Role of mucous cells in the defence system of the cnidarian Actinia equina (Linnaeus, 1758): The keystone of evolution DOI Open Access
Alessio Alesci, Sebastián Marino,

Anthea Miller

et al.

Acta Zoologica, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 8, 2024

Abstract Cnidarians are the first phylum of metazoans to exhibit a functional mucus layer. In evolution, mucous cells perform classical secretory function and act as sentinels for external insults, particularly in mammals. However, this topic has not been much analysed lower metazoans. This study aims investigate role Actinia equina (Anthozoa, Hexacorallia) (Linnaeus, 1758) by using confocal microscopy bioinformatics. The results show colocalization Toll‐like receptor 2 (TLR2) Mucin2 (MUC2) on these cells, suggesting crosstalk between defence response secretion cnidarians. Furthermore, presence TLR2 surface suggests their involvement host responses, occurs vertebrates. Moreover, through silico analysis, we confirmed orthologous genes tested antibodies, providing additional evidence support our from an evolutionary perspective. These data suggest that play defensive throughout helping us understand how have developed adapted across phylogenesis. information also provides new insights into strategies invertebrates clarifies history.

Language: Английский

HMGB1/RAGE axis in tumor development: unraveling its significance DOI Creative Commons
Anqi Fan,

Mengxiang Gao,

Xuhuan Tang

et al.

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14

Published: March 1, 2024

High mobility group protein 1 (HMGB1) plays a complex role in tumor biology. When released into the extracellular space, it binds to receptor for advanced glycation end products (RAGE) located on cell membrane, playing an important development by regulating number of biological processes and signal pathways. In this review, we outline multifaceted functions HMGB1/RAGE axis, which encompasses proliferation, apoptosis, autophagy, metastasis, angiogenesis. This axis is instrumental progression, promoting angiogenesis while inhibiting through pivotal signaling pathways, including MAPK, NF-κB, PI3K/AKT, ERK, STAT3. Notably, small molecules, such as miRNA-218, ethyl pyruvate (EP), glycyrrhizin exhibit ability inhibit restraining development. Therefore, deeper understanding mechanisms tumors great importance, inhibitors targeting warrants further exploration.

Language: Английский

Citations

8
Pregnancy-induced changes in the toll-like receptor pathway in the ovine duodenum DOI

Linna Gao,

Haiquan Ding,

Qianqian Shi

et al.

Veterinary Immunology and Immunopathology, Journal Year: 2025, Volume and Issue: 282, P. 110900 - 110900

Published: Feb. 18, 2025

Language: Английский

Citations

0
Novel insights into differences in the whey proteome of porcine and human colostrum and mature milk DOI
Huiwen Zhao, Xueyan Cao,

Zhizhong Quan

et al.

Food Chemistry, Journal Year: 2025, Volume and Issue: 478, P. 143584 - 143584

Published: March 1, 2025

Language: Английский

Citations

0
giEco-Friendly Synthesis and Multifunctional Evaluation of Silver Nanoparticles Using Boleophthalmus dussumieri Mucus: Antibacterial, Anticancer, and Predictive Modeling Applications DOI

Fahimeh Saberi,

Ahmad Gharzi,

Ashraf Jazayeri

et al.

Journal of Drug Delivery Science and Technology, Journal Year: 2025, Volume and Issue: unknown, P. 106843 - 106843

Published: March 1, 2025

Language: Английский

Citations

0
Toll-like Receptor Type 2 and 13 Gene Expression and Immune Cell Profiles in Diploid and Triploid Sterlets (Acipenser ruthenus): Insights into Immune Competence in Polyploid Fish DOI Open Access
Olga Jabłońska,

Sara Duda,

Szczepan Gajowniczek

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 3986 - 3986

Published: April 23, 2025

Toll-like receptors (TLRs) are key components of the innate immune system in fish, responsible for recognizing pathogen-associated molecular patterns derived from bacteria, viruses, and fungi. The sterlet (Acipenser ruthenus), an endangered sturgeon species valued its meat caviar, is a promising model studying effects polyploidy on gene regulation. This study examined expression receptor type 2 (TLR2) 13 (TLR13) heart, liver, gills, spleen, kidney diploid triploid healthy sterlets using real-time PCR. TLR2 TLR13 were expressed all tissues both diploids triploids. In diploids, was highest lowest liver (p < 0.05). Similarly, triploids, no significant tissue-specific variation TLR observed > Comparisons between revealed higher heart These findings supported by leukocyte analysis, which showed significantly lower percentage lymphocytes proportion neutrophils triploids compared to diploids. Additionally, thrombocytes elevated provides first report polyploid offering new insights into modulation associated with sturgeons.

Language: Английский

Citations

0
Molecular Characterization and Expression Analysis of Four Toll-like Receptors (TLR) Genes: TLR2, TLR5S, TLR14 and TLR22 in Mastacembelus armatus under Aeromonas veronii Infection DOI
Kaifeng Wang,

Dingxian Chen,

Siyu Lin

et al.

