The Journal of Gene Medicine,
Journal Year:
2024,
Volume and Issue:
26(8)
Published: Aug. 1, 2024
Abstract
Introduction
Esophageal
cancer
is
one
of
the
major
cancers
in
China.
Most
patients
with
esophageal
are
diagnosed
at
an
advanced
stage,
and
5
year
survival
rate
discouraging.
Combined
chemotherapy
a
common
method
for
treatment
cancer.
Methods
In
this
study,
distearoyl
phosphatidyl
ethanolamine
polyethylene
glycol
2000
(DSPE‐PEG2000)
nanoliposomes
(NLPs)
encapsulating
anticancer
drugs
docetaxel
(DOX)
oridonin
(ORD)
were
prepared,
their
ability
to
enhance
release
was
determined.
The
NLP
system
characterized
by
transmission
electron
microscopy,
particle
size
encapsulation
efficiency.
addition,
characteristics
pharmacodynamics
these
also
studied
detail.
Results
When
DOX/ORD
ratio
2:1,
higher
proportion
DOX
led
stronger
synergy
effect.
NLPs
prepared
high‐pressure
homogenization
had
uniform
spherical
morphology.
mean
polydispersity
index
determined
be
246.4
0.163,
respectively.
stability
results
showed
that
no
significant
change
observed
size,
zeta
potential,
Encapsulation
efficiency
dynamic
light
scattering
during
observation
period.
vitro
illustrated
acidic
environment
tumor
might
beneficial
drug
release.
three‐dimensional
tumorsphere
can
reach
interior
spheres,
which
destroys
structure
cells,
resulting
irregular
spheres.
vivo
study
indicated
obvious
targeting
effect
on
subcutaneous
tumors
have
potential
actively
deliver
tissues.
Terminal
deoxynucleotidyl
transferase
dUTP
nick
end
labeling
(TUNEL)
staining
used
detect
apoptosis.
could
significantly
induce
apoptosis
inhibit
growth.
Conclusion
anti‐tumor
activity,
expected
promising
co‐delivery
platform
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: April 18, 2024
Abstract
Lipid
nanoparticles
(LNPs)
have
emerged
as
the
dominant
platform
for
RNA
delivery,
based
on
their
success
in
COVID-19
vaccines
and
late-stage
clinical
studies
other
indications.
However,
we
others
shown
that
LNPs
induce
severe
inflammation,
massively
aggravate
pre-existing
inflammation.
Here,
using
structure-function
screening
of
lipids
analyses
signaling
pathways,
elucidate
mechanisms
LNP-associated
inflammation
demonstrate
solutions.
We
show
LNPs’
hallmark
feature,
endosomal
escape,
which
is
necessary
expression,
also
directly
triggers
by
causing
membrane
damage.
Large,
irreparable,
holes
are
recognized
cytosolic
proteins
called
galectins,
bind
to
sugars
inner
then
regulate
downstream
find
inhibition
galectins
abrogates
both
vitro
vivo
.
rapidly
biodegradable
ionizable
can
preferentially
create
smaller
size
reparable
sorting
complex
required
transport
(ESCRT)
pathway.
Ionizable
producing
such
ESCRT-recruiting
produce
high
expression
from
cargo
mRNA
with
minimal
Finally,
routes
non-inflammatory
LNPs,
either
galectin
or
lipids,
compatible
therapeutic
mRNAs
ameliorate
disease
models.
without
lead
exacerbation
these
In
summary,
escape
induces
damage
be
controlled
inhibiting
(large
hole
detectors)
ESCRT
These
strategies
should
generally
safer
used
treat
inflammatory
diseases.
Molecular Cancer,
Journal Year:
2024,
Volume and Issue:
23(1)
Published: Aug. 30, 2024
Drug
resistance
in
cancer
cells
significantly
diminishes
treatment
efficacy,
leading
to
recurrence
and
metastasis.
A
critical
factor
contributing
this
is
the
epigenetic
alteration
of
gene
expression
via
RNA
modifications,
such
as
N6-methyladenosine
(m6A),
N1-methyladenosine
(m1A),
5-methylcytosine
(m5C),
7-methylguanosine
(m7G),
pseudouridine
(Ψ),
adenosine-to-inosine
(A-to-I)
editing.
These
modifications
are
pivotal
regulating
splicing,
translation,
transport,
degradation,
stability.
