Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: 217, P. 115482 - 115482
Published: Nov. 29, 2024
Language: Английский
Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(25)
Published: June 12, 2024
Despite the tremendous clinical potential of nucleic acid–based vaccines, their efficacy to induce therapeutic immune response has been limited by lack efficient local gene delivery techniques in human body. In this study, we develop a hydrogel-based organic electronic device (μEPO) for both transdermal acids and vivo microarrayed cell electroporation, which is specifically oriented toward one-step transfection DNAs subcutaneous antigen-presenting cells (APCs) cancer immunotherapy. The μEPO contains an array microneedle-shaped electrodes with pre-encapsulated dry DNAs. Upon pressurized contact skin tissue, are rehydrated, electrically triggered release DNAs, then electroporate nearby cells, can achieve more than 50% epidermal upper dermal layer. As proof-of-concept, technique employed facilitate neoantigen genes activate antigen-specific enhanced immunotherapy based on DNA vaccination strategy. ovalbumin (OVA) vaccine model, show that high-efficiency APCs OVA-DNAs induces robust cellular humoral responses, including antigen presentation generation IFN-γ + cytotoxic T lymphocytes 10-fold dose sparing over existing intramuscular injection (IM) approach, effectively inhibits tumor growth rodent animals.
Language: Английский
Citations
4
Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: 251, P. 114604 - 114604
Published: March 5, 2025
Language: Английский
Citations
0
Molecular Pharmaceutics, Journal Year: 2025, Volume and Issue: unknown
Published: March 26, 2025
To understand requirements for immunization via the oral mucosa, an in vitro model that recapitulates physical barrier of mouth, allows quantification antigen uptake and permeability mounts inflammatory response to adjuvant is needed. The structure 4 models human mucosa was determined by histochemical staining transepithelial electrical resistance (TEER) measurements. A TR146 based air-liquid interface (ALI) most closely mimicked vivo conditions. This confirmed validation studies using dextran caffeine as diffusant molecules. Apparent coefficients (Papp) adenovirus (Ad) adeno-associated virus (AAV) this were 4.3 × 10-13 2.2 10-10 respectively, while 100% total dose H1N1 influenza remained epithelial layer. Sodium glycocholate a hyperosmotic formulation improved amount Ad (p = 0.02) AAV 0.003) entered epithelium, respectively. Significant amounts IL-6 (45.1 pg/mL), GM-CSF (94.7 pg/mL) IFN-γ (4.3 produced infection. Treatment with AS03-like induced production (34.9 TNF-∝ (43 (121.2 (14.1 pg/mL). highlights contribution differentiated cells immune vaccines adjuvants.
Language: Английский
Citations
0
ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown
Published: April 7, 2025
The emergence of immunotherapy as a revolutionary therapeutic modality has fostered confidence and underscored its potent efficacy in tumor therapy. However, enhancing the by precise judicious administration poses significant challenge. In this context, we have developed disulfide-bearing transdermal nanovaccine integrating thiol-reactive agent lipoic acid (LA) into metal-coordinated cyclic dinucleotide nanoassembly, designated LA-Mn-cGAMP (LMC) nanovaccines. Upon topical application to skin with melanoma, dithiolane moiety LA enables thiol-disulfide dynamic exchange skin, hence facilitating penetration both subcutaneous tissues via thiol-mediated uptake (TMU) mechanism. Our findings demonstrate that LMC significantly enhances STING activation, mitigates immunosuppressive microenvironment (TME), retards melanoma progression. Moreover, remodeled TME amplifies immune checkpoint inhibitors. This advancement offers an strategy for existing agonist therapy, potentially improving biosafety immunotherapy.
Language: Английский
Citations
0
Brazilian Journal of Implantology and Health Sciences, Journal Year: 2024, Volume and Issue: 6(6), P. 209 - 224
Published: June 4, 2024
A revisão narrativa "Desafios na Vacinação Global: Obstáculos, Estratégias e Perspectivas Futuras" explora a importância os desafios da vacinação como uma ferramenta essencial saúde pública. tem sido crucial para erradicação de doenças devastadoras promoção imunidade coletiva. No entanto, ascensão dos movimentos antivacinas, disseminação desinformação hesitação vacinal representam obstáculos significativos. destaca evolução das vacinas, desde as primeiras práticas inoculação até tecnologias modernas, vacinas mRNA. Além disso, aborda necessidade estratégias eficazes educação comunicação aumentar confiança pública nas vacinas. cooperação global o desenvolvimento contínuo são essenciais enfrentar infecciosas emergentes garantir um futuro mais saudável seguro. preparação futuras pandemias fundamentais proteger alcançar
Citations
0
Futurity Medicine., Journal Year: 2024, Volume and Issue: unknown, P. 57 - 67
Published: May 17, 2024
Aims: To improve existing knowledge in aetiology, diagnosis, management and treatment of allergic respiratory disorders military personnel. The paper also discussed specific treatments, best practices, key sources advances the physical environment for optimal health improvement. Study design: A comprehensive review literature spanning from 2019 to 2024. Methodology: on minimising conditions wounded servicemen utilised PubMed search relevant 2019-2024. Following screening, six studies were chosen 321 records. Results: findings summarised this presented valuable information allergy general among soldiers. research included works with large samples, one which over 20 million members active veterans. Major risks demonstrated environmental factors; 77% participants experienced a contact dermatitis adhesive tapes, 61% colophonium. Accident highlighted, especially factors such as fine particulate matter dust storms. Measures treating involved both pharmacological non-pharmacological methods individual evaluations tests like pulmonary function chest CT scans. All these figures implied need combating issues an effective way within framework groups. Scientific Novelty: This contributed about personnel developed personalised treatment, prevention early diagnostics scale epidemiological data promote combat readiness servicemen. Conclusion: mainly focused problems that are more common personnel; hence accurate allergens identification complex plans. use technology application approaches vital population management, improvement its richness, readiness. Therefore, it is important Military healthcare systems incorporate sound practices correspondingly.
Language: Английский
Citations
0
Brazilian Journal of Implantology and Health Sciences, Journal Year: 2024, Volume and Issue: 6(6), P. 651 - 666
Published: June 8, 2024
O desenvolvimento das vacinas contra a COVID-19 representou um marco na história da medicina, permitindo uma resposta global à pandemia. Este artigo revisou os princípios gerais do vacinas, sua eficácia, segurança e impacto saúde pública global. As demonstraram ser altamente eficazes redução gravidade doença prevenção de hospitalizações mortes. No entanto, surgiram preocupações com eventos adversos raros, como trombose trombocitopenia após vacinação, que exigem vigilância contínua. Apesar desses desafios, as continuam desempenhar papel crucial contenção
Citations
0
Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: 217, P. 115482 - 115482
Published: Nov. 29, 2024
Language: Английский
Citations
0