Structural and biological investigation of alginate-nano-hydroxyapatite with chitosan-hyaluronic acid for potential osteochondral regeneration DOI
Seyed Abdolvahab Banihashemian, Soheila Zamanlui Benisi, Simzar Hosseinzadeh

et al.

International Journal of Polymeric Materials, Journal Year: 2023, Volume and Issue: 73(10), P. 851 - 865

Published: May 25, 2023

Cartilage joint lesions have created a significant challenge in the field of durable reconstruction and recovery due to their limited long-term repair reconstruction. In this study, biphasic scaffold was obtained using chitosan (CS)-hyaluronic acid (HA) alginate-nanohydroxyapatite (nHAP) for osteoarthritis diseases. The scaffolds revealed porous structures hydrophilic chemical groups by scanning electron microscopy (SEM) Fourier-transform infrared spectroscopy (FTIR) methods, respectively. resulting three-dimensional hydrogels were assembled fibrin glue seeded with human chondrocytes cells (hCHCs) chondral layer adipose-derived mesenchymal stem (hAdMSCs) subchondral one. Then, assessments including 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), SEM, compressive assay, weight loss, alizarin red staining, alkaline phosphatase (ALP) activity, alcian blue staining Real-Time PCR performed. associated cell viability confirmed that there no interventions between types SEM illustrated attachment spreading. layers investigated separately detect ability cellular commitment. Finally, Real-time carried out on bilayer related observations approved osteogenic/chondrogenic differentiation both types. Taken together, Alg-nHAP/CS-HA provided an appropriate environment cartilage bone regenerations could be recommended all aspects osteochondral tissue engineering.

Language: Английский

Structural and biological investigation of alginate-nano-hydroxyapatite with chitosan-hyaluronic acid for potential osteochondral regeneration DOI
Seyed Abdolvahab Banihashemian, Soheila Zamanlui Benisi, Simzar Hosseinzadeh

et al.

International Journal of Polymeric Materials, Journal Year: 2023, Volume and Issue: 73(10), P. 851 - 865

Published: May 25, 2023

Cartilage joint lesions have created a significant challenge in the field of durable reconstruction and recovery due to their limited long-term repair reconstruction. In this study, biphasic scaffold was obtained using chitosan (CS)-hyaluronic acid (HA) alginate-nanohydroxyapatite (nHAP) for osteoarthritis diseases. The scaffolds revealed porous structures hydrophilic chemical groups by scanning electron microscopy (SEM) Fourier-transform infrared spectroscopy (FTIR) methods, respectively. resulting three-dimensional hydrogels were assembled fibrin glue seeded with human chondrocytes cells (hCHCs) chondral layer adipose-derived mesenchymal stem (hAdMSCs) subchondral one. Then, assessments including 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), SEM, compressive assay, weight loss, alizarin red staining, alkaline phosphatase (ALP) activity, alcian blue staining Real-Time PCR performed. associated cell viability confirmed that there no interventions between types SEM illustrated attachment spreading. layers investigated separately detect ability cellular commitment. Finally, Real-time carried out on bilayer related observations approved osteogenic/chondrogenic differentiation both types. Taken together, Alg-nHAP/CS-HA provided an appropriate environment cartilage bone regenerations could be recommended all aspects osteochondral tissue engineering.

Language: Английский

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