Frontiers in Bioengineering and Biotechnology,
Journal Year:
2024,
Volume and Issue:
12
Published: Aug. 14, 2024
Natural
biomaterials,
particularly
fibrous
proteins,
are
extensively
utilized
in
skin
tissue
engineering.
However,
their
application
is
impeded
by
batch-to-batch
variance,
limited
chemical
or
physical
versatility,
and
environmental
concerns.
Recent
advancements
gene
editing
fermentation
technology
have
catalyzed
the
emergence
of
recombinant
protein
which
gaining
traction
The
modular
highly
customizable
nature
synthesis
enables
precise
control
over
biomaterial
design,
facilitating
incorporation
multiple
functional
motifs.
Additionally,
allows
for
a
transition
from
animal-derived
sources
to
microbial
sources,
thereby
reducing
endotoxin
content
rendering
biomaterials
more
amenable
scalable
production
clinical
use.
In
this
review,
we
provide
an
overview
prevalent
(collagens,
elastin,
silk
proteins
chimeric
derivatives)
used
engineering
(STE)
compare
them
with
counterparts.
Furthermore,
discuss
applications
STE,
along
associated
challenges
future
prospects.
Biomaterials and Biosystems,
Journal Year:
2024,
Volume and Issue:
16, P. 100102 - 100102
Published: Oct. 10, 2024
There
is
an
increasing
demand
to
not
only
accelerate
the
development
of
advanced
therapy
tissue
engineered
medicines,
but
also
eliminate
xenogeneic
materials
from
their
cycle.
With
these
in
mind,
herein
we
first
assessed
influence
carrageenan
as
macromolecular
crowding
agent
enhance
and
extracellular
matrix
deposition
xeno-free
human
umbilical
cord
mesenchymal
stromal
cell
cultures
developed
characterised
a
non-animal
sourced
chitosan
scaffold.
Following
appropriate
vitro
experimentation,
splinted
nude
mouse
wound
healing
model
was
used
assess
closure
scar
size
non-treated
control,
scaffold,
scaffold
loaded
with
cells
cultured
under
conditions
groups.
Across
all
three
donors,
supplementation
significantly
increased
collagen
at
day
5,
8
11
without
affecting
morphology,
viability,
DNA
concentration
metabolic
activity.
Through
freeze
drying,
sponge
structural
mechanical
properties
for
applications.
In
biological
analysis
made
apparent
that
neither
nor
negatively
impacted
activity
proliferation.
vivo
revealed
no
significant
differences
between
groups
size,
raising
question
about
suitability
model.
any
case,
this
work
sets
foundations
completely
medicines.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: May 10, 2024
Abstract
Hydrogel
biomaterials
offer
great
promise
for
3D
cell
culture
and
therapeutic
delivery.
Despite
many
successes,
challenges
persist
in
that
gels
formed
from
natural
proteins
are
only
marginally
tunable
while
those
derived
synthetic
polymers
lack
intrinsic
bioinstructivity.
Towards
the
creation
of
with
both
excellent
biocompatibility
customizability,
recombinant
protein-based
hydrogels
have
emerged
as
molecularly
defined
user-programmable
platforms
mimic
proteinaceous
nature
extracellular
matrix.
Here,
we
introduce
PhoCoil,
a
dynamically
hydrogel
single
protein
component
unique
multi-stimuli
responsiveness.
Physical
crosslinking
through
coiled-coil
interactions
promotes
rapid
shear-thinning
self-healing
behavior,
rendering
gel
injectable,
an
included
photodegradable
motif
affords
on-demand
network
dissolution
via
visible
light.
PhoCoil
photodegradation
can
be
spatiotemporally
lithographically
controlled
dose-dependent
manner,
complex
tissue,
without
harm
to
encapsulated
cells.
We
anticipate
will
enable
new
applications
tissue
engineering
regenerative
medicine.
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2024,
Volume and Issue:
12
Published: Aug. 14, 2024
Natural
biomaterials,
particularly
fibrous
proteins,
are
extensively
utilized
in
skin
tissue
engineering.
However,
their
application
is
impeded
by
batch-to-batch
variance,
limited
chemical
or
physical
versatility,
and
environmental
concerns.
Recent
advancements
gene
editing
fermentation
technology
have
catalyzed
the
emergence
of
recombinant
protein
which
gaining
traction
The
modular
highly
customizable
nature
synthesis
enables
precise
control
over
biomaterial
design,
facilitating
incorporation
multiple
functional
motifs.
Additionally,
allows
for
a
transition
from
animal-derived
sources
to
microbial
sources,
thereby
reducing
endotoxin
content
rendering
biomaterials
more
amenable
scalable
production
clinical
use.
In
this
review,
we
provide
an
overview
prevalent
(collagens,
elastin,
silk
proteins
chimeric
derivatives)
used
engineering
(STE)
compare
them
with
counterparts.
Furthermore,
discuss
applications
STE,
along
associated
challenges
future
prospects.
