The Female Vaginal Microbiome in Health and Bacterial Vaginosis

Frontiers in Cellular and Infection Microbiology, Journal Year: 2021, Volume and Issue: 11

Published: April 7, 2021

The vaginal microbiome is an intricate and dynamic microecosystem that constantly undergoes fluctuations during the female menstrual cycle woman’s entire life. A healthy dominated by Lactobacillus which produce various antimicrobial compounds. Bacterial vaginosis (BV) characterized loss or sharp decline in total number of a corresponding marked increase concentration anaerobic microbes. BV highly prevalent disorder microbiota among women reproductive age globally. confirmed to be associated with adverse gynecologic obstetric outcomes, such as sexually transmitted infections, pelvic inflammatory disease, preterm birth. Gardnerella vaginalis most common microorganism identified from BV. It predominant microbe polymicrobial biofilms could shelter G. other BV-associated microbes host environments. Many efforts have been made our understanding health Thus, improved novel accurate diagnosis therapeutic strategies for developed. This review covers features microbiome, BV, diseases, treatment emphasis on recent research progresses.

Language: Английский

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Endometrial microbiota composition is associated with reproductive outcome in infertile patients

Microbiome, Journal Year: 2022, Volume and Issue: 10(1)

Published: Jan. 4, 2022

Abstract Background Previous evidence indicates associations between the female reproductive tract microbiome composition and outcome in infertile patients undergoing assisted reproduction. We aimed to determine whether endometrial microbiota is associated with outcomes of live birth, biochemical pregnancy, clinical miscarriage or no pregnancy. Methods Here, we present a multicentre prospective observational study using 16S rRNA gene sequencing analyse fluid biopsy samples before embryo transfer cohort 342 asymptomatic for infection treatments. Results A dysbiotic profile composed Atopobium , Bifidobacterium Chryseobacterium Gardnerella Haemophilus Klebsiella Neisseria Staphylococcus Streptococcus was unsuccessful outcomes. In contrast, Lactobacillus consistently enriched birth Conclusions Our findings indicate that useful biomarker predict outcome, offering an opportunity further improve diagnosis treatment strategies.

Language: Английский

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The Endometrial Microbiome and Its Impact on Human Conception

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(1), P. 485 - 485

Published: Jan. 1, 2022

Changes in the female genital tract microbiome are consistently correlated to gynecological and obstetrical pathologies, dysbiosis can impact reproductive outcomes during fertility treatment. Nonetheless, a consensus regarding physiological core inside uterine cavity has not been reached due myriad of study limitations, such as sample size experimental design variations, influence endometrial bacterial communities on human reproduction remains debated. Understanding healthy microbiota how changes its composition affect would potentially allow personalized treatment through management assisted therapies, ultimately leading improvement clinical outcomes. Here, we review current knowledge it relates conception.

Language: Английский

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The Vaginal Microbiome in Health and Disease—What Role Do Common Intimate Hygiene Practices Play?

Microorganisms, Journal Year: 2023, Volume and Issue: 11(2), P. 298 - 298

Published: Jan. 23, 2023

The vaginal microbiome is a dynamic, sensitive microenvironment. hallmark of ‘healthy’ currently believed to be one dominated by Lactobacillus spp., which acidifies the environment and help protect against invading pathogens. However, ‘normal’ often difficult, if not impossible, characterise given that it varies in response numerous variables, including pregnancy, menstrual cycle, contraceptive use, diet, ethnicity, stress. A Lactobacillus-depleted has been linked variety adverse health outcomes, preterm birth (PTB), bacterial vaginosis (BV), increased risk sexually transmitted infections. latter two these have also associated with feminine intimate hygiene practices, many are practised without any evidence benefits. most extensively studied practice douching, known cause dysbiosis, predisposing women BV, pelvic inflammatory disease, PTB. little impact practices products on microbiome. This review aims outline major factors influencing common infections, as well summarise current research surrounding

Language: Английский

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Clinical Relevance of Vaginal and Endometrial Microbiome Investigation in Women with Repeated Implantation Failure and Recurrent Pregnancy Loss

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(1), P. 622 - 622

Published: Jan. 3, 2024

Recent studies have investigated if and how the vaginal endometrial microbiome might affect receptivity reproductive health. Although there is no consensus on existence of a core uterine yet, evidence shows that dominance Lactobacillus spp. in female tract generally associated with eubiosis improved chances successful implantation an ongoing pregnancy. Conversely, dysbiosis can cause local inflammation increase pro-inflammatory cytokines, compromising integrity mucosa potentially hampering embryonic implantation. This review provides critical appraisal influence as parts fertility outcomes, focusing repeated failure (RIF) recurrent pregnancy loss (RPL). It seems RIF well RPL are both diversity lower system.

