Gut Microbes,
Journal Year:
2025,
Volume and Issue:
17(1)
Published: April 7, 2025
Ulcerative
colitis
(UC)
poses
significant
threats
to
human
health
and
quality
of
life
worldwide,
as
it
is
a
chronic
inflammatory
bowel
disease.
3'-sialyllactose
(3'-SL)
key
functional
component
milk
oligosaccharides.
This
study
systematically
evaluates
the
prebiotic
effects
3'-SL
its
therapeutic
potential
in
combination
with
Bifidobacterium
infantis
(B.
infantis)
for
UC.
The
findings
reveal
that
B.
synergistically
mitigate
intestinal
inflammation
barrier
dysfunction
by
promoting
production
short-chain
fatty
acids
(SCFAs)
through
cross-feeding
mechanisms
among
gut
microbiota.
Individually,
3'-SL,
infantis,
synbiotic
treatment
all
effectively
alleviated
UC
symptoms,
including
reduced
weight
loss,
improved
disease
activity
scores,
prevention
colon
shortening.
Histopathological
immunofluorescence
analyses
further
demonstrated
significantly
ameliorated
colonic
injury,
enhanced
function,
restored
goblet
cell
counts,
increased
glycoprotein
content
crypt
cells,
upregulated
expression
tight
junction
proteins
(ZO-1,
occludin,
claudin-1).
Notably,
outperformed
individual
components
better
restoring
microbiota
balance,
elevating
SCFA
levels,
modulating
serum
cytokine
profiles,
thereby
reducing
inflammation.
These
provide
mechanistic
insights
into
protective
underscore
other
disorders.
Frontiers in Pharmacology,
Journal Year:
2024,
Volume and Issue:
15
Published: Oct. 28, 2024
Background
Clinical
observations
have
recently
shown
that
Abelmoschus
manihot
(L.)
in
the
form
of
Huangkui
capsule
(HKC)
and
combination
with
irbesartan
(EB)
is
an
effective
therapy
for
diabetic
nephropathy
(DN)
patients
type
2
diabetes
(T2D).
The
present
study
aims
to
explore
mechanisms
underlying
therapeutic
efficacies
HKC
its
EB
DN
via
gut-kidney
axis.
Methods
HKC,
EB,
their
or
vehicle
were
administered
db/db
mice,
which
animal
model
T2D
DN.
Comparative
analyses
gut
microbiota,
serum
metabolites,
kidney
transcriptomics
before
after
drug
administration
performed.
Results
After
treatment
4
weeks,
urinary
albumin-to-creatinine
ratios
decreased
significantly
mice
In
terms
abundances
Faecalitalea,
Blautia,
Streptococcus
increased
but
those
Bacteroidetes,
Firmicutes,
Enterobacteriaceae,
Desulfovibrio
decreased.
Parallelly,
mainly
including
quercetin
3′-glucuronide
L-dopa,
elevated
while
cortisol
cytochalasin
B
reduced.
Furthermore,
S100a8
,
S100a9
Trem1
Mmp7
genes
kidneys
downregulated.
These
altered
elements
associated
proteinuria/albuminuria
reduction.
However,
had
no
effects
on
changes
blood
pressure
specific
differentially
expressed
kidneys.
Conclusion
provides
experimental
evidence
regulates
circulating
renal
gene
activities,
are
useful
better
understanding
action
A.
through
