Analysis on Bacterial Community of Noctiluca scintillans Algal Blooms Near Pingtan Island, China

Biology, Journal Year: 2025, Volume and Issue: 14(1), P. 101 - 101

Published: Jan. 20, 2025

Noctiluca scintillans, known as a global red tide species, is common species found in Pingtan Island. To examine the bacterial community structure different environments during period of N. scintillans on Island, samples were collected from Algal Bloom Area (ABA), Transition (TA), and Non-Algal (NBA) 6 April 2022, environmental physicochemical factors determined. The outbreak significantly impacted water quality affected sea area. ABA has deteriorated markedly, with contents COD, NH4+-N, PO43− being higher than those TA NBA, while pH lower that NBA. richness, diversity, evenness are all For instance, Shannon index values three sampling points 4.41, 5.41, 6.37, respectively. At phylum level, dominant phyla Proteobacteria, Firmicutes, Cyanobacteria; TA, they Bacteroidetes, Firmicutes; Cyanobacteria. genus genera Vibrio, Carnobacterium, Candidatus_Megaira, Planktomarina, Pseudoalteromonas; Lentibacter, Glaciecola, Jannaschia; Amylibacter, NS5_marine_group, Aurantivirga, marine_metagenome. In ABA, combined proportion Vibrio Carnobacterium exceeds 50%, Vibrio_splendidus accounting for 93% total population. These research results can provide scientific basis clarifying characteristics composition large-scale

Language: Английский

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Highly Pleomorphic Strains of the Vibrio Predator Pseudoalteromonas piscicida and Their Outer Membrane Vesicles: A Scanning Electron Micrographic Study

Microorganisms, Journal Year: 2025, Volume and Issue: 13(2), P. 365 - 365

Published: Feb. 7, 2025

Pseudoalteromonas species are recognized for their probiotic roles in reducing pathogens aquaculture products by secreting a broad range of antimicrobial compounds. Some species, like P. piscicida, also predators that attack susceptible prey bacteria, including V. parahaemolyticus, transferring outer membrane vesicles (OMVs) containing digestive compounds to the surface prey. These digest holes prey's cell wall releasing nutrients upon which feed. In present study, scanning electron microscopy was performed on two piscicida strains grown sterile seawater and nutrient-enriched seawater, without presence bacterial prey, determine if or low-nutrient media required induce vesicle formation. Micrographs revealed OMV formation high pleomorphism absence cells regardless nutrient levels seawater. Phenotypic characteristics included (i) vesiculated non-vesiculated (ii) large bulbous versus small OMV, (iii) pilus-like connectors widely varying lengths were attached, (iv) highly elongated (10 µm long) cells, (v) appeared extend 50 long be septating dividing into short chains individual cells. The possible contribution these novel phenotypes predation is discussed.

Language: Английский

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Vibrio harveyiplasmids as drivers of virulence in barramundi (Lates calcarifer)?

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 7, 2025

Abstract Vibrio species are an emerging public and animal health risk in marine environments the opportunistic bacterial pathogen harveyi is a major disease for tropical aquaculture. Current understanding of virulence V. limited by strain-specific variability complex host-pathogen dynamics. This study sought to integrate genomic investigation, phenotypic characterisation vivo challenge trials barramundi ( Lates calcarifer) increase our virulence. We identified two hypervirulent isolates, Vh-14 Vh-15 that caused 100% mortality fish within 48 hours, were phenotypically genotypically distinct from other isolates. Virulent isolates contained multiple plasmids, including 105,412 bp conjugative plasmid with type III secretion system genes originally Yersinia pestis . The emergence this plasmid-mediated patho-variant poses potential threat sustainable production finfish Southeast Asia, Mediterranean Australia. In addition, we observed effect temperature on indicators activity at 28°C 34°C compared 22°C. suggests fluctuations associated climate change may act as stressor bacteria, increasing gene host adaptation. Our results utilising myriad technologies tools, highlights importance holistic view characterisation.