Developmental & Comparative Immunology, Journal Year: 2025, Volume and Issue: unknown, P. 105345 - 105345

Published: Feb. 1, 2025

Language: Английский

Citations

0
Comparative analysis of milk fat globule membrane proteins between porcine and human colostrum by a label‐free proteomics approach DOI
Huiwen Zhao, Zhenghan Zhang, Xianjun Liu

et al.

International Journal of Dairy Technology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 8, 2024

This study aimed to investigate the similarities and differences in milk fat globule membrane (MFGM) proteins from porcine colostrum (PC) human (HC) based on a label‐free proteomics approach. In total, 3901 4027 MFGM were identified PC HC, respectively, including 3452 common proteins. Similar biological functions pathways found two groups, whereas quantities types of involved different. compared between effectively expanding proteome its bioinformatic database providing new insights for exploration milk.

Language: Английский

Citations

2
Internal Defense System of Mytilus galloprovincialis (Lamarck, 1819): Ecological Role of Hemocytes as Biomarkers for Thiacloprid and Benzo[a]Pyrene Pollution DOI Creative Commons
Alessio Alesci,

Davide Di Paola,

Angelo Fumia

et al.

Toxics, Journal Year: 2023, Volume and Issue: 11(9), P. 731 - 731

Published: Aug. 25, 2023

The introduction of pollutants, such as thiacloprid and benzo[a]pyrene (B[a]P), into the waters urbanized coastal estuarine areas through fossil fuel spills, domestic industrial waste discharges, atmospheric inputs, continental runoff poses a major threat to fauna flora aquatic environment can have significant impact on internal defense system invertebrates mussels. Using monoclonal polyclonal anti-Toll-like receptor 2 (TLR2) anti-inducible nitric oxide synthetase (iNOS) antibodies for first time, this work aims examine hemocytes in mantle gills M. galloprovincialis biomarkers B[a]P pollution analyze their potential synergistic effect. To pursue objective, samples were exposed both individually simultaneously. Subsequently, oxidative stress evaluated by enzymatic analysis, while tissue changes number different contaminated groups assessed via histomorphological immunohistochemical analyses. Our findings revealed that comparison single exposure, two pollutants together significantly elevated stress. Moreover, our data may potentially enhance knowledge how TLR2 iNOS part bivalves. This would help creating new technologies strategies, biosensors, are more suitable managing water pollution, garnering details condition marine ecosystem.

Language: Английский

Citations

4
Evolution of snake skin: Role of cutaneous tactile corpuscles in Hierophis viridiflavus (Lacépède, 1789) DOI
Alessio Alesci, Sebastián Marino, Dario Di Fresco

et al.

Acta Zoologica, Journal Year: 2024, Volume and Issue: 106(1), P. 88 - 99

Published: March 18, 2024

Abstract The skin of snakes acts as the first interface between these reptiles and environment they live in. It plays an important role in ecology organisms, specialised structures, such tactile corpuscles, are crucial sensing a wide variety stimuli. This study, by examining presence, localisation colocalisation serotonin, substance P, PGP 9.5 tubulin, wants to demonstrate sensory that structures play Green whip snake Hierophis viridiflavus (Lacépède, 1789). study presents evaluation nerve immune molecules (TLR2) corpuscles H. viridiflavius ventral skin, indicating potential defence structures. These findings provide valuable information on snakes, their systems, evolutionary morphostructural adaptations. Additionally, identification could support for taxonomic recognition. Furthermore, this expands knowledge about stimuli snakes.

Language: Английский

Citations

1
Immunohistochemical Characterization of Langerhans Cells in the Skin of Three Amphibian Species DOI Creative Commons

Giorgia Pia Lombardo,

Anthea Miller,

Marialuisa Aragona

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(4), P. 210 - 210

Published: March 23, 2024

The amphibian taxon includes three orders that present different morphological characteristics: Anura, Caudata, and Apoda. Their skin has a crucial role: it acts as an immune organ constituting physical, chemical, immunological, microbiological barrier to pathogen insult conducts essential physiological processes. Amphibians have developed specialized features protect the vulnerable barrier, including glandular network beneath surface can produce antimicrobial toxic substances, thus contributing defense against pathogens predators. This study aims characterize Langerhans cells in of Lithobates catesbeianus (order: Anura; Shaw, 1802), Amphiuma means Caudata; Garden, 1821), Typhlonectes natans Apoda; Fischer, 1880) with following antibodies: Langerin/CD207 (c-type lectin), Major Histocompatibility Complex (MHC)II, Toll-like receptor (TLR)2 (expressed by types DCs). Our results showed positive for Langerin CD/207 epidermis species; moreover, some antigen-presenting (APCs) connective tissue expressed TLR2 MHCII. distribution is very similar amphibians examined, despite their habitats. A greater knowledge system could be useful better understand phylogeny vertebrates safeguard from population declines. Furthermore, similarities between amphibians’ human concerning immunological may both biology translational medicine.

Language: Английский

Citations

1