Optimizing algal hydrogen photoproduction: a simplified and efficient protocol for anoxic induction in a semi‐autotrophic approach DOI Creative Commons
Fatemeh Khosravitabar,

Kashif M. Shaikh,

Henry L. S. Cheung

et al.

Physiologia Plantarum, Journal Year: 2025, Volume and Issue: 177(2)

Published: March 1, 2025

Abstract Green microalgae, such as Chlamydomonas reinhardtii , show great potential for producing green hydrogen using only water and sunlight, with no carbon emissions. However, sustainable production requires addressing the hydrogenase sensitivity to oxygen enhancing electron allocation this enzyme. Previous methods photoproduction rely on a brief nitrogen flushing followed by dark incubation phase establish anoxia prior exposure high light. In study, we present straightforward protocol involving mixotrophic growth semi‐autotrophic phase. During phase, extended induced in liquid algae culture, even though was still headspace. Anoxia maintained under light at moderate intensity (120 μmol m −2 s −1 ) controlled temperature of 30°C, an efficient mixing system. Throughout monitored dissolved levels culture alongside traditional measurements Using our pgr5 mutant C. achieved maximum specific rate 72 H₂ mg Chl h average 30–35 over 10 hours illumination. Additional steps anoxia, resulting total yield 220 ± 20 mL L 48 This performance is attributed maintaining redox balance plastoquinone pool minimizing photodamage photosystem II complex. Our offers significant advancement scalable production.

Language: Английский

Optimizing algal hydrogen photoproduction: a simplified and efficient protocol for anoxic induction in a semi‐autotrophic approach DOI Creative Commons
Fatemeh Khosravitabar,

Kashif M. Shaikh,

Henry L. S. Cheung

et al.

Physiologia Plantarum, Journal Year: 2025, Volume and Issue: 177(2)

Published: March 1, 2025

Abstract Green microalgae, such as Chlamydomonas reinhardtii , show great potential for producing green hydrogen using only water and sunlight, with no carbon emissions. However, sustainable production requires addressing the hydrogenase sensitivity to oxygen enhancing electron allocation this enzyme. Previous methods photoproduction rely on a brief nitrogen flushing followed by dark incubation phase establish anoxia prior exposure high light. In study, we present straightforward protocol involving mixotrophic growth semi‐autotrophic phase. During phase, extended induced in liquid algae culture, even though was still headspace. Anoxia maintained under light at moderate intensity (120 μmol m −2 s −1 ) controlled temperature of 30°C, an efficient mixing system. Throughout monitored dissolved levels culture alongside traditional measurements Using our pgr5 mutant C. achieved maximum specific rate 72 H₂ mg Chl h average 30–35 over 10 hours illumination. Additional steps anoxia, resulting total yield 220 ± 20 mL L 48 This performance is attributed maintaining redox balance plastoquinone pool minimizing photodamage photosystem II complex. Our offers significant advancement scalable production.

Language: Английский

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