Effective Decolorization of Poly-γ-Glutamic Acid Fermentation Broth by Integrated Activated Carbon Adsorption and Isoelectric Point Precipitation of Glutamic Acid DOI Creative Commons

X.M. Si,

Ji‐Cheng Li,

Tingbin Zhao

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(23), P. 5769 - 5769

Published: Dec. 6, 2024

Poly-γ-glutamic acid (γ-PGA) is widely used in the field of biomedicine, food, agriculture, and ecological remediation. For biosynthesis γ-PGA, pigments remaining glutamate are two big problems that impede γ-PGA production by fermentation, a trade-off between decolorization rate recovery during purification process was found. The optimized static activated carbon adsorption conditions for treating 2-times diluted cell-free supernatant (i.e., feed solution) as follows: 0.51% 200-mesh, 1000 iodine value, coal-based carbon, pH 6.0, 140 min, 40 °C. Under conditions, reached 94.42%, 94.22%. During process, were adsorbed on surface monolayer, spontaneous, heat-absorbing, entropy-increasing process. Then, flow dynamic experiment 1 BV/h. However, still problem after adsorption. After isoelectric point (IEP) precipitation glutamic acid, can be recovered, residual pigment further removed. Finally, an integrated IEP developed. rates 95.80% 49.02%, respectively. recovered reused next fermentation

Language: Английский

Novel microbial technologies for optimizing maize plant–soil systems: the multifunctional strain Enterobacter sp. ES1 DOI Open Access
Yufeng Xiao,

Meiqi Dong,

Siya Wang

et al.

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 2, 2025

In recent years, a new microbial technology has emerged to optimize maize field productivity and soil health. This is crucial because of the adverse effects pesticide contamination diseases on yields. Enterobacter sp. ES1, multifunctional strain, effectively degraded 93.09% nicosulfuron within 4 days. Optimal degradation occurred at 35 °C pH 6.0, with 4% inoculum 20 mg L-1 nicosulfuron. Strain ES1 could fix nitrogen solubilize phosphorus potassium. It also detoxified potassium produced chrome azurol S indole-3-acetic acid. Moreover, it promoted rapid seed germination, induced resistance northern leaf blight, up-regulated genes ZmOPR2, ZmPR5, ZmHPL, ZmOX10, ZmPAL, ZmPR1, ZmAOS, ZmCTR1. ES1R-gfp, prepared using green fluorescent protein antibiotic domestication methods, functioned as bacterial fertilizer 31.04% sawdust, 32.96% straw, 12.15% biochar, exhibiting rate 99.65%. The novel strain was verified through pot experiment assessing concentration plant indices, thereby providing foundation for optimizing restoration. © 2025 Society Chemical Industry.

Language: Английский

Citations

0
Rhizobacteria mitigate salinity stress in maize by modulating photosynthesis, antioxidant defense, and rhizosphere microbial diversity DOI Creative Commons
Letian Xu,

Caiyun Xin,

Fasih Ullah Haider

et al.

Plant Stress, Journal Year: 2025, Volume and Issue: unknown, P. 100781 - 100781

Published: Feb. 1, 2025

Language: Английский

Citations

0
Biochar and PGPR Influence Rhizosphere Bacteria and Synergistically Regulate Tomato Seedling Biomass Under Greenhouse Conditions DOI
Zhiqiang Yan,

Yuyuan Xing,

Changli Ma

et al.

Journal of soil science and plant nutrition, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Language: Английский

Citations

0
Effects of poly-γ-glutamic acid on soil pore structure, maize grain filling, yield and water use efficiency under mulched drip irrigation DOI
Zhongmin Zhai, Wenjuan Shi, Lu Liu

et al.

Field Crops Research, Journal Year: 2024, Volume and Issue: 317, P. 109528 - 109528

Published: Aug. 7, 2024

Language: Английский

Citations

2
Isolation, identification, and mechanism analysis of plant growth-promoting rhizobacteria in tobacco DOI Creative Commons
Chuandong Jiang,

Fuyu Peng,

Li Zhang

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 20, 2024

Plant growth, crop yield, and pest disease control are enhanced by PGPR (Plant growth promoting rhizobacteria), which beneficial microorganisms found in a close symbiosis with plant roots. Phytohormones secreted, nutrient uptake is improved, soil properties along the microbiological environment regulated these microorganisms, making them significant focus agricultural research. In this study, efficient strain T1 was isolated screened from tobacco inter-root soil, identified confirmed ITS sequencing technology. Tobacco indicators property changes were observed recorded through potting experiments. The activities of key enzymes (e.g., sucrase, catalase, urease) further determined. High-throughput technology utilized to sequence microbial community, combined macro-genomics analysis, effects on diversity metabolic pathways explored. Following application T1, improvements height, leaf length, width plants. Furthermore, physical chemical notably enhanced, including 26.26% increase phosphorus availability. Additionally, such as urease significantly increased, indicating improved health fertility. Comprehensive joint microbiomics macrogenomics analyses revealed substantial rise populations an enhancement pathways, amino acid metabolism, synthesis, production secondary metabolites. These their functions crucial for This study provides valuable references development innovative fertilizers offers programs sustainable modern agriculture.

Language: Английский

Citations

0
Effective Decolorization of Poly-γ-Glutamic Acid Fermentation Broth by Integrated Activated Carbon Adsorption and Isoelectric Point Precipitation of Glutamic Acid DOI Creative Commons

X.M. Si,

Ji‐Cheng Li,

Tingbin Zhao

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(23), P. 5769 - 5769

Published: Dec. 6, 2024

Poly-γ-glutamic acid (γ-PGA) is widely used in the field of biomedicine, food, agriculture, and ecological remediation. For biosynthesis γ-PGA, pigments remaining glutamate are two big problems that impede γ-PGA production by fermentation, a trade-off between decolorization rate recovery during purification process was found. The optimized static activated carbon adsorption conditions for treating 2-times diluted cell-free supernatant (i.e., feed solution) as follows: 0.51% 200-mesh, 1000 iodine value, coal-based carbon, pH 6.0, 140 min, 40 °C. Under conditions, reached 94.42%, 94.22%. During process, were adsorbed on surface monolayer, spontaneous, heat-absorbing, entropy-increasing process. Then, flow dynamic experiment 1 BV/h. However, still problem after adsorption. After isoelectric point (IEP) precipitation glutamic acid, can be recovered, residual pigment further removed. Finally, an integrated IEP developed. rates 95.80% 49.02%, respectively. recovered reused next fermentation

Language: Английский

Citations

0