Greater host influence and promiscuity: How an invasive seaweed host has advantages over co-occurring natives DOI Creative Commons
Marjan Ghotbi, Guido Bonthond, Mitra Ghotbi

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 2, 2024

Abstract The surface microbiome of seaweed hosts is a multi-domain biofilm regulated by host-microbe and microbe-microbe interactions. extent to which influence these interactions, potentially affect their resilience invasion success, remains unclear. We experimentally tested whether with history exert more over biofilms than native hosts. Biofilm formation on proxy surfaces adjacent one invasive ( Gracilaria vermiculophylla ) two Fucus serratus, vesiculosus co-occurring was monitored compared mature epiphytic the same Only Gracilaria’s were significantly different in community composition control surfaces. ’s also showed highest similarity algae sharing certain bacterial taxa that absent treatments, indicating colonization influenced host. its microbial network variables, suggesting higher ability invader connectivity associations within biofilm. Meanwhile variability prokaryotic experiments, reflected less robust both biofilms. This suggests addition stronger host, it promiscuous towards potential symbionts from environment. Ultimately, through examining line previous research we found host promiscuity may play an important role acclimate environmental condition successfully thrive new ecosystems.

Language: Английский

Absolute abundance unveilsBasidiobolusas a cross-domain bridge indirectly bolstering gut microbiome homeostasis DOI Creative Commons
Mitra Ghotbi, Jason Stajich, Jason Dallas

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 28, 2024

Abstract The host microbiome is integral to metabolism, immune function, and resilience against pathogens. However, reliance on relative abundance (RA) estimate host-associated microbiomes introduces compositional biases, while limited tools for absolute (AA) quantification hinder broader applications. To address these challenges, we developed DspikeIn ( https://github.com/mghotbi/DspikeIn ), an R package paired with a versatile wet-lab methodology AA quantification. Using RA compare core distributions across herpetofauna orders their natural histories revealed starkly distinct results, driven by aggregate effects, including inherited biases in additional multifactorial influences. Focusing two closely related Desmognathus species demonstrated that enhanced resolution differential analyses minimized false discovery rates (FDR) when identifying enriched taxa gut microbiomes. Keystone identified through network associations also differed between data. For example, Lactococcus Cetobacterium were members Anura Caudata, Basidiobolus Mortierella Chelonia Squamata, facilitating adaptation diverse environments, insights undetectable AA-based analysis further removing the subnetwork increased negative interactions, highlighting its role promoting homeostasis cross-domain connectivity. Despite low redundancy, node exhibited high betweenness, efficiency, degree, serving as critical bridge linking disconnected nodes or modules indirectly supporting stability, consistent Burt’s structural hole theory. represents transformative tool research, enabling transition from delivering more accurate, consistent, comparable results studies. Graphical abstract cheatsheet

Language: Английский

Citations

1
Greater host influence and promiscuity: How an invasive seaweed host has advantages over co-occurring natives DOI Creative Commons
Marjan Ghotbi, Guido Bonthond, Mitra Ghotbi

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 2, 2024

Abstract The surface microbiome of seaweed hosts is a multi-domain biofilm regulated by host-microbe and microbe-microbe interactions. extent to which influence these interactions, potentially affect their resilience invasion success, remains unclear. We experimentally tested whether with history exert more over biofilms than native hosts. Biofilm formation on proxy surfaces adjacent one invasive ( Gracilaria vermiculophylla ) two Fucus serratus, vesiculosus co-occurring was monitored compared mature epiphytic the same Only Gracilaria’s were significantly different in community composition control surfaces. ’s also showed highest similarity algae sharing certain bacterial taxa that absent treatments, indicating colonization influenced host. its microbial network variables, suggesting higher ability invader connectivity associations within biofilm. Meanwhile variability prokaryotic experiments, reflected less robust both biofilms. This suggests addition stronger host, it promiscuous towards potential symbionts from environment. Ultimately, through examining line previous research we found host promiscuity may play an important role acclimate environmental condition successfully thrive new ecosystems.

Language: Английский

Citations

0