bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 7, 2024
Abstract
Microalgae
are
well-known
for
their
role
as
sustainable
bio-factories,
offering
a
promising
solution
to
the
global
food
and
nutrition
crisis.
To
clarify
potential
of
Chlorella
sorokiniana
UTEX
1230
applications,
particularly
an
alternative
protein
source,
study
employed
mixotrophic
cultivation
mode
with
sodium
acetate
(NaAc)
cost-effective
organic
carbon
(NaAc-C)
source.
Varying
levels
NaAc-C
nitrate-sourced
nitrogen
were
investigated,
optimizing
effect
metabolic
characteristics
microalgal
growth.
The
designed
heterotrophic
confirmed
ability
C.
grow
on
NaAc-C,
then
cultures,
when
supported
by
both
CO
2
,
exhibited
superior
growth
performance,
achieving
double
biomass
concentration
compared
autotrophic
control.
addition
(750
mg/L
NaNO₃)
facilitated
thorough
metabolism
enhanced
photosynthetic
activity
indicated
196%
increase
in
pigment
levels,
which
resulted
maximum
2.82
g/L
150
mM
group.
A
detailed
analysis
concentrations
over
time
revealed
that
higher
availability
led
greater
accumulation
was
degraded
support
essential
life
activities
under
starvation.
Therefore,
it
is
suggested
supplementing
nitrate
3
rd
day
harvesting
4
th
could
be
strategically
implemented
yield
from
0.17
g/L/d
0.34
g/L/d.
These
findings
offer
theoretical
guidance
further
refining
this
strain
use
protein.
Bioresource Technology,
Journal Year:
2024,
Volume and Issue:
406, P. 131022 - 131022
Published: June 22, 2024
Membrane
bioreactors
(MBRs)
hold
significant
promise
for
wastewater
treatment,
yet
the
persistent
challenge
of
membrane
fouling
impedes
their
practical
application.
One
promising
solution
lies
in
synergy
between
microalgae
and
bacteria,
offering
efficient
nutrient
removal,
reduced
energy
consumption,
potential
mitigation
extracellular
polymeric
substances
(EPS)
concentrations.
Inoculating
presents
a
avenue
to
address
MBRs.
This
review
marks
first
exploration
utilizing
control
MBR
systems.
The
begins
with
comprehensive
overview
evolution
distinctive
traits
microalgae-MBRs.
It
goes
further
insight
into
performance
underlying
mechanisms
facilitating
reduction
through
intervention.
Moreover,
not
only
identifies
challenges
inherent
employing
MBRs
but
also
illuminates
prospective
pathways
future
advancement
this
burgeoning
field.
Physics of Fluids,
Journal Year:
2024,
Volume and Issue:
36(4)
Published: April 1, 2024
Marine
microalgae
are
widely
present
in
the
natural
environment,
exhibiting
a
significant
economic
value.
However,
during
inoculation
and
cultivation
process
of
microalgae,
introduction
unwanted
algae
is
bound
to
trigger
nutrient
competition,
leading
decrease
growth
rate
consequently
impacting
their
value
production.
To
address
this
issue,
study
integrates
optically
induced
dielectrophoresis
(ODEP)
manipulation
technology
based
on
continuous
flow
microfluidic
system.
A
two-stage
cell
filter,
utilizing
two
virtual
optical
spots,
designed
manufactured.
Leveraging
size
differences
between
separation
purification
mixed
samples
containing
Chlorella
vulgaris
Haematococcus
pluvialis
achieved
within
microchannels.
Additionally,
optimal
ODEP
conditions
for
algal
liquid
samples,
comprising
C.
H.
pluvialis,
demonstrated,
including
appropriate
alternating
current
voltage
(6
V),
frequency
(100
kHz),
light
spot
width
(40
μm),
sample
(0.9
μl/min).
Analysis
collected
at
chip's
outlet
reveals
reduction
proportion
from
37.5%
1.2%
after
separation.
In
summary,
proposes
an
system
capable
continuously
separating
purifying
with
different
biological
characteristics,
showcasing
its
potential
as
alternative
traditional
labor-intensive
techniques.
Journal of Agriculture and Food Research,
Journal Year:
2024,
Volume and Issue:
18, P. 101387 - 101387
Published: Sept. 3, 2024
Microalgae,
known
for
their
land-sparing
cultivation
and
remarkable
photosynthetic
efficiency,
play
a
crucial
role
in
advancing
sustainable
development
goals.
This
study
explored
the
use
of
cost-effective
inorganic
carbon
(the
ionic
form
CO2)
organic
sources
(lactose,
primary
sugar
whey
wastewater)
to
support
growth
Chlorella
Sorokiniana
UTEX
1230,
improved
protein
content
through
nitrogen
supply
optimization.
NaHCO3
proved
be
more
effective
than
Na2CO3,
resulting
3.4-fold
increase
biomass
concentration,
with
an
initial
5
mM
HCO3¯
concentration
compared
basal
medium.
Further
addition
0.75%
lactose
led
4.05-fold
maximum
specific
rate
at
1.28/d,
695.88
mg
biomass/L,
productivity
250.91
mg/L/d.
Nitrogen
supplementation
greatly
increased
from
16.41%
(250
mg/L
NaNO3)
52.95%
(1000
NaNO3).
These
results
potential
utilizing
wastewater
bioremediation
produce
high-value
via
microalgal
cultivation.
