Clinical outcomes of single blastocyst transfer with machine learning guided noninvasive chromosome screening grading system in infertile patients DOI Creative Commons
Xiaoxi Li,

Yaxin Yao,

Dunmei Zhao

et al.

Reproductive Biology and Endocrinology, Journal Year: 2024, Volume and Issue: 22(1)

Published: May 23, 2024

Abstract Background Prospective observational studies have demonstrated that the machine learning (ML) -guided noninvasive chromosome screening (NICS) grading system, which we called screening-artificial intelligence (NICS-AI) can be used embryo selection. The current prospective interventional clinical study was conducted to investigate whether this NICS-AI system as a powerful tool for Methods Patients who visited our centre between October 2018 and December 2021 were recruited. Grade A B embryos with high probability of euploidy transferred in NICS group. patients control group selected according traditional morphological grading. Finally, 90 161 compared statistically their outcomes. Results In group, pregnancy rate (70.0% vs. 54.0%, p < 0.001), ongoing (58.9% 44.7%, = live birth (56.7% 42.9%, 0.001) significantly higher than those When female ≥ 35 years old, (67.7% 32.1%, (56.5% 25.0%, (54.8% Regardless had previous record early spontaneous abortion or not, (61.0% 46.9%; 57.9% 34.8%; 33.3% 0%) but differences not significant. Conclusions able improve utilisation rate, especially transfer being preferred, followed by embryos. an effective selection future.

Language: Английский

PGT-A: what’s it for, what’s wrong? DOI
Stéphane Viville, Mohamed Aboulghar

Journal of Assisted Reproduction and Genetics, Journal Year: 2025, Volume and Issue: 42(1), P. 63 - 69

Published: Jan. 1, 2025

Language: Английский

Citations

1
Rare autosomal trisomies detected by non-invasive prenatal testing: an overview of current knowledge DOI Open Access
Lore Lannoo,

Khaila van Straaten,

Jeroen Breckpot

et al.

European Journal of Human Genetics, Journal Year: 2022, Volume and Issue: 30(12), P. 1323 - 1330

Published: July 27, 2022

Language: Английский

Citations

29
Metabolic imaging of human embryos is predictive of ploidy status but is not associated with clinical pregnancy outcomes: a pilot trial DOI
Denny Sakkas,

Colwyn Gulliford,

Goli Ardestani

et al.

Human Reproduction, Journal Year: 2024, Volume and Issue: 39(3), P. 516 - 525

Published: Jan. 9, 2024

Abstract STUDY QUESTION Does fluorescence lifetime imaging microscopy (FLIM)-based metabolic assessment of human blastocysts prior to frozen transfer correlate with pregnancy outcomes? SUMMARY ANSWER FLIM failed distinguish consistent patterns in mitochondrial metabolism between leading compared those that did not. WHAT IS KNOWN ALREADY measurements provide quantitative information on NAD(P)H and flavin adenine dinucleotide (FAD+) concentrations. The embryos has long been linked their viability, suggesting the potential utility aid selection. DESIGN, SIZE, DURATION This was a pilot trial enrolling 121 IVF couples who consented have blastocyst measured using non-invasive imaging. After being warmed, 105 couples’ good-quality underwent 6-min scan controlled temperature gas environment. FLIM-assessed were then transferred without any intervention management. PARTICIPANTS/MATERIALS, SETTING, METHODS Eight parameters obtained from each (4 for 4 FAD): short lifetime, intensity, fraction molecule engaged enzyme. redox ratio (intensity NAD(P)H)/(intensity FAD) also calculated. data combined known metadata analyzed quantify ability differentiate resulted De-identified discarded aneuploid (n = 158) correlations ploidy status other factors. Statistical comparisons performed logistic regression receiver operating characteristic (ROC) curves 5-fold cross-validation averaged over 100 repeats random sampling. AUC values used classes. MAIN RESULTS AND THE ROLE OF CHANCE No showed significant differences resulting versus A produced an ROC 0.58. In contrast, robust AUCs when classifying factors such as comparison Day 5 64) 6 41) (AUC 0.78), inner cell mass trophectoderm 105: 0.88) euploid positive 108) 0.82). LIMITATIONS, REASONS FOR CAUTION study protocol not select which embryo cohort included all high quality. limited number participants sites. Increased power performing more sites may provided stronger conclusion regarding merits use clinically. WIDER IMPLICATIONS FINDINGS Blastocyst was, however, highly distinguishable. addition, regions day consistently revealed by FLIM. While detects features blastocysts, this indicates it does serve effective viability detection tool. be because plays alternative role post-implantation. FUNDING/COMPETING INTEREST(S) sponsored Optiva Fertility, Inc. Boston contributed clinical site services. Becker Hickl, GmbH, system loan. T.S. founder held stock Inc., D.S. E.S. had options during study. TRIAL REGISTRATION NUMBER approved WCG Connexus IRB (Study Number 1298156).

Language: Английский

Citations

8
Molecular profiling of human blastocysts reveals primitive endoderm defects among embryos of decreased implantation potential DOI Creative Commons
Jennifer N. Chousal, Robert Morey, Srimeenakshi Srinivasan

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(2), P. 113701 - 113701

Published: Jan. 26, 2024

Human embryo implantation is remarkably inefficient, and failure remains among the greatest obstacles in treating infertility. Gene expression data from human embryos have accumulated rapidly recent years; however, identification of subset genes that determine successful a challenge. We leverage clinical morphologic grading—known for decades to correlate with potential—and transcriptome analyses matched embryonic abembryonic samples identify factors pathways enriched depleted blastocysts good poor morphology. Unexpectedly, we discovered difference was state extraembryonic primitive endoderm (PrE) development, relative deficiencies morphology blastocysts. Our results suggest success most strongly influenced by compartment deficient PrE development common decreased potential. study provides valuable resource those investigating markers mechanisms implantation.

Language: Английский

Citations

7
Clinical outcomes of single blastocyst transfer with machine learning guided noninvasive chromosome screening grading system in infertile patients DOI Creative Commons
Xiaoxi Li,

Yaxin Yao,

Dunmei Zhao

et al.

Reproductive Biology and Endocrinology, Journal Year: 2024, Volume and Issue: 22(1)

Published: May 23, 2024

Abstract Background Prospective observational studies have demonstrated that the machine learning (ML) -guided noninvasive chromosome screening (NICS) grading system, which we called screening-artificial intelligence (NICS-AI) can be used embryo selection. The current prospective interventional clinical study was conducted to investigate whether this NICS-AI system as a powerful tool for Methods Patients who visited our centre between October 2018 and December 2021 were recruited. Grade A B embryos with high probability of euploidy transferred in NICS group. patients control group selected according traditional morphological grading. Finally, 90 161 compared statistically their outcomes. Results In group, pregnancy rate (70.0% vs. 54.0%, p < 0.001), ongoing (58.9% 44.7%, = live birth (56.7% 42.9%, 0.001) significantly higher than those When female ≥ 35 years old, (67.7% 32.1%, (56.5% 25.0%, (54.8% Regardless had previous record early spontaneous abortion or not, (61.0% 46.9%; 57.9% 34.8%; 33.3% 0%) but differences not significant. Conclusions able improve utilisation rate, especially transfer being preferred, followed by embryos. an effective selection future.

Language: Английский

Citations

6