Language: Английский

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Antispasmodic activity of carnosic acid extracted from rosmarinus officinalis: Isolation, spectroscopic characterization, DFT studies, and in silico molecular docking investigations

Journal of Molecular Structure, Journal Year: 2022, Volume and Issue: 1260, P. 132795 - 132795

Published: March 9, 2022

Language: Английский

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Impact of Lactobacillus crispatus-containing oral and vaginal probiotics on vaginal health: a randomised double-blind placebo controlled clinical trial

Beneficial Microbes, Journal Year: 2023, Volume and Issue: 14(2), P. 143 - 152

Published: March 1, 2023

Health of reproductive tract is tightly associated with balance microbial communities in this area. Bacterial vaginosis (BV) and vulvovaginal candidiasis (VVC) represent common disturbances vaginal communities. Vaginal discharge due to BV or VVC a very frequent reason for visiting gynaecologist. We aimed evaluate the impact novel evidence-based probiotics on patients. The study group included 89 93 patients (aged 18-50 years) who were recruited into randomised double-blind placebo-controlled two-arm parallel trial. each diagnosis received oral probiotic capsules, placebo capsules during 3 months. A capsule contained two (DSM32717 DSM32720, case BV) three (DSM32720, DSM32718 DSM32716, VVC) Lactobacillus crispatus strains. Vaginal, intestinal general health was monitored weekly by questionnaire. Blood analyses done beginning at end samples collected monthly, microscopic molecular performed. revealed that both reduced signs symptoms Remarkable improvement noted Nugent score, amount smell discharge, but also itching/irritation. Consumption significantly increased lactobacilli counts their vagina while mean proportion some BV-related bacteria decreased. In patients, lowered combined score most important symptoms, conclusion, formulations well-focused L. strains are effective against being suitable administration. Clinical trial registration: ISRCTN34840624, BioMed Central

Language: Английский

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The Impact of the Female Genital Microbiota on the Outcome of Assisted Reproduction Treatments

Microorganisms, Journal Year: 2023, Volume and Issue: 11(6), P. 1443 - 1443

Published: May 30, 2023

The vaginal microbiota plays a critical role in the health of female genital tract, and its composition contributes to gynecological disorders infertility. Lactobacilli are dominant species tract: their production lactic acid, hydrogen peroxide, bacteriocins prevents invasion growth pathogenic microorganisms. Several factors such as hormonal changes, age reproduction, sexual practices, menstrual cycle, pregnancy, antimicrobial drugs use can cause imbalance dysbiosis microbiota. This review aims highlight impact Assisted Reproductive Technology techniques (ART) it examines that influence microbiota, consequences dysbiosis, potential interventions restore healthy tract.

Language: Английский

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Gardnerella Revisited: Species Heterogeneity, Virulence Factors, Mucosal Immune Responses, and Contributions to Bacterial Vaginosis

Infection and Immunity, Journal Year: 2023, Volume and Issue: 91(5)

Published: April 18, 2023

species are associated with bacterial vaginosis (BV) and have been investigated as etiological agents of the condition. Nonetheless, isolation this taxon from healthy individuals has raised important questions regarding its role. Recently, using advanced molecular approaches,

Language: Английский

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Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment

Cell, Journal Year: 2024, Volume and Issue: 187(19), P. 5413 - 5430.e29

Published: Aug. 19, 2024

Bacterial vaginosis (BV), a common syndrome characterized by Lactobacillus-deficient vaginal microbiota, is associated with adverse health outcomes. BV often recurs after standard antibiotic therapy in part because antibiotics promote microbiota dominance Lactobacillus iners instead of crispatus, which has more beneficial associations. Strategies to L. crispatus and inhibit are thus needed. We show that oleic acid (OA) similar long-chain fatty acids simultaneously enhance growth. These phenotypes require OA-inducible genes conserved related lactobacilli, including an oleate hydratase (ohyA) putative efflux pump (farE). FarE mediates OA resistance, while OhyA robustly active the enhances bacterial fitness biochemically sequestering derivative form only ohyA-harboring organisms can exploit. promotes effectively than vitro model, suggesting metabolite-based treatment approach.

Language: Английский

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