Biomaterials in Tendon and Skeletal Muscle Tissue Engineering: Current Trends and Challenges

Materials, Journal Year: 2018, Volume and Issue: 11(7), P. 1116 - 1116

Published: June 29, 2018

Tissue engineering is a promising approach to repair tendon and muscle when natural healing fails. Biohybrid constructs obtained after cells’ seeding culture in dedicated scaffolds have indeed been considered as relevant tools for mimicking native tissue, leading better integration vivo. They can also be employed perform advanced vitro studies model the cell differentiation or regeneration processes. In this review, we report analyze different solutions proposed literature, reconstruction of tendon, muscle, myotendinous junction. classically rely on three pillars tissue engineering, i.e., cells, biomaterials environment (both chemical physical stimuli). We chosen present biomimetic bioinspired strategies based understanding structure/functions/properties interest. For each sorted publications according an increasing degree complexity materials’ shape manufacture. their biological mechanical performances, observed vivo available. Although there no consensus gold standard technique reconstruct these musculo-skeletal tissues, reader find ways progress field understand recent history choice materials, from collagen polymer-based matrices.

Language: Английский

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Tendon Tissue Engineering: Effects of Mechanical and Biochemical Stimulation on Stem Cell Alignment on Cell‐Laden Hydrogel Yarns

Advanced Healthcare Materials, Journal Year: 2019, Volume and Issue: 8(7)

Published: Feb. 6, 2019

Fiber-based approaches hold great promise for tendon tissue engineering enabling the possibility of manufacturing aligned hydrogel filaments that can guide collagen fiber orientation, thereby providing a biomimetic micro-environment cell attachment, migration, and proliferation. In this study, 3D system composed cell-laden, highly yarns is designed obtained via wet spinning in order to reproduce morphology structure fascicles. A bioink alginate gelatin methacryloyl (GelMA) optimized loaded with human bone morrow mesenchymal stem cells (hBM-MSCs). The produced scaffolds are subjected mechanical stretching recapitulate strains occurring native tissue. Stem differentiation promoted by addition morphogenetic protein 12 (BMP-12) culture medium. orientation fibers combined stimulation results preferential longitudinal demonstrates enhanced type I III expression. Additionally, combination biochemical stimulations promotes expression specific tenogenic markers, signatures efficient towards tendon. suggest proposed cell-laden be used regeneration.

Language: Английский

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Flexible bipolar nanofibrous membranes for improving gradient microstructure in tendon-to-bone healing

Acta Biomaterialia, Journal Year: 2017, Volume and Issue: 61, P. 204 - 216

Published: Aug. 1, 2017

Language: Английский

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Anisotropic Hybrid Hydrogels with Superior Mechanical Properties Reminiscent of Tendons or Ligaments

Advanced Functional Materials, Journal Year: 2019, Volume and Issue: 29(38)

Published: Aug. 6, 2019

Abstract Mimicking the hierarchically anisotropic structure and excellent mechanical properties of natural tissues, such as tendons ligaments, using biomaterials is challenging. Despite recent achievements with hydrogels, limitations remain because difficulties in achieving both structural characteristics simultaneously. A simple approach for fabricating hybrid hydrogels a superior that are reminiscent or ligaments proposed. Alginate–polyacrylamide double‐network (DN) incorporated high aspect ratio mesoporous silica microparticles stretched fixed via subsequent drying ionic crosslinking to achieve multiscale structures composed an anisotropically aligned polymer network embedded microparticles. The can be further controlled by degree stretching, quantities, functional groups inorganic microparticles, types cations. reswelling results water content (>80%) similar while strength, modulus, toughness maintained. optimized hydrogel exhibits tensile modulus 7.2 MPa, strength 1.3 1.4 MJ m −3 even swollen state, which 451‐, 27‐, 2.2 times higher than observed non‐swollen tough DN hydrogel. This study suggests new strategy develop artificial ligaments.

Language: Английский

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Mechanics of bioinspired functionally graded soft-hard composites made by multi-material 3D printing

Composite Structures, Journal Year: 2020, Volume and Issue: 237, P. 111867 - 111867

Published: Jan. 6, 2020

Language: Английский

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Biomimetic strategies for tendon/ligament-to-bone interface regeneration

Bioactive Materials, Journal Year: 2021, Volume and Issue: 6(8), P. 2491 - 2510

Published: Feb. 6, 2021

Tendon/ligament-to-bone healing poses a formidable clinical challenge due to the complex structure, composition, cell population and mechanics of interface. With rapid advances in tissue engineering, variety strategies including advanced biomaterials, bioactive growth factors multiple stem lineages have been developed facilitate this Given important role structure-function relationship, review begins with brief description enthesis structure composition. Next, biomimetic biomaterials decellularized extracellular matrix scaffolds synthetic-/natural-origin are critically examined. Then, key roles combination, concentration location various application emphasized. After that, sources culture systems described. At last, we discuss unmet needs existing challenges ideal for tendon/ligament-to-bone regeneration highlight emerging field.

Language: Английский

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Heparin functionalization increases retention of TGF-β2 and GDF5 on biphasic silk fibroin scaffolds for tendon/ligament-to-bone tissue engineering

Acta Biomaterialia, Journal Year: 2018, Volume and Issue: 72, P. 150 - 166

Published: March 18, 2018

Language: Английский

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Electrospun thymosin Beta-4 loaded PLGA/PLA nanofiber/ microfiber hybrid yarns for tendon tissue engineering application

Materials Science and Engineering C, Journal Year: 2019, Volume and Issue: 106, P. 110268 - 110268

Published: Oct. 12, 2019

Language: Английский

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Biotherapeutic-loaded injectable hydrogels as a synergistic strategy to support myocardial repair after myocardial infarction

Journal of Controlled Release, Journal Year: 2021, Volume and Issue: 335, P. 216 - 236

Published: May 19, 2021

Language: Английский

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^{Fabrication and Characterization of Biphasic Silk Fibroin Scaffolds for Tendon/Ligament-to-Bone Tissue Engineering}

Tissue Engineering Part A, Journal Year: 2017, Volume and Issue: 23(15-16), P. 859 - 872

Published: March 23, 2017

Tissue engineering is an attractive strategy for tendon/ligament-to-bone interface repair. The structure and extracellular matrix composition of the are complex allow a gradual mechanical stress transfer between tendons/ligaments bone. Thus, scaffolds mimicking structural features native may be able to better support functional tissue regeneration. In this study, we fabricated biphasic silk fibroin designed mimic gradient in collagen molecule alignment present at interface. had two different pore alignments: anisotropic tendon/ligament side isotropic bone side. Total porosity ranged from 50% 80% majority pores (80-90%) were <100-300 μm. Young's modulus varied 689 1322 kPa depending on type construct. addition, human adipose-derived mesenchymal stem cells cultured evaluate effect morphology cell proliferation gene expression. Biphasic supported attachment influenced cytoskeleton organization alignment. expression tendon/ligament, enthesis, cartilage markers significantly changed each region scaffolds. conclusion, study show promising engineering.

Language: Английский

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