Therapeutic potential of human mesenchymal stromal cell-derived mitochondria in a rat model of post-surgical digestive fistula: towards an energetic nano-biotherapy DOI Open Access
Antoine Mariani, A Guichard, Anna Sebbagh

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 3, 2024

Abstract Background Tissue regeneration heavily relies on cellular energy production, with mitochondria playing a crucial role. Dysfunctional are implicated in various degenerative diseases, driving interest targeting mitochondrial transplantation for tissue repair. Wound healing is highly compromised gastrointestinal conditions resulting fistula development, particularly after sleeve gastrectomy. Human mesenchymal stem/stromal cells (hMSCs) and their cell-free products such as offer potential benefits due to therapeutic properties production. Here we investigated the advantage of hMSCs-derived nano-biotherapy rat model post-surgical healing. Methods Viable structurally intact were isolated from hMSCs before exposure human colonic epithelial (HCEC-1CT) culture or into post-operative fistula. Results Our findings reveal significant dose-dependent improvement metabolic activity ATP content recipient cells. Assessment external orifice developed following post gastrectomy fistula, revealed substantial all transplanted rats compared control group. Conclusion highlight This research contributes advancing regenerative strategies conditions, offering new insights mitochondrial-based therapies enhancing wound

Language: Английский

A Phase 1, Open-label Study to determine Safety and Tolerability of the Topical Application of Mesenchymal stem/stromal cell (MSC) Exosome Ointment to Treat Psoriasis in Healthy Volunteers DOI Creative Commons
Nisha Suyien Chandran,

Monil Nagad Bhupendrabhai,

Thong Teck Tan

et al.

Cytotherapy, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

2
Pharmacokinetic characteristics of mesenchymal stem cells in translational challenges DOI Creative Commons
Yunlong Shan, Mengying Zhang,

Enxiang Tao

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)

Published: Sept. 13, 2024

Language: Английский

Citations

11
hAMSCs regulate EMT in the progression of experimental pulmonary fibrosis through delivering miR-181a-5p targeting TGFBR1 DOI Creative Commons
Yanyang Wang, Chan Liu, Nuoxin Wang

et al.

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 5, 2025

Pulmonary fibrosis (PF) is a common and multidimensional devastating interstitial lung disease. The development of novel more effective interventions for PF an urgent clinical need. A previous study has found that miR-181a-5p plays important role in the PF, human amniotic mesenchymal stem cells (hAMSCs) exert potent therapeutic potential on PF. However, whether hAMSCs act by delivering its detailed mechanism still remain unknown. Thus, this was designed to investigate underlying possible bleomycin (BLM)-induced mouse model, co-culture system A549 epithelial transition (EMT) focusing effects collagen deposition, EMT, cell cycle regulation. with different expression levels were constructed. BLM (4 mg/kg) used create while TGF-β1 induce construct EMT model. Furthermore, deposition during assessed vivo vitro. We exerted anti-fibrotic effect BLM-induced Moreover, also protective TGFβ1-induced ameliorated promoting proliferation, reducing apoptosis, attenuating through paracrine effects. regulated targeting TGFBR1. Our findings reveal first time inhibit EMT. Mechanistically, hMASCs achieved

Language: Английский

Citations

1
Surface-modified extracellular vesicles take their chance in the big PCSK9 inhibitors’ race DOI
Soumaya Ben‐Aicha, Costanza Emanueli

Molecular Therapy, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
In Vivo Reprogramming of Tissue‐Derived Extracellular Vesicles for Treating Chronic Tissue Injury Through Metabolic Engineering DOI Creative Commons
Meng Zhao, Shuyun Liu, Yizhuo Wang

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

Extracellular vesicles (EVs) have emerged as promising therapeutics for regenerative medicine, but the efficacy of current exogenous EV-based therapies treating chronic tissue injury is still unsatisfactory. Exercise can affect skeletal muscle EV secretion and that this process regulates systemic health-promoting role exercise, suggesting fine-tuning endogenous may provide a new therapeutic avenue. Here, work reports in vivo reprogramming via metabolic engineering strategy diseases. Briefly, exercise enhanced mitochondrial metabolism production healthy muscles, EVs from muscles subjected to or (boosting biogenesis AAV-mediated muscle-specific TFAM overexpression) exerted cellular protective effects vitro. In injured therapy could reprogram patterns (reducing pathological compositions while increasing beneficial compositions) by regulating multiple cargo sorting pathways. Reprogrammed muscle-derived reach major organs tissues circulation then simultaneously attenuated multiple-tissue (e.g., kidney) kidney disease. This study highlights tissue-derived approach potential diverse

Language: Английский

Citations

0
GelMA/HA-NB hydrogel encapsulating adipose-derived chondrogenic exosomes enhances enthesis regeneration in chronic rotator cuff tears DOI
Yan Xu, Xin Shi,

Haofeng Lin

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142800 - 142800

Published: April 1, 2025

Language: Английский

Citations

0
The bone microenvironment: new insights into the role of stem cells and cell communication in bone regeneration DOI Creative Commons
Luca Dalle Carbonare, Mattia Cominacini, Elisabetta Trabetti

et al.

