Assessing the prevalence of FRNA bacteriophages and their correlation with SARS-CoV-2 RNA in the wastewater of Mumbai city DOI Creative Commons
Dipen Desai,

Nishith Desai,

Hima Wani

et al.

Journal of Water and Health, Journal Year: 2024, Volume and Issue: 22(7), P. 1180 - 1194

Published: June 18, 2024

ABSTRACT Bacteriophages have been explored as indicators of the presence human viruses in water. F+ coliphages are particular interest due to their abundance wastewater and some groups surviving treatment process. This study assessed prevalence FRNA bacteriophage (FRNAPH) wastewaters Mumbai city its correlation with SARS-CoV-2. Wastewater samples (raw = 63, treated 99) collected from three plants (WWTPs) were for coliphages, FRNAPH Of 63 raw samples, 92% positive FRNAPH, while SARS-CoV-2 was detected 76% samples; GI RNA most prevalent (96.82%) followed by GII (77.77%), GIII (36.50%), GIV (4.76%). A significant observed between occurrence copies also GIII. These observations suggest that can be further wastewaters. Also, coliphage detection may a cost-effective practical approach monitoring virus elimination during treatment.

Language: Английский

Prevalence and association of oral manifestations with disease severity in patients diagnosed with COVID‑19: A cross‑sectional study DOI Creative Commons

Adriana-Teodora Campeanu,

Mihaela Rus,

Sorina Ispas

et al.

Medicine International, Journal Year: 2024, Volume and Issue: 4(3)

Published: April 10, 2024

Systemic disorders may exhibit early signs when conducting an oral examination. Since the onset of COVID‑19 pandemic, several studies have been published detailing direct impact virus on cavity. The present study aimed to determine whether indeed there are any significant disparities in oropharyngeal manifestations between individuals infected with severe acute respiratory syndrome coronavirus 2 and a control group, has ability invade reproduce inside keratinocytes fibroblasts, resulting development ulcerations superficial lesions. provides overview symptoms that occur at stage illness, most commonly affected regions cavity, including tongue, lips, palate oropharynx examined. In retrospective study, 52 patients were recruited April, 2021 October, 2022. addition, who tested negative for as group. was conducted through thorough examination questionnaire provided all participants. results revealed among cohort from group examined (n=52), proportion (mean, 16.15) displayed manifestations. Specifically, 75% described cavity pain, 69% these had changes teeth color or dental caries. summary, relation prevalence generally lower compared apart pain (30.8%), tonsillitis (17.3%), bleeding (34.6%), (36.5%), recurrence (15.4%) abscesses (7.7%). Thus, whole, without fewer

Language: Английский

Citations

1

Jamaican fruit bat (Artibeus jamaicensis) insusceptibility to mucosal inoculation with SARS-CoV-2 Delta variant is not caused by receptor compatibility DOI Creative Commons
Julia R. Port, Jade C. Riopelle, Sarah van Tol

et al.

npj Viruses, Journal Year: 2024, Volume and Issue: 2(1)

Published: July 16, 2024

Abstract The ancestral sarbecovirus giving rise to SARS-CoV-2 is posited have originated in bats. While causes asymptomatic severe respiratory disease humans, little known about the biology, virus tropism, and immunity of SARS-CoV-2-like sarbecoviruses has been shown infect multiple mammalian species, including various rodent non-human primates, Egyptian fruit We show that can utilize Jamaican bat ( Artibeus jamaicensis) ACE2 spike for entry vitro. Therefore, we investigate as a possible vivo model study reservoir responses. find Delta does not efficiently replicate bats vivo. observe infectious viruses lungs only one animal on day 1 post-inoculation no evidence shedding or seroconversion. This possibly due host factors restricting egress after aborted replication. Furthermore, significant immune gene expression changes tract but do intestinal metabolome inoculation. suggests that, despite its broad range, unable all are an appropriate infection.

Language: Английский

Citations

1

SARS-CoV-2 infection in synanthropic rats from Guayaquil city (Ecuador) during COVID-19 pandemic: a proxy to prevent wild reservoirs in the tropics DOI
Solón Alberto Orlando,

Maria Daniela Mera,

Naomi Mora Jaramillo

et al.

Acta Tropica, Journal Year: 2024, Volume and Issue: 259, P. 107371 - 107371

Published: Aug. 27, 2024

Language: Английский

Citations

1

Editor response for version 1 DOI Creative Commons
Girish Beedessee

Published: Feb. 10, 2024

Introduction The coronavirus illness caused by SARS- CoV-2 can cause multiple organ involvement, with varying degrees of severity. Besides, inhalation as a route for transmission, feco-oral has also been proposed. Its transmission to sewage systems is growing public health issue. Objective To detect SARS-CoV-2 RNA in non-respiratory samples (saliva, urine, and stool) collected from COVID-19 cases, Bihar. Materials methods This Cross-Sectional observational study was conducted January 2021 March 2022 on human samples. A total 345 including saliva (116), stool (97), urine (132) were 143 covid-19 cases. Samples analyzed multiplex RT-PCR targeted against E, ORF 1ab, RdRp gene. Results In this study, out 107(74.8%) positive at least one the Conclusion There high prevalence virus