Language: Английский

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Isolation, characterization and genomic analysis of bacteriophages for biocontrol of vibriosis caused by Vibrio alginolyticus

Virus Research, Journal Year: 2025, Volume and Issue: 353, P. 199529 - 199529

Published: Feb. 11, 2025

Language: Английский

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Evaluation of protective immune response of live-attenuated candidate vaccines ΔcpxA and ΔcpxR against Vibrio alginolyticus in pearl gentian grouper

Fish & Shellfish Immunology, Journal Year: 2025, Volume and Issue: unknown, P. 110183 - 110183

Published: Feb. 1, 2025

Language: Английский

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Proteomics analysis of extracellular vesicles during Vibrio anguillarum infection in lumpfish (Cyclopterus lumpus)

Fish & Shellfish Immunology, Journal Year: 2025, Volume and Issue: unknown, P. 110205 - 110205

Published: Feb. 1, 2025

Language: Английский

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Exploration of shrimp and their environments for the detection of antibiotic resistance genes of Vibrio parahaemolyticus and spectrophotometry of shrimp muscles for heavy metals and their human health risk assessment in Bangladesh

Journal of Food Protection, Journal Year: 2025, Volume and Issue: unknown, P. 100475 - 100475

Published: Feb. 1, 2025

Language: Английский

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Pathology of an unusual outbreak of fatal disease associated with mycobacterial and Pseudomonas aeruginosa infections and intracytoplasmic inclusion bodies in juvenile spectacled caimans (Caiman crocodilus)

Journal of Comparative Pathology, Journal Year: 2025, Volume and Issue: 218, P. 19 - 25

Published: March 12, 2025

Language: Английский

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Homologous relationship between FabG involved in fatty acid biosynthesis and SDR on chromosome II in the multi-chromosome pathogen Vibrio anguillarum

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 13, 2025

Vibrio anguillarum threatens fish and larval farming industries human health worldwide. The identification of bacterial adaptation responses to stress due environmental changes is vital for establishing a response strategy pathogenic Vibrio. Previously, short-chain dehydrogenase/reductase (SDR) was identified on chromosome II the multichromosomal V. anguillarum. In this study, comparison SDR enzyme FabG-1b (encoded I responsible β-ketoacyl acyl carrier protein (ACP) reductase in fatty acid biosynthesis (FAS II)) showed that amino sequence homology only 33.2%; however, core functionality, which includes NAD(P)-binding domain conserved region active site, topologies predicted using sequence-based modeling, quaternary homotetramer-type structures significant similarity. specific substrates fluorinated halogenated aliphatic ketones, aromatic β-ketoesters toward non-fluorinated non-halogenated non-aromatic β-ketoesters. This complementary catalytic efficiencies two enzymes various conclusively supports hypothesis are likely homologs. first study report potential paralogous information improves our understanding FAS strategies overcome infectious diseases caused by strains identify targets developing new antibacterial agents.

Language: Английский

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A suite of ddPCR assays targeting microbial pathogens for improved management of shellfish aquaculture

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

The shellfish aquaculture industry is one of the fastest-growing sectors global food production, but it currently facing major challenges stemming from microbial pathogens. This study presents an optimized and validated suite droplet digital PCR (ddPCR) assays using water samples proximal to oyster farms in North Carolina quantify pathogens relevant industry. Two molecular enable quantification pathogens, Vibrio parahaemolyticus Perkinsus marinus, that threaten human health performance, respectively. work also introduces two ddPCR simultaneous at least nine ecologically spp. only sets primers probes targeting glycosyl hydrolase family 18 (GH18) domain chiA gene bacteria. entire was applied single assessments 12 sites, revealing heterogeneity pathogen concentrations across coastal landscape variability abundances within individual estuarine river systems. Additionally, a longitudinal conducted demonstration lease elucidated unique temporal trends for all targets. Notably, when quantified reached their maximum, daily probability mortality increased, suggesting role other pertinent progression would otherwise be missed. highlights utility advancement management by offering insights into spaciotemporal dynamics Climate change drastically altering environment changing abundance geographical distribution marine These species put additional pressure on acting as sources disease animals important well humans upon consumption contaminated food. To address growing concerns, high-resolution monitoring can offer effective critical Validated field, presented here improves current methods, enabling several technology makes possible track they move through reveals changes may inform adjustments farming practices aimed mitigating negative outcomes. this approach assay design unveils potential drivers ecological shifts emerging etiologies more efficiently.

Language: Английский

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