Polyesterase activity is widespread in the family IV carboxylesterases from bacteria DOI Creative Commons

Hairong Ma,

Anna N. Khusnutdinova,

Sofia Lemak

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 481, P. 136540 - 136540

Published: Nov. 16, 2024

Language: Английский

State-of-the-art advances in biotechnology for polyethylene terephthalate bio-depolymerization DOI Creative Commons
Yajun Liu, Jie Zhou, Yanwei Li

et al.

Green Carbon, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Discovery, design, and engineering of enzymes based on molecular retrobiosynthesis DOI Creative Commons

Ancheng Chen,

Xiangda Peng, Tao Shen

et al.

mLife, Journal Year: 2025, Volume and Issue: 4(2), P. 107 - 125

Published: March 28, 2025

Abstract Biosynthesis—a process utilizing biological systems to synthesize chemical compounds—has emerged as a revolutionary solution 21st‐century challenges due its environmental sustainability, scalability, and high stereoselectivity regioselectivity. Recent advancements in artificial intelligence (AI) are accelerating biosynthesis by enabling intelligent design, construction, optimization of enzymatic reactions systems. We first introduce the molecular retrosynthesis route planning biochemical pathway including single‐step algorithms AI‐based design tools. highlight advantages large language models addressing sparsity data. Furthermore, we review enzyme discovery methods based on sequence structure alignment techniques. Breakthroughs structural prediction expected significantly improve accuracy discovery. also summarize for de novo generation nonnatural or orphan reactions, focusing functional annotation techniques reaction small molecule similarity. Turning engineering, discuss strategies thermostability, solubility, activity, well applications AI these fields. The shift from traditional experiment‐driven data‐driven computationally driven is already underway. Finally, present potential provide perspective future research directions. envision expanded biocatalysis drug development, green chemistry, complex synthesis.

Language: Английский

Citations

0
Recycling processes of polyester-containing textile waste–A review DOI Creative Commons
Jana Elisabeth-Anna Enking, Amrei Becker,

Gilbert Schu

et al.

Resources Conservation and Recycling, Journal Year: 2025, Volume and Issue: 219, P. 108256 - 108256

Published: March 31, 2025

Language: Английский

Citations

0
Chemical Depolymerization of Polyethylene Terephthalate and Its Blends: Enhanced Strategies for Efficient Circularity DOI
Shun Zhang, Xuan Zhao, Xuehui Liu

et al.

Progress in Polymer Science, Journal Year: 2025, Volume and Issue: unknown, P. 101958 - 101958

Published: April 1, 2025

Language: Английский

Citations

0
Exploring the pH dependence of an improved PETase DOI
Cyril Charlier,

Sabine Gavalda,

Jelena Grga

et al.

Biophysical Journal, Journal Year: 2024, Volume and Issue: 123(12), P. 1542 - 1552

Published: April 25, 2024

Language: Английский

Citations

3
Selective Modification of the Product Profile of Biocatalytic Hydrolyzed PET via Product‐specific Medium Engineering DOI Creative Commons
Tobias Heinks,

Katrin Hofmann,

Simon Last

et al.

ChemSusChem, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 6, 2024

Over the past years, enzymatic depolymerization of PET, one most widely used plastics worldwide, has become very efficient leading to end products terephthalic acid (TPA) and ethylene glycol (EG) for PET re-synthesis. Potent alternatives these monomers are intermediates BHET MHET, mono- di-esters TPA EG which avoid total hydrolysis can serve as single starting materials direct re-polymerization. This study therefore aimed selectively prepare those through reaction medium engineering during biocatalytic PET. After a comparative pre-screening 12 PET-hydrolyzing enzymes, two them (LCC

Language: Английский

Citations

3
Identification and expression of MarCE, a marine carboxylesterase with synthetic ester‐degrading activity DOI Creative Commons
Clodagh M. Carr,

Frederike Göttsch,

Bruno Francesco Rodrigues de Oliveira

et al.

Microbial Biotechnology, Journal Year: 2024, Volume and Issue: 17(6)

Published: June 1, 2024

Carboxylic ester hydrolases with the capacity to degrade polyesters are currently highly sought after for their potential use in biological degradation of PET and other chemically synthesized polymers. Here, we describe MarCE, a carboxylesterase family protein identified via genome mining Maribacter sp. isolate from marine sponge Stelligera stuposa. Based on phylogenetic analysis, MarCE its closest relatives belong marine-associated genera Cytophaga-Flavobacterium-Bacteroides taxonomic group appear evolutionarily distinct any homologous carboxylesterases that have been studied date terms structure or function. Molecular docking revealed putative binding BHET, short-chain derivative, onto predicted three-dimensional structure. The synthetic ester-degrading activity was subsequently confirmed by MarCE-mediated hydrolysis 2 mM BHET substrate, indicated release breakdown products MHET TPA, which were measured, respectively, as 1.28 0.12 following 2-h incubation at 30°C. findings this study provide further insight into carboxylic hydrolases, display unique functional plasticity resulting adaptation complex fluctuating environmentsw.

Language: Английский

Citations

2
Magnetic silica-coated cutinase immobilized via ELPs biomimetic mineralization for efficient nano-PET degradation DOI
Guanzhang Liu, Hang Yuan,

Yaxin Chen

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 279, P. 135414 - 135414

Published: Sept. 6, 2024

Language: Английский

Citations

2
GRACE: Generative Redesign in Artificial Computational Enzymology DOI Creative Commons
Ruei-En Hu, Chi‐Hua Yu, I‐Son Ng

et al.

ACS Synthetic Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 8, 2024

Designing

Language: Английский

Citations

2
Two-step biocatalytic conversion of post-consumer polyethylene terephthalate into value-added products facilitated by genetic and bioprocess engineering DOI Creative Commons

Gina Welsing,

Birger Wolter,

Greta E K Kleinert

et al.

Bioresource Technology, Journal Year: 2024, Volume and Issue: unknown, P. 131837 - 131837

Published: Nov. 1, 2024

Language: Английский

Citations

2