Surface modified proteins and peptides for targeted drug delivery

Progress in molecular biology and translational science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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Microneedle-delivered adeno-associated virus vaccine amplified anti-viral immunity by improving antigen-presenting cells infection

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: 379, P. 1045 - 1057

Published: Feb. 5, 2025

Language: Английский

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Potential of Encapsulated Bovine Colostrum in Powder-Based Formulations for Facial Clay, Peel-Off Gel, and Sleeping Gel Masks

Gels, Journal Year: 2025, Volume and Issue: 11(2), P. 111 - 111

Published: Feb. 4, 2025

Bovine colostrum is a bioactive compound with potential in cosmetic applications but has limited shelf life. This study aimed to develop an effective encapsulation system for bovine using the complex coacervation method and incorporate it into powder formulations facial masks. The research explored various gelatin-to-gum Arabic ratios optimize physical chemical stability, efficiency, loading capacity of encapsulated (EBC). EBC was further incorporated clay masks, peel-off gel sleeping optimal ratio found be 2:1, yielding highest entrapment efficiency (66.6 ± 3.3% w/w) (67.6 3.4% colostrum. For most blend incorporating enhanced moisture content, water solubility, hygroscopicity, without affecting drying time (9.7 0.6 min). Additionally, masks significantly reduced activity improved content while decreased from 44.3 25.0 1.7 min. formulation increased activity, other parameters remained stable. In conclusion, stability effectively integrated powders mask formulations.

Language: Английский

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Sustained Delivery of Liraglutide Using Multivesicular Liposome Based on Mixed Phospholipids

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(2), P. 203 - 203

Published: Feb. 6, 2025

Background: Although peptides are widely used in the clinical treatment of various diseases due to their strong biological activity, they usually require frequent injections owing poor vivo half-life. Therefore, there is a need for sustained peptide formulations. Methods: In this study, liraglutide (Lir) and biocompatible multivesicular liposomes (MVLs) were utilized as model drug sustained-release carriers, respectively. The release rate Lir-MVLs was controlled by changing ratio SPC DEPC with different phase transition temperatures (PTT, PTTSPC = −20 °C, PTTDEPC 13 °C). Results: As increased, had more flexible lipid membranes, poorer structural stabilization, fewer internal vesicles larger particle sizes, contributing faster Lir. After subcutaneous injection Lir-MVLs, blood glucose concentration (BGC) db/db mice decreased levels. When SPC-DEPC greater than 85:15, too fast; BGC remained below 16 mM only 2–4 days, while when slow, case less 50:50, also 2–3 days. However, 75:25, could be maintained 8 indicating that properties best met pharmacological requirements Conclusions: This study investigated effects phospholipids PTT on characteristics provided ideas design preparations.

Language: Английский

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Biopharmaceutical Analysis by HPLC: Practices and Challenges

Chonnam Medical Journal, Journal Year: 2025, Volume and Issue: 61(1), P. 19 - 19

Published: Jan. 1, 2025

High-Performance Liquid Chromatography (HPLC) is an essential analytical technique in the biopharmaceutical industry, crucial for separation, identification, and quantification of complex biological molecules such as monoclonal antibodies recombinant proteins. It plays a vital role assessing purity, potency, stability products, which are critical regulatory approval. HPLC offers high resolution sensitivity, allowing detection small quantities compounds samples. Its versatility evident various modes, including reversed-phase, ion-exchange, size-exclusion, affinity chromatography. However, challenges remain, selecting appropriate stationary phase, addressing peak overlapping matrix interference, optimizing operational parameters like flow rate mobile phase composition. Standardization method validation ensuring reproducibility, accuracy, compliance analyses. The need reliable reference materials calibration methods also significant challenge. Recent advancements technology, ultra-high-performance liquid chromatography (UHPLC) hybrid systems that integrate with mass spectrometry, helping to overcome these by enhancing resolution, analysis speed. In summary, products grow more complex, HPLC's will continue evolve, highlighting ongoing research development refine this tool.

