Glycoside-Mediated Enhancement of Stability in Aluminum Oxyhydroxide Nanoadjuvants during Freeze-Drying DOI

Mudasira Bhurt,

Xin Li, Nan Zhang

et al.

Langmuir, Journal Year: 2024, Volume and Issue: 40(46), P. 24613 - 24621

Published: Nov. 6, 2024

Aluminum-based adjuvants have been indispensable to vaccine potency. However, their effectiveness is difficult maintain after freeze-drying, which limits the storage and application of aluminum-adjuvanted vaccines. In this study, impact freeze-drying on aluminum oxyhydroxide nanorods (AlOOH NRs) was investigated. Freeze-drying led aggregation resulted in loss surface hydroxyl content adjuvants. To alleviate freeze-drying-induced damage, potency different alkyl glycosides as protectants further evaluated. It demonstrated that structural balance head tail a glycoside more conducive protecting AlOOH NRs from groups. These results underline proper selection protect against functional defects caused by important for stability efficacy vaccines biopharmaceutical products.

Language: Английский

Artificially Tagging Tumors with Nano-Aluminum Adjuvant-Tethered Antigen mRNA Recruits and Activates Antigen-Specific Cytotoxic T Cells for Enhanced Cancer Immunotherapy DOI Creative Commons
Lingxiao Zhang, Jie Bai,

Aining Shen

et al.

Biomaterials, Journal Year: 2025, Volume and Issue: 317, P. 123085 - 123085

Published: Jan. 5, 2025

T cell therapy for solid tumors faces significant challenges due to the immune off-target attack caused by loss of tumor surface antigens and inactivation in acidic microenvironment (TME). Herein, we developed a bifunctional immunomodulator (MO@NAL) loading ovalbumin (OVA; model antigen) mRNA (mOVA) onto lysozyme-coated layered double hydroxide nano-aluminum adjuvant (NA). The NA's inherent alkalinity effectively neutralizes excess acid within TME suppresses regulatory cells, creating favorable enhance cytotoxic infiltration activation tumors. Particularly, once internalization MO@NAL efficiently tags with OVA through carried mOVA, providing targets recruiting directing antigen-specific cells destroy cells. In mice pre-vaccinated vaccine, intratumoral administration rapidly awakens OVA-specific memory, inhibiting progression colon melanoma at both early advanced stages. non-pre-vaccinated mice, combining therapeutic vaccine or adoptive transfusion similarly achieves robust suppression. These findings thus underscore potential as an effective safe enhancing responses timely intervention progression.

Language: Английский

Citations

0
Bioengineered ClearColi™-derived outer membrane vesicles displaying CT26 neoepitopes as potent vaccine adjuvants against colon carcinoma in a preventive mouse model DOI
Elham Sharif, Taranom Mobasheri, Elham Mohit

et al.

Vaccine, Journal Year: 2025, Volume and Issue: 53, P. 127088 - 127088

Published: April 1, 2025

Language: Английский

Citations

0
Chiral Aluminum Oxyhydroxide Supraparticles as Adjuvants DOI
Zongda Li,

Aihua Qu,

Chuanlai Xu

et al.

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Abstract Aluminum‐based adjuvants dominate global vaccine formulations owing to their proven efficacy in humoral immunity induction. However, inherent limitations activating cellular pose critical challenges for development. In this study, chiral flower‐like aluminum oxyhydroxide (AlOOH) supraparticles (SPs) are synthesized via a one‐pot hydrothermal method using cysteine (Cys) enantiomers as ligands, achieving g‐factor of 0.004. L‐AlOOH SPs (L‐SPs) demonstrate significantly greater enhancement dendritic cell (DC) maturation and antigen cross‐presentation efficiency compared D‐AlOOH (D‐SPs), indicating its potential an adjuvant. Mechanistic studies reveal that L‐SPs enter DCs Toll‐like receptor 2 (TLR2), thereby enhancing NOD‐like thermal protein domain associated 3 (NLRP3) inflammasome activation. vivo experiments show generate 21.59‐fold higher OVA‐specific antibody titers than commercial adjuvants. Further L‐SPs, after mixed with H9N2 virus proteins, enhance influenza by 15.28‐fold, sustained protection, confirming translational potential. This study demonstrates the performance AlOOH simultaneously amplify immunological responses, entering it promising next‐generation adjuvant cancer immunotherapy pandemic preparedness.

Language: Английский

Citations

0
Glycoside-Mediated Enhancement of Stability in Aluminum Oxyhydroxide Nanoadjuvants during Freeze-Drying DOI

Mudasira Bhurt,

Xin Li, Nan Zhang

et al.

Langmuir, Journal Year: 2024, Volume and Issue: 40(46), P. 24613 - 24621

Published: Nov. 6, 2024

Aluminum-based adjuvants have been indispensable to vaccine potency. However, their effectiveness is difficult maintain after freeze-drying, which limits the storage and application of aluminum-adjuvanted vaccines. In this study, impact freeze-drying on aluminum oxyhydroxide nanorods (AlOOH NRs) was investigated. Freeze-drying led aggregation resulted in loss surface hydroxyl content adjuvants. To alleviate freeze-drying-induced damage, potency different alkyl glycosides as protectants further evaluated. It demonstrated that structural balance head tail a glycoside more conducive protecting AlOOH NRs from groups. These results underline proper selection protect against functional defects caused by important for stability efficacy vaccines biopharmaceutical products.

Language: Английский

Citations

0