Multiple roles of mitochondrial autophagy receptor FUNDC1 in mitochondrial events and kidney disease DOI Creative Commons
Kaiqing Li, Xue Xia, Ying Tong

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Oct. 9, 2024

This article reviews the latest research progress on role of mitochondrial autophagy receptor FUN14 domain containing 1 (FUNDC1) in events and kidney disease. FUNDC1 is a protein located outer membrane mitochondria, which maintains function quality mitochondria by regulating autophagy, that is, selective degradation process mitochondria. The structural characteristics enable it to respond intracellular signal changes regulate activity through phosphorylation dephosphorylation. During phosphorylation, unc-51-like kinase (ULK1) promotes activation mitophagy phosphorylating Ser17 FUNDC1. In contrast, Src CK2 kinases inhibit interaction between LC3 Tyr18 Ser13, thereby inhibiting mitophagy. dephosphorylation, PGAM5 phosphatase enhances dephosphorylating activating BCL2L1 inhibits interacting with PGAM5, preventing dephosphorylation plays an important events, participating fission, maintaining homeostasis iron proteins matrix, mediating crosstalk endoplasmic reticulum lysosomes, have effects cell energy metabolism programmed death. aspect disease, abnormal closely related occurrence development many diseases. acute injury (AKI), cardiorenal syndrome (CRS), diabetic nephropathy (DN), chronic disease (CKD) ,renal fibrosis (RF) renal anemia, FUNDC1-mediated imbalance may be one key factors progression. Therefore, in-depth study regulatory mechanism great significance for understanding pathogenesis developing new treatment strategies.

Language: Английский

Targeting the NF-κB p65-MMP28 axis: Wogonoside as a novel therapeutic agent for attenuating podocyte injury in diabetic nephropathy DOI Creative Commons
Xiandeng Li, Shuyan Zhao, Jing Xie

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: 138, P. 156406 - 156406

Published: Jan. 24, 2025

Although recent progress provides mechanistic insights into diabetic nephropathy (DN), effective treatments remain scarce. DN, characterized by proteinuria and a progressive decline in renal function, primarily arises from podocyte injury, which impairs the glomerular filtration barrier. Wogonoside, bioactive compound traditional Chinese herb Scutellaria baicalensis, has not been explored for its role DN. This study aimed to investigate therapeutic effects of wogonoside on injury DN molecular mechanisms. The were examined using HFD/STZ-induced mouse models high glucose (HG)-induced MPC-5 cells. Oxidative stress inflammation markers analyzed via Western blot RT-qPCR. Wogonoside targets identified through DARTS-MS validated SPR, docking, alanine scanning, CETSA. RNA-Seq analysis was employed identify downstream targets, p65-MMP28 axis p65 knockdown overexpression studies. regulatory effect Mmp28 confirmed dual-luciferase reporter assays ChIP-qPCR. treatment significantly reduced oxidative vivo vitro. Mechanistic studies as direct target wogonoside, with SPR confirming strong binding affinity (KD = 25.05 μM). Molecular docking scanning LYS221 critical site, further supported CETSA K221A mutant. revealed effector involved HG-induced injury. Functional demonstrated that wogonoside's protective antioxidant inflammatory pathways are mediated axis. Dual-luciferase regulates transcription, ChIP-qPCR promoter binding. highlights promising candidate targeting NF-κB signaling These findings provide novel potential mechanisms, paving way development intervention.

Language: Английский

Citations

2
Rosmarinic acid-grafted gelatin nanogels for efficient diquafosol delivery in dry eye disease therapy DOI

Tinglian Zhou,

Su Li, Jiayan Zhu

et al.

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 373, P. 306 - 318

Published: July 19, 2024

Language: Английский

Citations

7
Global research hotspots and trends in oxidative stress-related diabetic nephropathy: a bibliometric study DOI Creative Commons
Xiaorong Wang,

Wu Zeng,

Tingchao He

et al.

Frontiers in Endocrinology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 10, 2025

Oxidative stress is widely acknowledged as a key pathogenic mechanism in diabetic nephropathy (DN). In recent years, the role of oxidative DN has garnered increasing attention. However, no bibliometric analysis yet been conducted on relationship between and DN. This study aims to systematically analyze relevant literature, identify trends research, assess current hotspots, predict future directions. We retrieved literature related from Web Science Core Collection database. analyzed data publication volume, countries/regions, institutions, journals, keywords, other metrics using VOSviewer, Bibliometrix R package, CiteSpace. From 2014 2024, total 4076 publications were published across 755 showing consistent upward trend each year. China United States are leading contributors this field demonstrate close collaborative efforts. The top by country, institution, journal, author include: (1919 publications), Jilin University Central South (69 each), BIOMEDICINE & PHARMACOTHERAPY (117 Prof. Sun Lin (33 publications). most frequent keyword "oxidative stress" (3683 occurrences). co-citation analysis, Alicic RZ's 2017 was cited (144 citations). These findings highlight critical importance investigating pathogenesis perspective. demonstrates steady increase research since 2014, highlighting its central Future should focus molecular mechanisms explore therapeutic potential, provide new strategies for prevention treatment

Language: Английский

Citations

0
High Rosmarinic Acid Content Melissa officinalis L. Phytocomplex Modulates Microglia Neuroinflammation Induced by High Glucose DOI Creative Commons

