Vascularized platforms for investigating cell communication via extracellular vesicles DOI
Junyoung Kim, Jooyoung Ro, Yoon‐Kyoung Cho

et al.

Biomicrofluidics, Journal Year: 2024, Volume and Issue: 18(5)

Published: Sept. 1, 2024

The vascular network plays an essential role in the maintenance of all organs body via regulated delivery oxygen and nutrients, as well tissue communication transfer various biological signaling molecules. It also serves a route for drug administration affects pharmacokinetics. Due to this importance, engineers have sought create physiologically relevant reproducible systems tissue, considering cell-cell extracellular matrix interaction with structural physical conditions microenvironment. Extracellular vesicles (EVs) recently emerged important carriers transferring proteins genetic material between cells organs, delivery. Vascularized platforms can be ideal system studying interactions blood vessels EVs, which are crucial understanding EV-mediated substance situations. This review summarizes recent advances vascularized platforms, standard microfluidic-based techniques EV isolation characterization, studies EVs platforms. provides insights into EV-related (patho)physiological regulations facilitates development EV-based therapeutics.

Language: Английский

Regulation of the Nrf2/HO-1 axis by mesenchymal stem cells-derived extracellular vesicles: implications for disease treatment DOI Creative Commons

Hua Su,

Zhongan Wang,

Lidan Zhou

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: June 10, 2024

This comprehensive review inspects the therapeutic potential of mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) across multiple organ systems. Examining their impact on integumentary, respiratory, cardiovascular, urinary, and skeletal systems, study highlights versatility MSC-EVs in addressing diverse medical conditions. Key pathways, such as Nrf2/HO-1, consistently emerge central mediators antioxidative anti-inflammatory effects. From expediting diabetic wound healing to mitigating oxidative stress-induced skin injuries, alleviating acute lung even offering solutions for conditions like myocardial infarction renal ischemia-reperfusion injury, demonstrate promising efficacy. Their adaptability different administration routes identifying specific factors opens avenues innovative regenerative strategies. positions candidates future clinical applications, providing a overview medicine.

Language: Английский

Citations

8
Revolutionizing cancer treatment via bioengineered extracellular vesicles: Exploring nanovesicles to fully synthetic solutions DOI Creative Commons

Minmin Shao,

Juliana Rodrigues, Inês Sousa‐Oliveira

et al.

Applied Materials Today, Journal Year: 2024, Volume and Issue: 40, P. 102395 - 102395

Published: Aug. 28, 2024

The prevalence of cancer on a global scale has necessitated the development various therapeutic approaches. However, effectiveness and safety existing methods often face some limitations. Nanotechnology emerged as solution, enabling design nanosystems that can effectively deliver drugs. These address challenges such low drug stability solubility, well lack tumor targetability. While nanomaterials offer advantages, certain nanoparticles have their own limitations, which prompted researchers to explore innovative delivery systems. One promising avenue is use extracellular vesicles (EVs), are natural secreted by cells. EVs possess biocompatibility, stability, targeting capabilities, making them ideal candidates for carriers. still remain in this field. include limited production yield, complexity, inefficient cargo loading, controlled release To overcome these challenges, been employing engineering techniques modify structures EVs, enhancing intrinsic properties. Surface modification hybrid systems combine with other shown potential addressing This review focuses landscape crucial role therapeutics diagnosis. It begins exploring biological functions properties EVs. Additionally, introduces strategies isolating shedding light methodologies used harvest minute entities. Finally, highlights biomedical applications bioengineering treatment.

Language: Английский

Citations

4
Unlocking Exosome Therapeutics: The Critical Role of Pharmacokinetics in Clinical Applications DOI
Krishna Yadav, Kantrol Kumar Sahu,

Sucheta

et al.

Tissue and Cell, Journal Year: 2025, Volume and Issue: 93, P. 102749 - 102749

Published: Jan. 23, 2025

Language: Английский

Citations

0
Targeted isolation of extracellular vesicles from cell culture supernatant using immuno-affinity chromatography DOI Creative Commons
Rita P. Fernandes, Ana Ruiz,

Sandra Bezemer

et al.

Separation and Purification Technology, Journal Year: 2024, Volume and Issue: unknown, P. 130312 - 130312

Published: Oct. 1, 2024

Language: Английский

Citations

1
Approaches and Challenges in Characterizing the Molecular Content of Extracellular Vesicles for Biomarker Discovery DOI Creative Commons
Suman Kumari, Christopher Lausted, Kelsey Scherler

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(12), P. 1599 - 1599

Published: Dec. 14, 2024

Extracellular vesicles (EVs) are lipid bilayer nanoparticles released from all known cells and involved in cell-to-cell communication via their molecular content. EVs have been found tissues body fluids, carrying a variety of biomolecules, including DNA, RNA, proteins, metabolites, lipids, offering insights into cellular pathophysiological conditions. Despite the emergence contents as important biological indicators, it remains difficult to explore EV-mediated processes due small size heterogeneity technical challenges characterizing EV-associated RNAs, especially microRNAs, extensively studied. However, other less characterized protein-coding mRNAs, long noncoding circular tRNAs, also EVs. Furthermore, proteins can be used distinguish different types The spectrum well may associated with Therefore, ability comprehensively characterize EVs’ content is critical for understanding function potential applications disease diagnosis. Here, we set out provide an overview RNAs approaches currently being them.

Language: Английский

Citations

1
Vascularized platforms for investigating cell communication via extracellular vesicles DOI
Junyoung Kim, Jooyoung Ro, Yoon‐Kyoung Cho

et al.

Biomicrofluidics, Journal Year: 2024, Volume and Issue: 18(5)

Published: Sept. 1, 2024

The vascular network plays an essential role in the maintenance of all organs body via regulated delivery oxygen and nutrients, as well tissue communication transfer various biological signaling molecules. It also serves a route for drug administration affects pharmacokinetics. Due to this importance, engineers have sought create physiologically relevant reproducible systems tissue, considering cell-cell extracellular matrix interaction with structural physical conditions microenvironment. Extracellular vesicles (EVs) recently emerged important carriers transferring proteins genetic material between cells organs, delivery. Vascularized platforms can be ideal system studying interactions blood vessels EVs, which are crucial understanding EV-mediated substance situations. This review summarizes recent advances vascularized platforms, standard microfluidic-based techniques EV isolation characterization, studies EVs platforms. provides insights into EV-related (patho)physiological regulations facilitates development EV-based therapeutics.

Language: Английский

Citations

0