Injectable acellular matrix microgel assembly with stem cell recruitment and chondrogenic differentiation functions promotes microfracture-based articular cartilage regeneration

Bioactive Materials, Journal Year: 2024, Volume and Issue: 44, P. 220 - 235

Published: Oct. 19, 2024

Language: Английский

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Emerging Technological Advancement for Chronic Wound Treatment and Their Role in Accelerating Wound Healing

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: 7(11), P. 7101 - 7132

Published: Oct. 28, 2024

Chronic wounds are a major healthcare burden and may severely affect the social, mental, economic status of patients. Any impairment in wound healing stages due to underlying factors leads prolonged time subsequently chronic wounds. Traditional approaches for treatment include dressing free local therapy, tissue engineering based scaffold therapies. However, traditional therapies need improvisation have been advanced through breakthrough technologies. The present review spans further gives an extensive account advancements Cutting edge technologies, such as 3D printing, which includes inkjet fused deposition modeling, digital light processing, extrusion-based microneedle array-based therapies, gene microRNAs (miRNAs) smart dressings real monitoring conditions assessment pH, temperature, oxygen, moisture, metabolites, their use planning better strategies discussed detail. future direction treatments that will aid lowering caused

Language: Английский

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Enhanced Cell Proliferation, Migration, and Fibroblast Differentiation with Electrospun PCL–Zinc Scaffolds Coated with Fibroblast-Derived ECM

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Despite tremendous improvement in the development of tissue-regenerating materials, a promising solution that provides an optimal environment remains to be accomplished. Here, we report composite nanofiber biomaterial scaffold as closely mimics extracellular matrix (ECM) improve cell viability, proliferation, and migration. Initially, composites polycaprolactone (PCL) zinc (Zn) metal were fabricated by using electrospinning. The resulting PCL–Zn (PZ) nanofibers effectively guided growth NIH3T3 fibroblasts for 7 days, forming fibroblast sheet. PZ fibers decellularized remove autologous allogenic cellular antigens while leaving intact ECM with structural functional components. PCL–Zn–ECM (PZE) showcased natural bonded surface, providing bioactive element interconnected fibers. reseeding demonstrated scaffold's excellent capacity direct support proliferation. Furthermore, vitro cytotoxicity analysis morphological staining confer biocompatibility. PZE presents which these scaffolds can further used various regenerative medicine applications including wound healing.

Language: Английский

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Spontaneous bone regeneration achieved through one-step alignment of human mesenchymal stem cell-embedded collagen

Acta Biomaterialia, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Optimizing cell-matrix interactions for effective bone regeneration remains a significant hurdle in tissue engineering. This study presents novel approach by developing hMSC-embedded 3D aligned collagen enhanced regeneration. A one-step mechanical strain was applied to mixture of hMSCs and collagen, producing an hMSC-embedded, hydrogel patch that mimics the natural matrix. Notably, embedded spontaneously differentiated into osteoblasts without external inducing reagents. Immunofluorescence analysis revealed BMP2-smad1/5 signaling pathway, critical osteogenic differentiation, were activated collagen. In vivo experiments using calvarial defect model confirmed this effectively promotes new formation, starting centrally within rather than from edges adjacent existing bone. Our findings suggest simple method pre-straining create with holds promise as cell therapy platform STATEMENT OF SIGNIFICANCE: : introduces enhancing human mesenchymal stem cells (hMSCs). single hMSC-collagen produces matrix tissue. Remarkably, differentiate absence exogenous reagents triggered activation morphogenetic protein pathway. studies confirm regeneration, initiating generation center defect. offers promising repair, broad implications engineering regenerative medicine.

Language: Английский

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Bicomponent nano- and microfiber aerogels for effective management of junctional hemorrhage

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 11, 2025

Abstract Managing junctional hemorrhage is challenging due to ineffective existing techniques, with the groin being most common site, accounting for approximately 19.2% of potentially survivable field deaths. Here, we report a bicomponent nano- and microfiber aerogel (NMA) injection into deep, narrow wounds effectively halt bleeding. The comprises intertwined poly(lactic acid) nanofibers poly(ε-caprolactone) microfibers, mechanical properties tunable through crosslinking. Optimized aerogels demonstrate improved resilience, toughness, elasticity, enabling rapid re-expansion upon blood contact. They superior absorption clotting efficacy compared commercial products (i.e., QuikClot® Combat Gauze XStat®). Most importantly, in lethal swine wound model (Yorkshire swine, both male female, n = 5), treatment achieved immediate hemostasis, 100% survival rate, no rebleeding, hemodynamic stability, stable coagulation, hematologic, arterial gas testing.

Language: Английский

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MOF‐Enhanced Phototherapeutic Wound Dressings Against Drug‐Resistant Bacteria

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 26, 2024

Phototherapy is a low-risk alternative to traditional antibiotics against drug-resistant bacterial infections. However, optimizing phototherapy agents, refining treatment conditions, and addressing misuse of remain formidable challenge. This study introduces novel concept leveraging the unique customizability metal-organic frameworks (MOFs) house size-matched dye molecules in "single rooms". The mesoporous iron(III) carboxylate nanoMOF, MIL-100(Fe), hydrophobic heptamethine cyanine photothermal (Cy7), IR775, are selected as model systems. Their combination predicted minimize dye-dye interactions, leading exceptional photostability efficient light-to-heat conversion. Furthermore, MIL-100(Fe) preserves antimicrobial nature enabling it disrupt cell envelopes. Through electrospinning, MIL-100(Fe)@IR775 nanoparticles shaped into gelatin-based film dressing for skin wounds infected by Methicillin-resistant Staphylococcus aureus (MRSA). Activation requires only portable near-infrared light-emitting diode (NIR LED) induces both low-dose photodynamic therapy (LPDT) mild-temperature (MPTT). Combined with properties IR775 ferroptosis-like lipid peroxidation induced photoactive eradicates MRSA healing quick uninfected wounds. safe, cost-effective, multifunctional therapeutic wound offers promising solution overcome current bottleneck phototherapy.

Language: Английский

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Micro- and nano-fibers for organ-on-a-chip: Construction, applications, and prospects

Materials Today Bio, Journal Year: 2024, Volume and Issue: 29, P. 101322 - 101322

Published: Nov. 1, 2024

Language: Английский

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