Language: Английский

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Peptide-driven strategies against lung cancer

Life Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 123453 - 123453

Published: Feb. 1, 2025

Language: Английский

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Dental plaque-inspired peptide engineered to control plaque accumulation

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101570 - 101570

Published: Feb. 8, 2025

Effective control of plaque accumulation is an important strategy for reducing the risk both localized oral health issues and systemic diseases associated with plaque. However, existing approaches preventing exhibit some limitations, such as insufficient compatibility microbiota tissues, well inconvenience in their use. Herein, inspired by dental plaque, a new class peptides featuring excellent anti-fouling performance successfully developed. Our consist salivary-acquired peptide tooth surface-selective adhesion, zwitterionic anti-adhesion property, four proline residues that provide structural rigidity. We conduct series progressive experiments, including molecular dynamics simulation assessments our on hydroxyapatite slices, human enamel ex vivo teeth. The results demonstrate possess abilities rapid anchoring surfaces effective inhibiting protein bacterial adhesion. These characteristics enable to efficiently through rinsing or spraying while preserving balance microbiota. findings open appealing avenue development agents controlling surfaces.

Language: Английский

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Microneedle Delivery of Protein and Peptides: Advances in Drug Delivery

Journal of Drug Delivery and Therapeutics, Journal Year: 2025, Volume and Issue: 15(2), P. 86 - 94

Published: Feb. 15, 2025

Microneedles are the advances in transdermal drug delivery system of proteins and peptide drugs which exerts its effect through formation micro channels. various types such as solid microneedles, dissolving metallic 3D printed microneedles; polymer based Hydrogel microneedles coated microneedles. Microneedle characterization is most important part after formulation. These dosage forms have both advantages limitations. Stability enhancement, targeted delivery, low invasiveness, enhanced skin absorption etc. some associated with Likewise shallow penetration, chances irritation injury, degradation different extreme temperature pH Needle integrity, uniform distribution, diffusion degradation, sterility contamination, immunogenicity immune response, Activity preservation crucial parts that should be controlled during Improved stability proteins, stabilization inactive ingredients, utilization field gene therapy mRNA development smart multilayered ligand targeting microneedle system. systems widely recognized future addressing challenges patient compliance. Keywords: Microneedles, Integrity, Immunogenicity, Patient compliance

Language: Английский

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Composite porous biopolymer multilayer films as potential controlled delivery systems for tolfenamic acid

Journal of Physics Conference Series, Journal Year: 2025, Volume and Issue: 2952(1), P. 012017 - 012017

Published: Feb. 1, 2025

Abstract The aim of this paper is to investigate the potential composite porous biopolymer films, created by lyophilization solutions two different biopolymers (Poly-D-lactic acid/PLA and Poly-ɛ-caprolactone/PEC) at mass ratios (pure PLA, 3-to-1, 1-to-1, 1-to-3 pure PEC), for controlled release a chosen bioactive substance (tolfenamic acid). For purpose, prepared films were charged under corona discharge in standard triode system (consisting charging electrode/needle supplied with 5 kV polarity, grid 1 same polarity as grounded electrode) positive charges. A set number chitosan casein multilayers deposited on surface using layer-by-layer (LbL) deposition technique, material being loaded layers. drug kinetics total amount released dissolution medium determined use spectrometer.

Language: Английский

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Enhanced Stability and Reusability of Subtilisin Carlsberg Through Immobilization on Magnetic Nanoparticles

Nanotechnology Science and Applications, Journal Year: 2025, Volume and Issue: Volume 18, P. 71 - 91

Published: Feb. 1, 2025

Immobilizing enzymes on solid supports such as magnetic nanoparticles offers multi-dimensional advantages, including enhanced conformational, structural, and thermal stability for long-term storage reusability. The gene encoding subtilisin Carlsberg was isolated from proteolytic Bacillus haynesii, a bacterium derived salt mines. nucleotide sequence pro-peptide mature protein were cloned into pET22(a)+ vector expressed in E. coli. extracted enzyme subsequently immobilized glutaraldehyde-linked-chitosan-coated nanoparticles. Fourier-transform infrared analysis revealed higher intensity peaks the enzyme-immobilized indicating an increase bonding numbers. X-ray diffraction mild amorphous state contrast to more crystalline free An increased mass content atomic percentage carbon nitrogen recorded EDX Dynamic light scattering showed average particle size ~85 nm ~250 nm. Upon immobilization, Michaelis-Menten value 11.5 mm 15.02 mM, while maximum velocity 13 mm/min 22.7 mm/min. Immobilization significantly improved thermostability with 75% activity retained by at 70 °C compared 50% same temperature. yield, efficiency recovery 61%, 84% 51%, respectively. 70% of its after 10 cycles reuse, it maintained 55% 30 days storage. present study highlights efficacy nanoparticle-based immobilization enhancing functioning facilitates incorporation commercial applications necessitating high reusability, detergents, medicines, bioremediation.

Language: Английский

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