Changes in the Morphology and Antioxidant Status of European Red Deer Sperm Stored in the Epididymides and in a Liquid State DOI Creative Commons
Nicoletta M. Neuman, Aleksandra Orzołek, Żaneta Steiner-Bogdaszewska

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(11), P. 1653 - 1653

Published: May 31, 2024

The aim of this study was to evaluate the motility, morphology, and antioxidant status European red deer sperm stored in a liquid state (variant I) epididymides II). Spermatozoa were harvested post-mortem from cauda epididymis. Sperm samples both variants for up six days (D6) at 5 °C. assessed viability, activity enzymes (superoxide dismutase, SOD; glutathione peroxidase, GPx; catalase, CAT), lipid peroxidation (malondialdehyde, MDA, content). analyzed on storage 0, 2, 4, 6 (D0-D6). Storage time method significantly (

Language: Английский

The impact of environmental and boar breed factors on the quality of semen used for artificial insemination in Tanzania DOI

Violet Cherotich Kemoi,

Isaac P. Kashoma, M. K. Matiko

et al.

Applied Veterinary Research, Journal Year: 2025, Volume and Issue: 3(3), P. 2024012 - 2024012

Published: Feb. 16, 2025

Semen production among commercial boar centers is influenced by various factors, including environmental temperature, humidity, breed, age, nutrition, and frequency of collection. This study aimed to investigate the effects humidity on quality semen used for artificial insemination (AI) in Tanzania. Ninety ejaculates were collected from six boars, with two boars representing each Duroc, Large White, Dupi breeds. samples via gloved hand technique, individual temperatures measured a digital infrared thermometer. The temperature recorded meteorological devices. assessed volume, sperm concentration, progressive motility, viability, morphology, acrosomal integrity. data was stored Microsoft Excel analyzed SPSS software. mean standard deviation pH, integrity per ejaculate 167.78 ± 67.31 ml, 7.27 0.48, 2.54x10⁸ 9.05×10⁶ sperm/mL, 91.58 5.60%, 86.91 5.85%, 93.84± 2.34%, 90.04 4.76%, respectively. Temperature negatively impacted acrosome White no Duroc positive relationship boars. associated viability concluded that are more tolerant suggesting need breed-specific management strategies increase tropical swine industry.

Language: Английский

Citations

0

Alteration in sperm mitochondrial membrane potential and antioxidant biomarkers in summer adversely affects Hampshire-Ghungroo crossbred boar semen fertility in sub-tropical climate DOI Creative Commons
Mahak Singh,

Apanai Celina,

Rahul Katiyar

et al.

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: April 17, 2025

In temperate regions, boars experience environmental heat stress due to the subtropical climate, leading their semen quality and fertility being severely compromised compared indigenous breeds. Considering above effect, present study aimed evaluate effect of season on quality, seminal plasma antioxidant status, in vivo crossbred exotic (50%) inheritance a climate. A total 14 Hampshire-Ghungroo were used for this investigation, which took place both summer winter. Sperm characteristics, namely motility, viability, abnormality, acrosomal integrity, hypo-osmotic swelling test (HOST) results, mitochondrial membrane potential (MMP) evaluated. kinematics parameters assessed using computer-assisted analysis (CASA). Antioxidant biomarkers (glutathione peroxidase, GPx; catalase, CAT; capacity, TAC) lipid peroxidation (malondialdehyde, MDA) analyzed boars' plasma. The had significant (p < 0.01) negative impact reaction time false mounts, whereas volume sperm concentration significantly higher winter season. Similarly, abnormalities lower winter, parameters, progressive HOST reactivity, improved. However, during summer, MMP was fresh samples after 72 h storage. Season 0.05) following parameters: average path velocity, straight-line curve linear amplitude lateral head displacement, beat cross frequency. Semen characteristics improved cold storage with those summer. (TAC, MDA, CAT, GPx). Furthermore, farrowing rate conclusion, our results showed that low boar downregulation lead poor To alleviate heat-stress-induced optimize subtropics, there is need scientific interventions terms genetics [less (below 50%)], nutrition, management.

Language: Английский

Citations

0

Predicting Boar Sperm Survival during Liquid Storage Using Vibrational Spectroscopic Techniques DOI Creative Commons
Serge Leugoué Kameni, Bryan Semon, Li-Dunn Chen

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(10), P. 763 - 763

Published: Sept. 26, 2024

Artificial insemination (AI) plays a critical role in livestock reproduction, with semen quality being essential. In swine, AI primarily uses cool-stored adhering to industry standards assessed through routine analysis, yet fertility inconsistencies highlight the need for enhanced evaluation. Over 10-day storage at 17 °C, boar samples were analyzed motility, morphology, sperm membrane integrity, apoptosis, and oxidative stress indicators. Additionally, machine learning tools employed explore potential of Raman near-infrared (NIR) spectroscopy enhancing sample Sperm motility morphology gradually decreased during storage, distinct groups categorized as “Good” or “Poor” survival according on Day 7 storage. Initially similar 0 collection, revealed significantly lower total (21.69 ± 4.64% vs. 80.19 1.42%), progressive (4.74 1.71% 39.73 2.57%), normal (66.43 2.60% 87.91 1.92%) than their counterparts by 7, using computer-assisted analyzer. Furthermore, had higher levels apoptotic cells, damage, intracellular reactive oxygen species 0. Conversely, maintained antioxidant capacity. outperformed NIR, providing distinctive spectral profiles aligned biochemical changes enabling prediction Overall, coupled might assist evaluation prognosis.

Language: Английский

Citations

1

Changes in the Morphology and Antioxidant Status of European Red Deer Sperm Stored in the Epididymides and in a Liquid State DOI Creative Commons
Nicoletta M. Neuman, Aleksandra Orzołek, Żaneta Steiner-Bogdaszewska

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(11), P. 1653 - 1653

Published: May 31, 2024

The aim of this study was to evaluate the motility, morphology, and antioxidant status European red deer sperm stored in a liquid state (variant I) epididymides II). Spermatozoa were harvested post-mortem from cauda epididymis. Sperm samples both variants for up six days (D6) at 5 °C. assessed viability, activity enzymes (superoxide dismutase, SOD; glutathione peroxidase, GPx; catalase, CAT), lipid peroxidation (malondialdehyde, MDA, content). analyzed on storage 0, 2, 4, 6 (D0-D6). Storage time method significantly (

Language: Английский

Citations

0