Journal of Plant Physiology, Journal Year: 2024, Volume and Issue: 302, P. 154324 - 154324
Published: Aug. 6, 2024
Language: Английский
British Journal of Haematology, Journal Year: 2025, Volume and Issue: 206(2), P. 628 - 643
Published: Jan. 6, 2025
Summary Chimeric antigen receptor T‐cell (CAR‐T) therapy has shown transformative potential in treating malignant tumours, with increasing global approval of CAR‐T products. However, high‐production costs and risks associated viral vector‐based cells—such as insertional mutagenesis secondary tumour formation—remain challenges. Our study introduces an innovative engineering approach using mRNA delivered via lipid nanoparticles (LNPs), aiming to reduce enhance safety while maintaining strong anti‐tumour efficacy. We developed LNP‐based transfection protocol for efficient delivery encoding full‐human CAR constructs, achieving high expression significant cytotoxicity against leukaemic cells vitro. Co‐culture Raji showed increased cytokine secretion cell killing by mRNA‐LNP cells. Therapeutic efficacy was further demonstrated NOD‐scid‐IL2Rγnull (NSG) mouse model engraftment, where treated mice exhibited marked regression extended survival. These findings underscore the mRNA‐LNPs a non‐viral, effective platform, offering promising alternative traditional methods that could improve safety, accessibility clinical cancer immunotherapy.
Language: Английский
Citations
1
MedComm, Journal Year: 2025, Volume and Issue: 6(2)
Published: Feb. 1, 2025
Abstract Circular RNA (circRNA), a subtype of noncoding RNA, has emerged as significant focus in research due to its distinctive covalently closed loop structure. CircRNAs play pivotal roles diverse physiological and pathological processes, functioning through mechanisms such miRNAs or proteins sponging, regulation splicing gene expression, serving translation templates, particularly the context various cancers. The hallmarks cancer comprise functional capabilities acquired during carcinogenesis tumor progression, providing conceptual framework that elucidates nature malignant transformation. Although numerous studies have elucidated role circRNAs cancers, their functions development chemoradiotherapy resistance remain unexplored clinical applications circRNA‐based translational therapeutics are still infancy. This review provides comprehensive overview circRNAs, covering biogenesis, unique characteristics, functions, turnover mechanisms. We also summarize involvement relevance biomarkers therapeutic targets, especially thyroid (TC). Considering potential fascination therapeutics, “Ying‐Yang” dynamic regulations TC warrant vastly dedicated investigations.
Language: Английский
Citations
1
Genes & Diseases, Journal Year: 2025, Volume and Issue: unknown, P. 101605 - 101605
Published: March 1, 2025
Language: Английский
Citations
1
Advanced Materials, Journal Year: 2025, Volume and Issue: unknown
Published: March 27, 2025
Ischemic stroke represents one of the leading cerebrovascular diseases with a high rate mortality and disability globally. To date, there are no effective clinical drugs available to improve long-term outcomes for post-stroke patients. A novel nucleic acid agent circSCMH1 which can promote sensorimotor function recovery in rodent nonhuman primate animal models has been found. However, still delivery challenges overcome its implementation. Besides, effects on cognitive functions remain unexplored. Herein, lipid nanoparticle circSCMH1@LNP1 is established deliver explore therapeutic efficacy comprehensively. Distribution experiments demonstrate that intranasal administration significantly increases distribution peri-infarct region reduces non-specific accumulation other organs compared intravenous injection. Therapeutic results indicate promotes synaptic plasticity, vascular repair, neuroinflammation relief, myelin sheath formation, thereby achieving enhanced mice. In conclusion, this research presents simple LNP system efficient via repair brain injury. It envisioned study may bridge crucial gap between basic translational application, paving way implementation patient management.
Language: Английский
Citations
1
Journal of Plant Physiology, Journal Year: 2024, Volume and Issue: 302, P. 154324 - 154324
Published: Aug. 6, 2024
Language: Английский
Citations
6