Governed
by
"writers,"
"readers,"
"erasers,"
impact
numerous
biological
processes
progression,
including
cell
proliferation,
stemness,
autophagy,
invasion,
apoptosis.
Aberrant
can
lead
drug
adverse
outcomes
various
cancers.
Thus,
targeting
modification
regulators
offers
a
promising
strategy
for
overcoming
enhancing
efficacy.
This
review
consolidates
recent
research
on
role
prevalent
resistance,
with
focus
m6A,
m1A,
m5C,
m7G,
Ψ,
A-to-I
Additionally,
it
examines
regulatory
mechanisms
linked
underscores
existing
limitations
field.
ACS Nano,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 6, 2025
Lipid
nanoparticles
(LNPs)
have
emerged
as
a
groundbreaking
delivery
system
for
vaccines
and
therapeutic
mRNAs.
Ionizable
lipids
are
the
most
pivotal
component
of
LNPs
due
to
their
ability
electrostatically
interact
with
mRNA,
allowing
its
encapsulation
while
concurrently
enabling
endosomal
escape
following
cellular
internalization.
Thus,
extensive
research
has
been
performed
optimize
ionizable
lipid
structure
develop
formulations
that
well
tolerated
allow
efficient
targeting
different
organs
result
in
high
sustained
mRNA
expression.
However,
one
facet
lipids'
mostly
overlooked:
linker
segment
between
headgroup
tails.
Here,
we
screened
rationally
designed
library
biodegradable
linkers.
We
extensively
characterized
formulated
using
these
elucidated
how
minor
structural
changes
radically
influenced
LNPs'
biodistribution
vivo.
showed
use
amide
urea
linkers
can
modulate
pKa,
resulting
an
improved
specificity
lung
transfection.
Finally,
demonstrated
(lipid
35)
form
entrapping
bacterial
toxin
[pseudomonas
exotoxin
A
(mmPE)]
reduced
tumor
burden
significantly
increased
survival
mice
metastasis.
Research Square (Research Square),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 10, 2025
Abstract
mRNA
vaccines
have
shown
great
efficacy
against
SARS-CoV-2,
yet
challenges
remain
in
optimizing
vaccine
components
to
achieve
enhanced
immune
response
and
stability.
In
this
study,
we
developed
CPVax-CoV,
a
new
lyophilized
that
features
novel
thiolactone-based
ionizable
lipids
newly
designed
untranslated
regions
(UTRs)
for
expression.
Incorporation
of
these
optimized
into
our
candidate
CPVax-CoV
significantly
improved
responses
mice
compared
commercially
available
vaccines.
Moreover,
has
proven
be
thermostable,
maintaining
its
biological
activity
up
one
year
at
4°C
25°C
after
lyophilization,
overcoming
the
cold-chain
limitations
current
This
demonstrates
protective
ancestral
SARS-CoV-2
Omicron
XBB
variant,
offering
scalable
solution
global
distribution
pandemic
preparedness.
These
findings
underscore
potential
platform
future
next-generation
development.
Dentistry Journal,
Journal Year:
2025,
Volume and Issue:
13(2), P. 79 - 79
Published: Feb. 13, 2025
Background:
Recent
advances
in
mRNA
vaccine
technology,
accelerated
by
the
global
COVID-19
pandemic,
have
generated
significant
interest
their
applications
beyond
infectious
diseases.
Dentistry
has
emerged
as
a
promising
field
for
exploring
potential
of
mRNA-based
therapies
preventing
and
treating
oral
Objectives:
This
narrative
review
aims
to
evaluate
current
status
development
its
preclinical
health,
focusing
on
periodontal
disease,
dental
caries,
regenerative
medicine,
implantology,
cancer.
Methods:
The
synthesizes
findings
from
studies,
including
research
conducted
animal
models
vitro,
assess
modulate
immune
responses
promote
tissue
regeneration
cavity.
Clinical
trials
were
only
mentioned
context
broader
areas
implementation
such
oncology
immunotherapy.
Results:
studies
highlight
capacity
vaccines
enhance
body's
response
facilitate
repair
processes.
Despite
these
results,
challenges
persist
delivering
effectively
within
complex
environment.
These
include
stability,
delivery
mechanisms,
modulation
responses.
Conclusions:
While
offer
promise
revolutionizing
health
care,
they
face
notable
limitations
concerning
safety,
efficacy,
clinical
feasibility.
Overcoming
obstacles
through
further
is
essential
unlock
full
translational
ensure
safe
effective
integration
into
practice.
