Cyanobacteria Desertifilum tharense NIOF17/006 as a novel aquafeed additive: effect on the growth, immunity, digestive function, and gene expression of whiteleg shrimp postlarvae

Frontiers in Marine Science, Journal Year: 2025, Volume and Issue: 12

Published: March 12, 2025

This work investigated the molecular identification and potential application of cyanobacterial strain Desertifilum tharense NIOF17/006 as a novel aquafeed additive for whiteleg shrimp ( Litopenaeus vannamei ) postlarvae (PLs). Morphological characterization confirmed isolate D. , with 16S rRNA sequence analysis showing high similarity (98.01%–98.53%) to known strains . Biochemical revealed that contains 37.74% protein, 5.52% lipid, 21.25% carbohydrate, on dry weight basis. An 8-week feeding trial L. PLs evaluated effects dietary supplementation at doses 0, 1, 2.5, 5 g/kg diet. Compared in control group, fed tharens e-supplemented diets had significantly higher feed utilization, growth performance, survival rate, whole body composition. The nonspecific immunity parameters (i.e., lysozyme, superoxide dismutase, catalase), well digestive enzyme activity amylase lipase, were enhanced supplemented cyanobacteria, while malondialdehyde (MDA) levels decreased. gene expression upregulation growth-related genes hormone insulin-like factor I II immune-related prophenoloxidase proPO ), dismutase SOD lysozyme Lys muscles increasing cyanobacteria supplementation, particularly 2.5–5 Moreover, polynomial regression machine learning model predicts ideal level probiotic ranges from 3.4 4.2 study demonstrates promising improvement growth, immunity, overall health PLs, opening new avenue sustainable aquaculture practices.

Language: Английский

A study on the functional role of the DHCR24 gene in gonadal differentiation and development of Macrobrachium nipponense

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 27, 2024

Sex differentiation in crustaceans is a complex process influenced by various factors, including the androgenic gland and sex-related genes. This study characterized role of Mn-DHCR24 gene oriental river prawn (Macrobrachium nipponense). We used bioinformatics to analyze sequence features phylogenetic relationships single gene. The expression patterns across different tissues developmental stages were determined real-time PCR, its localization testis was situ hybridization. RNA interference knock down expression, followed examining changes sex ratio gonadal development at PL10 stage. Additionally, an enzyme-linked immunosorbent assay measured 17α-methyltestosterone levels, tissue sections characterize development. results indicated that high testis, which critical for sperm maturation differentiation. RNAi experiments showed during reproductive regulation rather than as master further regulated hormone-related genes, influencing steroid biosynthesis pathways. Together, these findings provided valuable insights into genetic hormonal mechanisms M. nipponense, supported monosex culture technology.

Language: Английский