Giacomina Videtta,

Chiara Sasia, Nicoletta Galeotti

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(2), P. 161 - 161

Published: Jan. 29, 2025

Diabetic patients experience hyperglycemia, which can affect multiple organs, including brain function, leading to disabling neurological complications. Hyperglycemia plays a key role in promoting neuroinflammation, the most common complication diabetic individuals, through activation of microglia. Attenuating hyperglycemia-related neuroinflammation microglia may reduce diabetes-associated comorbidities. Natural remedies containing phenolic compounds have shown efficacy mitigating microglia-mediated neuroinflammation. The aim this study was investigate potential Melissa officinalis L. (MO) phytocomplex, obtained from plant cell cultures and enriched its main polyphenolic constituent, rosmarinic acid (RA), attenuating hyperglycemia-induced A time-course morphological analysis BV2 microglial cells exposed high glucose (HG) levels showed shift towards proinflammatory phenotype, peaking after 48 h, reversed by pretreatment with MO. Biochemical assays revealed increased expression marker CD11b (187%), NF-κB pathway (179%), iNOS (225%), enhanced phosphorylation ERK1/2 (180%), cytokine IL-6 (173%). Pretreatment MO prevented aberrant these mediators restored SIRT1 levels. Exposure neuronal SH-SY5Y conditioned medium HG-exposed significantly reduced viability. counteracted effect, exhibiting neuroprotective activity. RA comparable that In conclusion, attenuated oxidative imbalance under HG exposure inhibiting toward phenotype induced abrogating subsequent downstream ERK1/2–NF-κB–iNOS pathway.

Language: Английский

Citations

0
Bi2O2Se nanosheets coated with rosmarinic acid for colon-targeted treatment of inflammatory bowel disease DOI

Jiahu Yao,

Yuan‐Han Yang, Zhao Hu

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 160230 - 160230

Published: Feb. 1, 2025

Language: Английский

Citations

0
The chromosome‐scale assembly of the Salvia plebeia genome provides insight into the biosynthesis and regulation of rosmarinic acid DOI Creative Commons
Yiqun Dai, Mengqian He, Hui Liu

et al.

Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

Summary Salvia plebeia is an important traditional Chinese medicinal herb, with flavonoids and phenolic acids as its primary bioactive components. However, the absence of a reference genome hinders our understanding genetic basis underlying synthesis these Here, we present high‐quality, chromosome‐scale assembly S. , spanning 1.22 Gb, contig N50 91.72 Mb 36 861 annotated protein‐coding genes. Leveraging data, identified four catalytic enzymes—one rosmarinic acid synthase (RAS) three cytochrome P450 monooxygenases (CYP450s) —in which are involved in biosynthesis. We demonstrate that SpRAS catalyses conjugation various acyl donors acceptors, resulting formation precursor compounds. SpCYP98A75, SpCYP98A77 SpCYP98A78 catalyse from precursors at either C‐3 or C‐3′ position. Notably, SpCYP98A75 exhibited stronger hydroxylation capacity position, whereas greater efficiency Furthermore, hydroxylated both positions simultaneously, promoting conversion 4‐coumaroyl‐4′‐hydroxyphenyllactic to acid. Next, using hairy root transformation system for basic helix–loop–helix protein type transcription factor, SpbHLH54 positively regulates biosynthesis homoplantaginin . These findings provide valuable genomic resource elucidating mechanisms regulation improve evolutionary patterns within Lamiaceae family.

Language: Английский

Citations

0
Integrative analyzing the molecular basis of Clerodendranthus Spicatus aqueous extract in hyperuricemia nephropathy via network pharmacology and experimental validation DOI
Chuang Li,

Danyao Lin,

Jianting Ouyang

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106152 - 106152

Published: Feb. 1, 2025

Language: Английский

Citations

0
Application and Progression of Single‐Cell RNA Sequencing in Diabetes Mellitus and Diabetes Complications DOI Creative Commons

Jiajing Hong,

Shiqi Lu,

Guohui Shan

et al.

Journal of Diabetes Research, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Diabetes is a systemic metabolic disorder primarily caused by insulin deficiency and resistance, leading to chronic hyperglycemia. Prolonged diabetes can result in damage multiple organs, including the heart, brain, liver, muscles, adipose tissue, thereby causing various fatal complications such as diabetic retinopathy, cardiomyopathy, nephropathy. Single‐cell RNA sequencing (scRNA‐seq) has emerged valuable tool for investigating cell diversity pathogenesis of identifying potential therapeutic targets or complications. This review provides comprehensive overview recent applications scRNA‐seq diabetes‐related researches highlights novel biomarkers immunotherapy with cell‐type information its associated

Language: Английский

Citations

0
Discovery of Novel Rigid STING PROTAC Degraders as Potential Therapeutics for Acute Kidney Injury DOI
Rui Ma,

Renquan Fu,

Yifan Wang

et al.

European Journal of Medicinal Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 117539 - 117539

Published: March 1, 2025

Language: Английский

Citations

0
Gut Microbiota and Their Metabolites: The Hidden Driver of Diabetic Nephropathy? Unveiling Gut Microbe's Role in DN DOI Creative Commons

J. Liu,

Min Guo, Xiaobin Yuan

et al.

Journal of Diabetes, Journal Year: 2025, Volume and Issue: 17(4)

Published: April 1, 2025

ABSTRACT Background Diabetic nephropathy (DN) is a severe microvascular complication of diabetes with complex pathogenesis. Methods Recent studies were reviewed to explore the role gut microbiota and its metabolites in DN development. Results Dysbiosis bacteria contributes pathological changes such as glomerular sclerosis renal tubule injury. Microbial are involved through immune inflammatory pathways. Conclusions Understanding relationship between microbiota, metabolites, may offer potential implications for diagnosis, prevention, treatment. Translating this knowledge into clinical practice presents challenges opportunities.

Language: Английский

Citations

0