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 12, 2025

Mesenchymal stem cells (MSCs) play a crucial role in bone formation and remodeling. Intrinsic genetic factors extrinsic environmental cues regulate their differentiation into osteoblasts. Within the microenvironment, complex network of biochemical biomechanical signals orchestrates homeostasis regeneration. In addition, crosstalk among MSCs, immune cells, neighboring cells-mediated by extracellular vesicles non-coding RNAs (such as circular micro RNAs) -profoundly influences osteogenic Recent studies have explored specific signaling pathways that contribute to effective regeneration, highlighting potential manipulating microenvironment enhance MSC functionality. The integration advanced biomaterials, gene editing techniques, controlled delivery systems is paving way for more targeted efficient regenerative therapies. Furthermore, artificial intelligence could improve tissue engineering, optimize biomaterial design, enable personalized treatment strategies. This review explores latest advancements emphasizing intricate interplay molecules. By providing comprehensive overview these mechanisms clinical implications, we aim shed light on future research directions this rapidly evolving field.

Language: Английский

Citations

0
Roles and Therapeutic Targeting of Exosomes in Sepsis‐Induced Cardiomyopathy DOI Creative Commons
Rui Fan, Han Liu,

Qun Liang

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2025, Volume and Issue: 29(8)

Published: April 1, 2025

ABSTRACT Sepsis‐induced cardiomyopathy (SICM) is a complex and fatal manifestation of sepsis, characterised by myocardial dysfunction that exacerbates the clinical prognosis in septic patients. While pathophysiology SICM remains incompletely understood, emerging evidence highlights multifaceted functions exosomes, small membrane‐bound extracellular vesicles, mediating inflammatory responses cardiac involved this condition. During exosomes are secreted various cells, such as cardiomyocytes, endothelial cells macrophages, which serve critical messengers, transferring proteins, lipids RNA molecules influence recipient thus affecting cellular disease progression. This review summarises basics focuses on exosome‐mediated mechanisms SICM, including their role inflammation, oxidative stress, mitochondrial injury, offering novel insights into exosome‐based therapeutic strategies SICM.

Language: Английский

Citations

0
Affinity Modifications of Porous Microscaffolds Impact Bone Regeneration by Modulating the Delivery Kinetics of Small Extracellular Vesicles DOI

Yike Gao,

Xiaojing Yuan,

Ruoheng Gu

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

Biomaterials functionalized with small extracellular vesicles (sEVs) hold great regenerative potential, and their therapeutic efficacy hinges on the delivery kinetics of sEVs. Achieving rapid stable loading, along precisely controlled release sEVs, necessitates affinity modifications biomaterials. Here, we provide a quantitative description interaction between sEVs various molecules (i.e., polydopamine (PDA), tannic acid (TA), heparin, polyethylenimine (PEI), calcium phosphate (CaP)) through molecular dynamics simulation. The strengths followed order PDA < heparin TA CaP PEI. To tailor stem cells from human exfoliated deciduous teeth (SHED)-derived concentration-dependent bioactivities, employed two representative molecules, namely CaP, to modify PLGA porous microscaffolds (PLGA MS), resulting in PDA-modified MS (PDA@MS) biomineralized (B/PDA@MS). B/PDA@MS exhibited highest loading efficiency (>20 μg/mg microscaffolds) optimized profile over 21 days. Upon injection into 5 mm defect rat cranial bone, sEV-loaded demonstrated level bone regeneration, new volume fraction (BV/TV) mineral density (BMD) reaching 64.0% 604.5 mg/cm3 within 8 weeks, respectively. This work not only presents microscaffold sustained high osteogenic potential but also offers guidance further design translation sEV-functionalized biomaterials broader applications.

Language: Английский

Citations

0
Roles of Exosomal miRNAs in Asthma: Mechanisms and Applications DOI Creative Commons
Xiaoxue Liu, Jiawei Gao,

Liuxin Yang

et al.

Journal of Asthma and Allergy, Journal Year: 2024, Volume and Issue: Volume 17, P. 935 - 947

Published: Sept. 1, 2024

Asthma is a chronic inflammatory disorder of the airways, characterized by complex interplay genetic, environmental, and immunological factors that contribute to its onset progression. Recent advances in researches have illuminated critical role exosomal microRNAs (miRNAs) pathogenesis development asthma. Exosomes are nano-sized extracellular vesicles facilitate intercellular communication transporting variety bioactive molecules, including miRNAs, play crucial regulating gene expression immune responses, which central processes underlying Exosomal miRNAs emerging as key players asthma due their involvement various aspects disease, regulation inflammation, airway hyperresponsiveness, remodeling. Their ability influence behavior target cells tissues makes them valuable both diagnostic biomarkers potential therapeutic targets. This review aims provide comprehensive overview biogenesis exosomes, functional roles asthma, clinical potential. It will explore mechanisms these pathophysiology, discuss utility diagnosing monitoring highlight ongoing research efforts harness

Language: Английский

Citations

3