramming, contribution angiogenesis tumors. Such insights lay theoretical foundation for deeper understanding role development. Moreover, also delves into potential therapeutic strategies suggests future research directions, offering new perspectives treatment other related diseases.

Language: Английский

Citations

1
Unraveling the Role of Fusobacterium nucleatum in Colorectal Cancer: Molecular Mechanisms and Pathogenic Insights DOI Open Access

Linda Galasso,

Fabrizio Termite, Irene Mignini

et al.

Cancers, Journal Year: 2025, Volume and Issue: 17(3), P. 368 - 368

Published: Jan. 23, 2025

Fusobacterium nucleatum, a gram-negative anaerobic bacterium, has emerged as significant player in colorectal cancer (CRC) pathogenesis. The bacterium causes persistent inflammatory reaction the mucosa by stimulating release of pro-inflammatory cytokines like IL-1β, IL-6, and TNF-α, creating an environment conducive to progression. F. nucleatum binds penetrates epithelial cells through adhesins such FadA, impairing cell junctions encouraging epithelial-to-mesenchymal transition (EMT), which is associated with advancement. Additionally, modulates host immune system, suppressing activity conditions favorable for tumor growth. Its interactions gut microbiome contribute dysbiosis, further influencing carcinogenic pathways. Evidence indicates that can inflict DNA damage either directly via reactive oxygen species or indirectly environment. it triggers oncogenic pathways, especially Wnt/β-catenin signaling pathway, promotes growth longevity. Moreover, alters microenvironment, impacting behavior, metastasis, therapeutic responses. purpose this review elucidate molecular mechanisms contributes CRC. Understanding these crucial development targeted therapies diagnostic strategies CRC nucleatum.

Language: Английский

Citations

1
Tumor and intratumoral pathogen cascade-targeting photothermal nanotherapeutics for boosted immunotherapy of colorectal cancer DOI

Youtao Xin,

Yunjian Yu,

Mengdi Wu

et al.

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: 379, P. 574 - 591

Published: Jan. 24, 2025

Language: Английский

Citations

0
Oral microbiota in colorectal cancer: Unraveling mechanisms and application potential DOI
Xinran Zhang, Yixin Chen, Yuwei Xia

et al.

Life Sciences, Journal Year: 2025, Volume and Issue: 365, P. 123462 - 123462

Published: Feb. 11, 2025

Language: Английский

Citations

0
Dual delivery of agmatine and microRNA-126b using agmatine-mediated DNA nanotube assemblies for acute lung injury therapy DOI
Chunfa Chen, Quan Li,

Beinuo Wang

et al.

Acta Biomaterialia, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Effect of fermentation on the constituents in the branches and leaves of Taxus media and non-small cell lung cancer DOI Creative Commons

Xing Guo,

Rui‐Sheng Wang, Zhenling Zhang

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 23, 2024

Introduction Non-small cell lung cancer (NSCLC) is a prominent disease worldwide. Currently, commonly used methods, such as surgery and radiotherapy, have significant side effects. Traditional Chinese medicine (TCM) has become research hotspot because of its safe effective characteristics. The branches leaves Taxus media are abundant in antitumor active compounds, there been no conducted yet regarding anti–lung molecular mechanism. Objective aim this study to investigate the activity two samples before after fermentation T. , mechanism inhibitory effect on NSCLC. Methods chemical composition pre-fermentation (TM) post-fermentation qu (TMQ) were investigated using UHPLC-Q-Qrbitrap HRMS high-performance liquid chromatography (HPLC). anti-lung activities TM TMQ compared an A549-induced tumor mouse model. An enzyme-linked immunosorbent assay (ELISA), hematoxylin eosin (H&E) staining, immunohistochemistry, immunofluorescence determine action. Results results indicated that contained 83 consisting primarily flavonoids, organic acids, taxanes. Both taxanes flavonoids higher than TM. effectively inhibited growth non-small (NSCLC), inhibition rate was greater (57.24%) (49.62%). administration downregulated necrosis factor-α (TNF-α), interleukin-6 (IL-6), glutathione (GSH) level upregulated interferon-γ (IFN-γ), reactive oxygen species (ROS), malondialdehyde (MDA) levels serum mice. treatment also increased protein expression Bax, Caspase-3, Beclin-1 tissues. In contrast, bcl-2, PI3K, Ki67, ULK1, mTOR suppressed by TMQ. Protein analyses reemphasized superior over These cumulative findings demonstrated action closely related activation transcriptional misregulation pathway cholinergic synaptic, AMPK, PI3K/Akt/mTOR signaling pathways. Conclusion This ingredient contents effects . addition, herbal for NSCLC treatment.

Language: Английский

Citations

0