BioNanoScience, Journal Year: 2025, Volume and Issue: 15(2)
Published: April 10, 2025
Language: Английский
Current Microbiology, Journal Year: 2025, Volume and Issue: 82(4)
Published: March 5, 2025
Language: Английский
Citations
0
Future Microbiology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 12
Published: March 22, 2025
Aims Candidiasis, caused by Candida spp. is an opportunistic infection with significant healthcare risks, worsened trends in antifungal resistance. This study aimed to evaluate the susceptibility profile, investigate resistance mechanisms, assess efflux pump activity, and examine biocide tolerance clinical isolates.
Language: Английский
Citations
0
Life, Journal Year: 2025, Volume and Issue: 15(4), P. 518 - 518
Published: March 21, 2025
Motivated by the search for novel antimicrobials against opportunistic resistant pathogens and based on reported antimicrobial activity of phthalimides, two series phthalimide N-phthaloylglycine esters were designed to investigate whether addition butyl aryl groups enhances their properties. Thus, in vitro activity, antifungal mechanism action, effect combined with Chloramphenicol, silico/vitro toxicity, a docking molecular studied. Phthalimide obtained yields 75–98%. ester 3b (R = Me) showed best results Gram-(+) Gram-(−) bacteria, S. aureus P. aeruginosa, respectively, yeast fungi, C. tropicalis albicans, MIC values equal 128 µg·mL−1. Regarding action compound changed from 1024 µg·mL−1 presence ergosterol. Furthermore, synergy Chloramphenicol FICI value 0.5. Finally, four most promising compounds had toxicity evaluated, which moderate non-toxicity Artemia salina larvae. With exception all selected compounds, including Fluconazole, are potentially hepatotoxic, but they predicted not cause skin sensitization, suggesting potential application topical use. Molecular revealed that exhibits superior binding affinity stability 50S ribosomal subunit (−92.69 kcal·mol−1) compared unique π–sulfur interaction CYP51, its as dual-action antibacterial candidate pathogens.
Language: Английский
Citations
0
Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown
Published: March 31, 2025
ABSTRACT Invasive candidiasis has emerged as a significant healthcare challenge, with rising incidence rate attributed to the widespread use of organ transplantation, chemotherapy, immunosuppressants, and broad-spectrum antibiotics. The increasing prevalence drug-resistant strains, particularly among Candida tropicalis , necessitated exploration novel therapeutic strategies. Our study investigated synergistic effects minocycline (MIN) combined fluconazole (FLC) against FLC-resistant C. both in vitro vivo . activity MIN FLC was evaluated using checkerboard titration time-kill assays. Galleria mellonella larvae mouse model were employed assess efficacy, histopathological examination fungal burden quantification. Whole-genome RNA sequencing elucidated mechanisms observed. FLC/MIN combination significantly lowered minimum inhibitory concentration (MIC) improved fungicidal activity, evidenced by enhanced survival rates reduced G. models. Histopathological analysis confirmed less tissue damage load therapy. suggested that impact on amino acid metabolism contributes effects. This approach holds promise for treating antifungal efficacy reducing drug resistance risks, warranting further clinical exploration. IMPORTANCE highlights potential therapy combating It shows promising effects, MIC enhancing activity. Further trials are needed validate its infections.
Language: Английский
Citations
0
BioNanoScience, Journal Year: 2025, Volume and Issue: 15(2)
Published: April 10, 2025
Language: Английский
Citations
0