Language: Английский

Citations

0

Reviewer response for version 1 DOI Creative Commons
Abhishek Padhi

Published: Jan. 30, 2024

Introduction The coronavirus illness caused by SARS- CoV-2 can cause multiple organ involvement, with varying degrees of severity. Besides, inhalation as a route for transmission, feco-oral has also been proposed. Its transmission to sewage systems is growing public health issue. Objective To detect SARS-CoV-2 RNA in non-respiratory samples (saliva, urine, and stool) collected from COVID-19 cases, Bihar. Materials methods This Cross-Sectional observational study was conducted January 2021 March 2022 on human samples. A total 345 including saliva (116), stool (97), urine (132) were 143 covid-19 cases. Samples analyzed multiplex RT-PCR targeted against E, ORF 1ab, RdRp gene. Results In this study, out 107(74.8%) positive at least one the Conclusion There high prevalence virus

Language: Английский

Citations

0

Reviewer response for version 1 DOI Creative Commons
Sweta Singh

Published: Feb. 9, 2024

Introduction The coronavirus illness caused by SARS- CoV-2 can cause multiple organ involvement, with varying degrees of severity. Besides, inhalation as a route for transmission, feco-oral has also been proposed. Its transmission to sewage systems is growing public health issue. Objective To detect SARS-CoV-2 RNA in non-respiratory samples (saliva, urine, and stool) collected from COVID-19 cases, Bihar. Materials methods This Cross-Sectional observational study was conducted January 2021 March 2022 on human samples. A total 345 including saliva (116), stool (97), urine (132) were 143 covid-19 cases. Samples analyzed multiplex RT-PCR targeted against E, ORF 1ab, RdRp gene. Results In this study, out 107(74.8%) positive at least one the Conclusion There high prevalence virus

Language: Английский

Citations

0

Poliomyelitis and COVID-19: a repeated history DOI Creative Commons
Anda Băicuş

Journal of Water and Health, Journal Year: 2024, Volume and Issue: 22(4), P. 717 - 720

Published: Feb. 22, 2024

The measurement of the enterovirus and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in sewage water is relevant early detection introduction or disappearance these viruses ecosystem. We evaluated co-circulation enteroviruses SARS-CoV-2 81 samples collected between September 2021 April 2023 from different regions north southeast Romania, at border with Ukraine. used, for molecular pathogens, multiplex real-time polymerase chain reaction (PCR) assay produced Respiratory 2.1 Plus panel Biofire Film array. isolation was performed on cell culture lines, accordance World Health Organization (WHO) recommendations. By investigations, we detected 22 (27%) samples, human rhinovirus/enterovirus 64 (79%) samples. 27 (33,33%) were positive non-polio enteroviruses, no poliovirus strains isolated, proving maintenance polio-free status Romania. In an emergency situation, pathogens using a PCR system integrating sample preparation, amplification, detection, analysis 1 h could be implemented.

Language: Английский

Citations

0

Coronavirus Disease 2019 Histology and Histopathology DOI
Aniello Maiese, A Manetti, Vittorio Fineschi

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 740 - 750

Published: March 8, 2024

Language: Английский

Citations

0

Title of Manuscript: Prevalence of SARS- CoV-2 virus in saliva, stool, and urine samples of COVID-19 patients in Bihar, India DOI Creative Commons
Nupur Meghna, Archana Archana, Divendu Bhushan

et al.

Published: March 12, 2024

Introduction The coronavirus illness caused by SARS- CoV-2 can cause multiple organ involvement, with varying degrees of severity. Besides, inhalation as a route for transmission, feco-oral has also been proposed. Its transmission to sewage systems is growing public health issue. Objective To detect SARS-CoV-2 RNA in non-respiratory samples (saliva, urine, and stool) collected from COVID-19 cases, Bihar. Materials methods This Cross-Sectional observational study was conducted January 2021 March 2022 on human samples. A total 345 including saliva (116), stool (97), urine (132) were 143 covid-19 cases. Samples analyzed multiplex RT-PCR targeted against E, ORF 1ab, RdRp gene. Results In this study, out 107(74.8%) positive at least one the Conclusion There high prevalence virus

Language: Английский

Citations

0

Editor response for version 2 DOI Creative Commons
Girish Beedessee

Published: March 18, 2024

Introduction: The coronavirus illness caused by SARS- CoV-2 can cause multiple organ involvement, with varying degrees of severity. Besides, inhalation as a route for transmission, feco-oral has also been proposed. Its transmission to sewage systems is growing public health issue. Objective: To detect SARS-CoV-2 RNA in non-respiratory samples (saliva, urine, and stool) collected from COVID-19 cases, Bihar. Materials methods: This Cross-Sectional observational study was conducted January 2021 March 2022 on human samples. A total 345 including saliva (116), stool (97), urine (132) were 143 covid-19 cases. Samples analyzed multiplex RT-PCR targeted against E, ORF 1ab, RdRp gene. Results: In this study, out 107(74.8%) positive at least one the Conclusion: There high prevalence virus

Language: Английский

Citations

0